湿度调控设施黄瓜棒孢叶斑病菌产孢和释放规律及防治技术

doi:10.3864/j.issn.0578-1752.2023.15.006

Abstract

Abstract:

【Objective】 Cucumber target leaf spot, caused by Corynespora cassiicola, has brought great economic losses to the cucumber industry. Sporulation and release of C. cassiicola spores play a significant role in the epidemiology of the disease. In this study, the regularity and the effect of humidity on sporulation and release of C. cassiicola, and the optimal application method and time for control of cucumber target leaf spot were evaluated.【Method】 The sporulation regularity of C. cassiicola was evaluated by quantifying the spore concentration on lesions of diseased cucumber leaves at 0: 00, 3: 00, 6: 00, 9: 00, 12: 00, 15: 00, 18: 00, and 21: 00, respectively. The release regularity of C. cassiicola was evaluated in different seasons of spring, summer, autumn and winter, air samples were collected from naturally infested cucumber greenhouse at 0: 00, 3: 00, 6: 00, 9: 00, 12: 00, 15: 00, 18: 00, and 21: 00, respectively, and the concentrations of C. cassiicola in the air were evaluated. The effect of relative humidity on sporulation and release of C. cassiicola was also assessed in artificial climate exposure chambers and plastic greenhouses at four different humidity conditions of continuous high humidity (RH>90%, 24 h), continuous low humidity (RH<60%, 24 h), high humidity for 12 h followed by low humidity for 12 h, and low humidity for 12 h followed by high humidity for 12 h. The control efficiency of 60% carbendazim·diethofencarb wettable powder (WP) and 500 million spores/g Pseudomonas fluorescens WP on cucumber target leaf spot disease was compared, by using powder spraying and water spraying at different application times in the field.【Result】 The study on the daily variation regularity of sporulation and release of C. cassiicola showed significant differences in the quantity of spores on diseased leaves and in the greenhouse air at different times of the day. There was a complementary relationship between the number of spores on diseased leaves and in the greenhouse air at the same time. After 18: 00, as the duration of high humidity (RH>90%) prolonged, the number of spores on diseased leaves increased, reaching a peak of 1 344 spores/cm² at 6: 00 the next day. Then, the humidity decreased gradually to RH<60% after opening the air vent of the greenhouse, and spores were released into the greenhouse space. At 12: 00, the spore concentrations in the greenhouse air reached a peak of 12 445-110 697 spores/m³. In different seasons of spring, summer, autumn, and winter, the daily variation regularity of sporulation and release is consistent, showing that C. cassiicola produced a large amount of spores under high humidity (RH>90%) at night, and released to greenhouses under low humidity (RH<60%) during the day. In artificial climate exposure chambers and plastic greenhouses, the highest quantity of C. cassiicola spores was detected under alternating wet and dry conditions, which was significantly higher than that under continuous high humidity or continuous low humidity condition. By powder spraying at 19: 00, 60% carbendazim·diethofencarb WP and 500 million spores/g P. fluorescens WP gave the best control efficiency of 80.60% and 75.08%, respectively, and the spore inhibition efficiency was higher than 84%.【Conclusion】 Humidity is a key environmental factor affecting the spore reproduction and release of C. cassiicola. The alternating day-dry and night-wet environment in the greenhouse promotes the reproduction and diffusion of C. cassiicola, and accelerates the spread of cucumber target leaf spot. Powder spraying method is better than water spraying method for disease control, and the best application time is the evening before spore reproduction. The results of this study will contribute to the development of new strategies for the effective alleviation and control of cucumber target leaf spot.

Key words: cucumber target leaf spot, Corynespora cassiicola, humidity, sporulation, release, powder spraying, application time

CHAI ALi, YANG HongMin, WANG ShaoHua, ZHAO Kun, GAO Wei, SHI YanXia, XIE XueWen, LI Lei, FAN TengFei, LI BaoJu. Effect of Humidity on Sporulation and Release of Corynespora cassiicola and Control Technology[J].Scientia Agricultura Sinica, 2023, 56(15): 2907-2918.

