Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 3899-3908.doi: 10.3864/j.issn.0578-1752.2012.19.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Screening and Identification of Susceptibility and Fertility Related Mutants from Transgenic Rice IRBB13 (PXa13:GFP)

 HAN  Kuan-De, KONG  Ling-Guang, JU  Yan-Hu, CHEN  Ming, CAO  Zhi-Gang, DING  Xin-Hua, CHU  Zhao-Hui   

  1. 1.山东农业大学植物保护学院/作物生物学国家重点实验室/山东省农业微生物重点实验室，山东泰安 271018
    2.黄冈市农业科学院水稻研究所，湖北黄冈 438000
  • Received:2012-05-21 Online:2012-10-01 Published:2012-07-12

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Abstract: 【Objective】The objective of this study is to establish a simple and efficient mode of rice mutants selection, and lay the first stone for cloning rice genes involved in regulating xa13/ Xa13 gene expression or their mediated disease resistant /susceptible and pollen development signal pathway. 【Method】Using EMS treatment, a rice mutation population was constructed with IRBB13 (PXa13:GFP) which carrying green fluorescent protein(GFP) derived with dominant Xa13 gene promoter. Then the mutants were screened out with the following three periods: At seedling stage, selecting mutants in which GFP gene expression decline either in root or bud or non-expression; At adult stage, selecting mutants restored susceptible to PXO99; At booting stage, selecting mutants reduced fertility. 【Result】In total 3 000 M2 lines, 14 and 9 mutants were identified for both root and bud fluorescent declined and fluorescent normal only in root at seedling stage. At adult stage, relying on phenotype of PXO99 inoculation, a total of 25 lines were characterized to restore the susceptibility to PXO99. At booting stage, 7 mutants were identified, of which fertility declined and the green fluorescence of the anthers also disappeared and one mutant was found to restore susceptibility.【Conclusion】A simple and efficient mode of rice mutants selection system was established. Combining the abnormal expression pattern of GFP mutants, restored susceptible phenotype mutants and reduced fertility mutants, it was preliminarily indicated that the two signal transduction pathways mediated by xa13/ Xa13 genes are not only completely independent but also partially crossing between resistance/susceptibility and pollen development. Furthermore, after analysis and cloned the genes from these mutants, they will help us better understanding the functional mechanism of xa13/ Xa13 genes.

Key words: rice, EMS mutants, GFP, pollen development, bacterial blight

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