Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3551-3557.doi: 10.3864/j.issn.0578-1752.2012.17.011

• HORTICULTURE·STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Development and Mapping of Molecular Markers Closely Linked to CRb Gene Resistance to Clubroot Disease in Chinese Cabbage

 CHEN  Hui-Hui, ZHANG  Teng, LIANG  Shan, CHEN  Bing, ZHANG  Chun-Yu, PU  Zhong-Yun   

  1. 1.沈阳农业大学园艺学院，沈阳 110866
    2.华中农业大学作物遗传改良国家重点实验室/植物科学与技术学院，武汉 430070
  • Received:2012-04-06 Online:2012-09-01 Published:2012-06-29

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Abstract: 【Objective】The objective of this study is to develop molecular markers closely linked to clubroot resistance (CR) gene CRb in Chinese cabbage, and to evaluate their utility in germplasms of Brasisca rapa.【Method】A F2 mapping population was constructed with a cross between ‘CR Shinkii DH’ line, a Chinese cabbage doubled haploid line containing CRb gene, and a clubroot susceptible Chinese cabbage inbred line ‘07Q69’. Based on the target sequences showing homologous to the flanking markers TCR01, TCR05 and TCR09 linked to CRb on the chromosome A3 of Brassica rapa, primer pairs were designed and markers closely linked to CRb were developed. Forty-two germplasms belonging to different subspecies of B. rapa were employed to evaluate the transferability of markers linked to CRb.【Result】 Four co dominant and one dominant markers closely linked to CRb were developed and mapped. The markers TCR25 and TCR74 were located on one side of CRb, while TCR13, TCR42 and TCR34 were on the other side along with the previous markers TCR01 and TCR05. The CRb gene was flanked by two nearest markers TCR74 and TCR13 with the same genetic distance of 0.09 cM. The transferability evaluation of TCR74 and TCR13 showed that they possessed high polymorphism between clubroot resistant and susceptible sources.【Conclusion】The CRb gene was mapped between two codominant flanking markers with genetic distance of 0.18 cM. The two nearest flanking markers showed high polymorphism and good versatility between resistant and susceptible sources of B. rapa.  

Key words: Chinese cabbage, clubroot disease, CRb gene, molecular marker

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