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References 38

[1]	王泉城, 武军, 李磊, 石延霞, 谢学文, 李宝聚, 柴阿丽. 多主棒孢菌CcTLS1对黄瓜的致病机理分析. 园艺学报, 2023, 50(3): 569-582. doi: 10.16420/j.issn.0513-353x.2021-1056
	WANG Q C, WU J, LI L, SHI Y X, XIE X W, LI B J, CHAI A L. Exploration on the function of pathogenicity-related gene CcTLS1 in Corynespora cassiicola from cucumber. Acta Horticulturae Sinica, 2023, 50(3): 569-582. (in Chinese)
[2]	SANCHEZ CASTRO M A. Leaf blight caused by Corynespora: A new disease on cucumber (Cucumis sativus) in the Valley of Culiacan, Sinaloa, Mexico, and its chemical control. Plant Disease Reporter, 1979, 63(7): 599-601.
[3]	DIXON L J, SCHLUB R L, PERNEZNY K, DATNOFF L E. Host specialization and phylogenetic diversity of Corynespora cassiicola. Phytopathology, 2009, 99(9): 1015-1027. doi: 10.1094/PHYTO-99-9-1015
[4]	BOOSALIS M G, HAMILTON R I. Root and stem rot of soybean caused by Corynespora cassiicola (Berk.& Curt.). Wei. Plant Disease, 1957, 41: 696-698.
[5]	BOWEN K L, HAGAN A K, PEGUES M, JONES J, MILLER H B. Epidemics and yield losses due to Corynespora cassiicola on cotton. Plant Disease, 2018, 102(12): 2494-2499. doi: 10.1094/PDIS-03-18-0382-RE
[6]	CHEE K H. Studies on sporulation, pathogenicity and epidemiology of Corynespora cassiicola on Hevea rubber. Natural Rubber Research, 1988, 3: 21-29.
[7]	CHUNG S R, LEE H S, YOU I C. Fungal keratitis caused by Corynespora cassiicola, a plant pathogen. Journal of Mycology and Infection, 2018, 23: 24-26. doi: 10.17966/jmi
[8]	MIYAMOTO T, ISHII H, SEKO T, KOBORI S, TOMITA Y. Occurrence of Corynespora cassiicola isolates resistant to boscalid on cucumber in Ibaraki Prefecture, Japan. Plant Pathology, 2009, 58(6): 1144-1151. doi: 10.1111/ppa.2009.58.issue-6
[9]	李宝聚, 高苇, 石延霞, 谢学文. 多主棒孢和棒孢叶斑病的研究进展. 植物保护学报, 2012, 39(2): 171-176.
	LI B J, GAO W, SHI Y X, XIE X W. Progress in researches on Corynespora leaf spot. Acta Phytophylacica Sinica, 2012, 39(2): 171-176. (in Chinese)
[10]	陈璐, 石延霞, 谢学文, 柴阿丽, 李宝聚. 黄瓜棒孢叶斑病菌PCR检测方法的建立. 园艺学报, 2014, 41(3): 585-592.
	CHEN L, SHI Y X, XIE X W, CHAI A L, LI B J. PCR assay for detection of Corynespora cassiicola, the causal agent of Corynespora leaf spot of cucumber. Acta Horticulturae Sinica, 2014, 41(3): 585-592. (in Chinese)
[11]	SUN B, ZHU G, XIE X, CHAI A, LI L, SHI Y, LI B. Double mutations in succinate dehydrogenase are involved in SDHI resistance in Corynespora cassiicola. Microorganisms, 2022, 10(1): 132. doi: 10.3390/microorganisms10010132
[12]	ZHAO Q, SHI Y X, WANG Y H, XIE X W, LI L, GUO L Y, CHAI A L, LI B J. Quantifying airborne dispersal route of Corynespora cassiicola in greenhouses. Frontiers in Microbiology, 2021, 12(12): 716758. doi: 10.3389/fmicb.2021.716758
[13]	李宝聚, 陈立芹, 孟伟军, 王福建. 湿度调控对番茄灰霉病菌侵染的影响. 植物病理学报, 2003, 33(2): 167-169.
	LI B J, CHEN L Q, MENG W J, WANG F J. Effects of humidity regulation on the infection of Botrytis cinerea to tomato. Acta Phytopathologica Sinica, 2003, 33(2): 167-169. (in Chinese)
[14]	石延霞, 李宝聚, 刘学敏. 黄瓜霜霉病菌侵染若干因子的研究. 应用生态学报, 2005, 16(2): 257-261.
