Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3909-3920.doi: 10.3864/j.issn.0578-1752.2012.19.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Identification of QTLs for Major Isoflavone Components mong Multiple Environments in Soybean Seeds

 ZHANG  Jing-Ying, GE  Yi-Nan, SUN  Jun-Ming, HAN  Fen-Xia, YU  Fu-Kuan, YAN  Shu-Rong, YANG  Hua   

  1. 1.中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室 北京 100081
  • Received:2012-06-11 Online:2012-10-01 Published:2012-07-31

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Abstract: 【Objective】The QTLs for major isoflavone components were identified among multiple environments by different QTL mapping methods in soybean seeds, in order to provide a theoretical basis for soybean isoflavone marker-assisted selection. 【Method】 In this study, a recombinant inbreed line population (RIL, F5∶7-8) was developed by using the cross between the isoflavone contrasting cv. LHD2 with high-isoflavone concentration (3 697.24 μg•g-1) and cv. NHZ with low-isoflavone concentration (1 816.67 μg•g-1). The polymorphism of SSR markers among the RIL population was analyzed and the major isoflavone components were determined by HPLC. 【Result】The results showed that a soybean linkage map with a total distance of 3 546.54 cM was constructed using 161 polymorphism SSR molecular markers in the RIL population. A total of 14 QTLs associated with the major isoflavone components were found by ICIM, IM, and SMA methods in the ICIMapping 3.2 software under the four environments. 【Conclusion】The three QTLs flanked by the marker interval Sat_003-Satt306, Satt070-Satt122 and Satt571-Satt270 were detected among multiple environments in more than two methods.

Key words: soybean (Glycine max L. Merrill), isoflavone component, SSR, QTL

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