Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (15): 3193-3205.doi: 10.3864/j.issn.0578-1752.2011.15.015

• HORTICULTURE • Previous Articles     Next Articles

The Core-Collection Construction of Flower Lotus Based on AFLP Molecular Markers

YANG  Mei, FU  Jie, XIANG  Qiao-Yan, LIU  Yan-Ling   

  1. 1. 中国科学院武汉植物园水生植物与流域生态重点实验室
  • Received:2010-12-14 Revised:2011-01-11 Online:2011-08-01 Published:2011-05-24

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Abstract: 【Objective】 The construction of the core collection is very important for preservation, study and application of flower lotus (Nelumbo Adans.) germplasm resources. 【Method】 Based on AFLP markers, candidate core collections were constructed by using proportional strategy and UPGMA clustering sampling method within subgroups after dividing 395 flower lotus germplasm into 4 subgroups. By comparison of the genetic diversity parameters of these candidate core collection, such as the number of polymorphic loci, percentage of polymorphic loci, observed number of alleles, effective number of alleles, Nei’s gene diversity and Shannon’s information index, the core collection of flower lotus were screened, and genetic diversity comparison and t-test between original collection and core collection were analyzed. 【Result】 The core collection of flower lotus with 88 cultivars including 60 Chinese flower lotus (Nelumbo nucifera), 3 American louts (Nelumbo lutea), 16 hybrids between N. nucifera and N. lutea, and 9 Japanese lotus cultivars. The core collection reserved 22.27% samples of original collection. The reserved rate of number of polymorphic loci and percentage of polymorphic loci were 99.27%, and the reserved rate of observed number of alleles, effective number of alleles, Nei’s gene diversity and Shannon’s information index were 100.00%, 101.72%, 110.00% and 106.67%, respectively. t-test’s results showed that no significant difference was found in genetic diversity indexes between the core collection and original collection. 【Conclusion】 The core collection excluded the redundant germplasm from the original collection and conserved maximum genetic diversity with minimum lotus germplasm resource. These results demonstrated that the core collection could stand for original collection excellently.

Key words: Flower lotus, AFLP, Germplasm resource, Genetic diversity, Core collection

CLC Number: 

  • S682.32

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