Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4190-4198.doi: 10.3864/j.issn.0578-1752.2011.20.007

• PLANT PROTECTION • Previous Articles     Next Articles

Genetic Diversity of Phytophthora sojae in China Based on RFLP

 XIAO  Jiang-Tao, MIAO  Miao, GAO  Kun, XIANG  Gui-Lin, YANG  Shuai, DONG  Sha-Meng, WANG  Yuan-Chao, WANG  Ke-Rong   

  1. 1.南京农业大学农业部病虫监测与治理重点开放实验室
  • Received:2011-01-24 Online:2011-10-15 Published:2011-03-29

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Abstract: 【Objective】 The objective of this study is to explore the genetic diversity among Phytophthora sojae strains from soybean-growing regions in China and America. 【Method】 Restriction fragment length polymorphism (RFLP) was used to examine the genetic relationships among strains of P. sojae collected from 5 different regions. Nei’s genetic distances among the strains of P. sojae were calculated, and cluster analysis using UPGMA method was carried out by using the software package NTSYSpc V2.10. The Popgene V1.32 was used for calculation of genetic identity. 【Result】 RFLP probe PS127558 was selected and 25 fragments were detected, of which 24 was polymorphic, leading to the identification of 2 multilocus RFLP groups amongst the strains from Xinjiang and Inner Mongolia. RFLP group A which also exists in Heilongjiang occurred in over 88.2% of all samples collected from Xinjiang and 100% from Inner Mongolia, respectively. Genetic identity among populations of Xinjiang and Heilongjiang or Inner Mongolia and Heilongjiang is very high. Shannon’s information index revealed that the Heilongjiang populations have a higher genetic diversity than that of the Xinjiang and Inner Mongolia ones. RFLP probe PS127558 detected 17 fragments and 12 of which were polymorphic, among the 22 Fujian P. sojae strains, which lead to the distinction of 2 multilocus RFLP groups. Genetic identity is very low among the populations of Fujian and any of the other regions. The P. sojae strains between Heilongjiang and America show a high level of genetic identity, and several strains with shared DNA fingerprints were also found between Heilongjiang and America.【Conclusion】 P. sojae in Xinjiang and Inner Mongolia might have been introduced from Heilongjiang, and that in Fujian might belong to a separate evolutionary branch or originated from other area. In addition, exchange of P. sojae strains also occured between Heilongjiang and America.

Key words: Phytophthorarootrot, Phytophthorasojae, populationgenetics, origin, RFLP

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