	SHI Y X, LI B J, LIU X M. Several infection factors of Pseudoperonospora cubensis. Chinese Journal of Applied Ecology, 2005, 16(2): 257-261. (in Chinese)
[15]	SUN S L, LIAN S, FENG S L, DONG X L, WANG C X, LI B H, LIANG W X. Effects of temperature and moisture on sporulation and infection by Pseudoperonospora cubensis. Plant Disease, 2017, 101(4): 562-567. doi: 10.1094/PDIS-09-16-1232-RE
[16]	TRAIL F, XU H X, LORANGER R, GADOURY D. Physiological and environmental aspects of ascospore discharge in Gibberella zeae (anamorph Fusarium graminearum). Mycologia, 2002, 94(2): 181-189. doi: 10.1080/15572536.2003.11833223
[17]	JOHANSSON V, LONNELL N, RANNIK Ü, SUNDBERG S, HYLANDER K. Air humidity thresholds trigger active moss spore release to extend dispersal in space and time. Functional Ecology, 2016, 30(7): 1196-1204. doi: 10.1111/fec.2016.30.issue-7
[18]	XIN X F, NOMURA K, AUNG K, VELASQUEZ A C, YAO J, BOUTROT F, CHANG J H, ZIPFEL C, HE S Y. Bacteria establish an aqueous living space in plants crucial for virulence. Nature, 2016, 539(7630): 524-529. doi: 10.1038/nature20166
[19]	LIU F, LI B H, LIAN S, DONG X L, WANG C X, ZHANG Z F, LIANG W X. Effects of temperature and moisture on the infection and development of apple fruit rot caused by Phytophthora cactorum. Plant Disease, 2018, 102(9): 1811-1819. doi: 10.1094/PDIS-07-17-1028-RE
[20]	ZHAO Q, SHI Y X, WANG Y K, XIE X W, LI L, FAN T F, GUO L Y, CHAI A L, LI B J. Temperature and humidity regulate sporulation of Corynespora cassiicola that is associated with pathogenicity in cucumber (Cucumis sativus L.). Biology, 2022, 11(11): 1675. doi: 10.3390/biology11111675
[21]	CHAI A L, YUAN L F, LI L, SHI Y X, XIE X W, WANG Q, LI B J. Aerosol transmission of Pseudomonas amygdali pv. lachrymans in greenhouses. Science of the Total Environment, 2020, 748: 141433. doi: 10.1016/j.scitotenv.2020.141433
[22]	ABDEL-HAMEED A A, KHODER M I, IBRAHIM Y H, SAEED Y, OSMAN M E, GHANEM S. Study on some factors affecting survivability of airborne fungi. Science of the Total Environment, 2012, 414: 696-700. doi: 10.1016/j.scitotenv.2011.10.042
[23]	BROWN J K, HOVMOLLER M S. Aerial dispersal of pathogens on the global and continental scales and its impact on plant disease. Science, 2002, 297(5581): 537-541. doi: 10.1126/science.1072678 pmid: 12142520
[24]	MEYER M, COX J A, HITCHINGS M D T, BURGIN L, HORT M C, HODSON D P, GILLIGAN C A. Quantifying airborne dispersal routes of pathogens over continents to safeguard global wheat supply. Nature Plants, 2017, 3(10): 780-786. doi: 10.1038/s41477-017-0017-5 pmid: 28947769
[25]	NELSON R R, TUNG G. Influence of some climatic factors on sporulation by an isolate of race T of Helminthosporium maydis on a susceptible malesterile corn hybrid. Plant Disease Reporter, 1973, 57: 304-307.
[26]	ARTHURS S, THOMAS M B. Effects of temperature and relative humidity on sporulation of Metarhizium anisopliae var. acridum in Mycosed Cadavers of Schistocerca gregaria. Journal of Invertebrate Pathology, 2001, 78(2): 59-65. doi: 10.1006/jipa.2001.5050
[27]	LAWRENCE E G, ZEHR E I. Environmental effects on the development and dissemination of Cladosporium carpophilum on peach. Phytopathology, 1982, 72(7): 773-776. doi: 10.1094/Phyto-72-773
