青岛地区产ESBLs鸡源大肠杆菌耐药性调查与优势基因型分析

doi:10.3864/j.issn.0578-1752.2015.10.018

Abstract

Abstract: 【Objective】 By using antimicrobial susceptibility testing and detection of phenotype and genotype in extended spectrum beta-lactamases (ESBLs)， this study aims to find out the distribution and drug-resistance of ESBLs-producing E.coli strains on broiler farms in Qingdao, and then analyze genotype and gene subtype of ESBLs, so as to provide a basis for guiding rational clinical use of antimicrobial agents and effective control of infection and spread of ESBLs-producing strains.【Method】The antimicrobial susceptibility of 249 E. coli strains was determined by micro broth dilution, the phenotype of ESBLs was identified and determined by CLSI standard method, DNA was amplified and the genotype of drug resistance plasmid of ESBLs was analyzed by PCR, sequencing and biological software, and the significant difference between ESBLs-producing and non-producing strains was analyzed with SPSS19.0 software.【Result】There were 83.13% of E.coli strains produced ESBLs. The resistance rate of ESBLs-producing strains to 7 kinds of drugs was higher than that of non-ESBLs-producing ones, and there was a significant difference between them (P＜0.05) to GM, SPT, AM, A/C and CEF. But the resistance rate of ESBLs-producing strains to tetracycline and florfenicol was significantly lower than those in non-ESBLs-producing ones. The multi-drug resistance rate of ESBLs producing and non-producing strains was 99.03% and 92.86%, respectively (P=0.035). The detection rate of CTX-M, TEM and OXA genotype was 100%, 99.52% and 47.83%, respectively, and SHV genotype wasn’t detected. The enzyme producing strains belong to 10 gene subtypes, the dominant ones of which were TEM-1, CTX-M-65, CTX-M-55, and OXA-1. CTX-M-123 and CTX-M-64, the recombinant chimeras, were found from healthy poultry for the first time. 【Conclusion】The chicken origin ESBLs-producing E.coli strains are widely spread and transmitted in Qingdao. The antimicrobial resistance of ESBLs-producing strains are more serious than that of non-ESBLs-producing strains. Compared to other areas in China, CTX-M and TEM are also popular genotypes in Qingdao, but with different gene subtypes, TEM-1, CTX-M-65, CTX-M-55 and OXA-1 are dominant gene subtypes of each genotype, respectively.

Key words: chicken origin E.coli, drug-resistance, ESBLs, genotype, gene subtype

QU Zhi-na, LIU Hong-yu, WANG Juan, ZHAO Si-jun, LI Yu-qing, HUANG Xiu-mei, GAI Wen-yan, WANG Jun-wei. Antimicrobial Resistance Investigation and Dominant Genotype Analysis of ESBLs- Producing Escherichia coli Strains from Chicken in Qingdao[J].Scientia Agricultura Sinica, 2015, 48(10): 2058-2066.

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Antimicrobial Resistance Investigation and Dominant Genotype Analysis of ESBLs- Producing Escherichia coli Strains from Chicken in Qingdao

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