Abstract: Effects of IGF-I, bFGF, TGF-α separation or combination on porcine oocytes maturation in vitro and cleavage after parthenogenetic activation were studied. The results showed that : (1)The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with 20 ng·ml-1 IGF-I is significantly higher compared with control and 10, 30, 40, 50, 100 ng·ml-1 groups[ (85.3 ±2.11)%, (85.4±2.81)%; (71.1 ±1.91)%, (62.5 ±0.98)%; (77.5 ±2.5)%, (59.1 ±3.93)%; (61.9 ±1.72)%, (54.3 ±3.48)%; (58.6 ±4.26)%, (53.1 ±1.23)%; (44.4 ±5.10)%, (49.8 ±3.55)%; (33.9 ±3.48)%, (46.1 ±3.59)%, P<0.05]，however when IGF-I solution arrive to 100 ng·ml-1, the maturation rate and the cleavage rate is greatly significant lower than other group (P<0.05)； (2)The maturation rate (85.0 ±1.70)% and the cleavage rate (85.0±2.82)% derived from oocytes matured in medium supplemented with 20 ng·ml-1 bFGF is greatly significant higher than other group (P<0.05)；(3) The maturation rate (86.9±0.46)% and the cleavage rate (86.3±2.01)% derived from oocytes matured in medium supplemented with 15 ng·ml-1 TGF-α is greatly significant higher than other group (P<0.05)；(4) The maturation rate and the cleavage rate derived from oocytes matured in medium supplemented with 15 ng·ml-1 TGF-α and 20 ng·ml-1 bFGF is significantly higher compared with 20 ng·ml-1 bFGF and 20 ng·ml-1 IGF-I group；15 ng·ml-1 TGF-α and 20 ng·ml-1 IGF-I group, 20 ng·ml-1 bFGF, 20 ng·ml-1 IGF-I and 15 ng·ml-1 TGF-α combation group.[(89.2±1.44)% and (88.8±0.17)%. (75.6±0.98)% and (78.3±1.65)% ; (77.2±2.54)% and (80.2±2.26)%; (76.4±1.28)% and (77.4±3.73)%, P<0.05].

Key words: IGF-I, bFGF, TGF-α, Pig, Oocyte, In vitro maturation, Oocytes cleavage