Abstract:

【Objective】 The objective of this experiment was to study the regeneration technology of extensive adaptability of different rice varieties mature embryos. 【Method】 The mature embryos of different production types of rice were used as explants and the manifestations of tissue culture ability were studied comparatively, including callus induction frequency of mature embryo, differentiation rate and regeneration rate of 9 kinds of Japonica, 9 kinds of indica and 11 kinds of new cultivating super hybrid rice of two line or three line or superiority combinations in different media. 【Result】 When M8 was used as a basic medium, and 30 g•L-1 sucrose, 8 g•L-1 agar and 2 mg•L-1 2,4-D were added, single addition of cytokinin at lower concentration (0.3 mg•L-1 6-BA) in induction medium obtained higher callus induction frequency in Japonica between 61.7% and 89.2%. Based on addition of the two cytokinins (2 mg•L-1 6-BA and 0.5 mg•L-1 KT), further addition of 1 mg•L-1 NAA, the callus induction frequency of indica was improved obviously, between 9.1% and 100%. The callus induction frequency of 11 kinds of super hybrid rice were higher between 40% and 86.3% when addition of 1 mg•L-1 6-BA and 1 mg•L-1 KT, the cytokinins required by japonica and indica. On this basis adding 0.5 mg•L-1 2,4-D and 1 mg•L-1 6-BA (japonica), or 0.2 mg•L-1 2,4-D and 0.5 mg•L-1 6-BA (indica and hybrid rice) in the MS differentiation medium, the regeneration rates of plants reached 59.5%-9.2% (japonica), 87.5%-3.6% (indica), and 43.2%-17.2% (hybrid rice), respectively.【Conclusion】 The regeneration technology for higher frequency of the same type of rice varieties mature embryos was established preliminarily.

Key words: japonica')">japonica, indica, hybrid rice, mature embryos, hormone, tissue culture ability