[28]	LALANCETTE N, FOSTER K A, ROBISON D M. Quantitative models for describing temperature and moisture effects on sporulation of Phomopsis amygdali on peach. Phytopathology, 2003, 93(9): 1165-1172. doi: 10.1094/PHYTO.2003.93.9.1165
[29]	LALANCETTE N, MCFARLAND K A, BURNETT A L. Modeling sporulation of Fusicladium carpophilum on nectarine twig lesions: Relative humidity and temperature effects. Phytopathology, 2012, 102(4): 421-428. doi: 10.1094/PHYTO-08-10-0222
[30]	WARD S V, MANNERS J G. Environmental effects on the quantity and viability of conidia produced by Erysiphe graminis. Transactions of the British Mycological Society, 1974, 62(1): 119-128. doi: 10.1016/S0007-1536(74)80013-6
[31]	REUVENI R, ROTEM J. Effect of humidity on epidemiological patterns of the powdery mildew (Sphaerotheca fuliginea) on squash. Phytoparasitica, 1974, 2(1): 25-33. doi: 10.1007/BF02981068
[32]	LI X, LI B H, LIAN S, DONG X L, WANG C X, LIANG W X. Effects of temperature, moisture and nutrition on conidial germination, survival, colonization and sporulation of Trichothecium roseum. European Journal of Plant Pathology, 2019, 153(2): 557-570. doi: 10.1007/s10658-018-1583-8
[33]	JAT M K, AHIR R R. Effect of temperature, relative humidity and pH on mycelial growth and sporulation of Fusarium solani causing root rot of Indian Aloe (Aloe barbadensis Mill.). Journal of Pineal Research, 2013, 29: 181-183.
[34]	INGOLD C T. A gas phase in viable fungal spores. Nature, 1956, 177(4522): 1242-1243. doi: 10.1038/1771242a0
[35]	揣红运, 石延霞, 柴阿丽, 杨杰, 谢学文, 李宝聚. 10%乙霉威·腐霉利微粉剂的研制及其对黄瓜棒孢叶斑病的防治效果. 中国农业科学, 2019, 52(6): 1009-1020. doi: 10.3864/j.issn.0578-1752.2019.06.005. doi: 10.3864/j.issn.0578-1752.2019.06.005
	CHUAI H Y, SHI Y X, CHAI A L, YANG J, XIE X W, LI B J. Development of 10% diethofencarb·procymidone micropowder and its control efficacy to cucumber Corynespora leaf spot. Scientia Agricultura Sinica, 2019, 52(6): 1009-1020. doi: 10.3864/j.issn.0578-1752.2019.06.005. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.06.005
[36]	揣红运, 谢学文, 石延霞, 柴阿丽, 李宝聚. 枯草芽胞杆菌微粉剂的研制及其对黄瓜白粉病的防治效果. 植物病理学报, 2019, 49(5): 660-669.
	CHUAI H Y, XIE X W, SHI Y X, CHAI A L, LI B J. Preparation of micropowder of Bacillus subtilis and its control effect on cucumber powdery mildew. Acta Phytopathologica Sinica, 2019, 49(5): 660-669. (in Chinese)
[37]	谢学文, 揣红运, 李磊, 柴阿丽, 石延霞, 李宝聚. 10%乙霉威·腐霉利微粉剂在设施黄瓜上的沉积分布及残留消解动态. 农药学学报, 2020, 22(2): 378-387.
	XIE X W, CHUAI H Y, LI L, CHAI A L, SHI Y X, LI B J. Deposition distribution and dissipation dynamics of 10% diethofencarb + procymidone micropowder in facility cucumber. Chinese Journal of Pesticide Science, 2020, 22(2): 378-387. (in Chinese)
[38]	苑宝洁, 李磊, 李新宇, 杨文, 石延霞, 柴阿丽, 张红杰, 谢学文, 李宝聚. 解淀粉芽胞杆菌ZF57微粉剂的研制及对黄瓜棒孢叶斑病的防治效果. 植物病理学报, 2022, 52(2): 235-246.
	YUAN B J, LI L, LI X Y, YANG W, SHI Y X, CHAI A L, ZHANG H J, XIE X W, LI B J. Preparation of Bacillus amyloliquefaciens ZF57 micropowder and its control effect on cucumber coryneform leaf spot. Acta Phytopathologica Sinica, 2022, 52(2): 235-246. (in Chinese)

