Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (4): 782-787 .

• HORTICULTURE • Previous Articles     Next Articles

Physical Mapping of the 25S rDNA and 5S rDNA to Chinese Cabbage Metaphase Chromosome

  

  1. 河北农业大学园艺学院
  • Received:2006-02-27 Revised:1900-01-01 Online:2007-04-10 Published:2007-04-10

Abstract: With the help of fluorescene in situ hybridization(FISH), in the paper, we have located and analyzed the sites of the 25S and 5S rDNA on the mitotic metaphase chromosomes of Chinese cabbage(Brassica rapa ssp. Pekinensis). The results indicated that there were five pairs of signs detected for 25S rDNA, each at 1S, 2L, 3L, 4L and 10S(satellites) corresponding with the major characters of karyotype, and the signal intensity of the chromosomes were #10>#2>#3和#4>#1. The signal of 5S rDNA was located on 2L, 9S and 20S. in this study, the connection beween the signal location and the satellites and the heterochromatin was discussed.

Key words: Chinese Cabbage, 25S and 5S rDNA, FISH

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