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时间 Time	孢子浓度Spore concentration (spores/m³)								病情指数 Disease index
时间 Time	0：00	3：00	6：00	9：00	12：00	15：00	18：00	21：00	病情指数 Disease index
春（2021年5月） Spring (2021-05)	823±44	971±171	3309±531	12943±763	23368±159	11016±2	3335±213	1723±579	43.20
夏（2021年8月） Summer (2021-08)	779±46	1001±201	55629±853	72313±823	110697±685	82148±172	69252±150	33653±135	59.82
秋（2021年11月） Autumn (2021-11)	823±48	1201±180	16880 ±994	46883±910	64882±990	31170±991	4755±908	2378±780	53.85
冬（2022年2月） Winter (2022-02)	780±50	960±45	1683±835	5600±667	12445±774	1804±541	861±29	31±5	39.46

药剂 Fungicide	施药方式 Application method	施药时间 Application time	病情指数 Disease index	防治效果 Control efficiency (%)	孢子浓度 Spore concentration (spores/m³)	孢子灭杀效果 Spore killing efficiency (%)
60%多菌灵·乙霉威可湿性粉剂 Carbendazim + Diethofencarb 60% WP	弥粉法施药 Powder spraying	8：00	25.08	63.45±5.28c	4727±529	71.81c
		16：00	17.40	74.64±8.78b	4111±110	75.48b
		19：00	13.31	80.60±4.22a	2662±268	84.12a
	喷雾法施药 Water spraying	8：00	40.08	41.58±4.61d	7023±435	58.12d
		16：00	39.62	42.26±3.58d	7485±336	55.36de
		19：00	38.80	43.45±4.24d	7961±1068	52.52e
5亿孢子/g荧光假单胞杆菌可湿性粉剂 Pseudomonas fluorescens 500 million spores/g WP	弥粉法施药 Powder spraying	8：00	28.04	59.13±1.99c	4648±60	72.28c
		16：00	23.23	66.14±2.50b	3575±160	78.68b
		19：00	17.10	75.08±2.74a	2382±670	85.80a
	喷雾法施药 Water spraying	8：00	42.06	38.69±9.54d	5900±448	64.81d
		16：00	40.71	40.67±8.50d	5602±477	66.59d
		19：00	40.10	41.55±5.05d	4941±272	70.53cd
不施药对照Control	—	—	68.61	—	16768±2640	—

Effect of Humidity on Sporulation and Release of Corynespora cassiicola and Control Technology

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