基于亲本对条锈病敏感性预测小麦杂交种的抗性

doi:10.3864/j.issn.0578-1752.2020.09.009

Abstract

Abstract:

【Objective】The objective of this study is to predict the resistance of wheat hybrids based on the resistance evaluation of their parental lines to stripe rust, and to enhance the predictability of disease resistance breeding in hybrid wheat. 【Method】A sensitive wheat variety, Mingxian 169, was used as negative control. A total of 13 restorer lines (male parents), 21 sterile lines (female parents), and their F₁ hybrids were inoculated with a suspension of a mixture of fresh urediniospores (equal amounts of stripe rust pathogen physiological races CYR23, CYR31, CYR33, and CYR34) in the field. Stripe rust resistance genes Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 were identified by PCR using their gene or linked molecular markers. In addition, semi-quantitative PCR was used to quantify the different stripe rust races in the adult plants of parent lines and a part of F₁ hybrids. 【Result】Yr5, Yr10 and Yr15 were not identified in all materials, Yr26 was mostly existed in Sichuan wheat lines, Yr9 and Yr17 were mostly existed in the northern lines, Yr18 was not identified in restorer lines. The parental resistance genes were polymerized in F₁ hybrids, which is consistent with the genetic law, indicating that the molecular marker can be used in wheat assistant breeding. The restorer lines from Sichuan and their F₁ hybrids showed high resistance against stripe rust, speculating that there are homozygous dominant resistance genes against stripe rust. Meanwhile, these wheat lines can be used for stripe rust resistance breeding in China. The infection types (ITs) of F₁ hybrids tended to the average of parental infection types. The results of binary regression analysis showed a significant correlation between parent lines and F₁ hybrids in ITs (R²=0.812). Although Yr5 and Yr15 resistance genes were absent in all tested wheat lines, the restorers from Sichuan Province and their F₁ hybrids showed higher resistance, speculating that those lines might carry unknown resistance genes against CYR34. The semi-quantitative PCR results indicated that CYR23 was not detected in all wheat lines. Only the restorer line 15CA50, sterile lines 17L6078 and 15L7128 were infected with a small amount of CYR31. The tested restorer lines Chuan 13 pin 6, MR1101, Chuanmai 98 and their F₁ hybrids were not infected by CYR33 or CYR34. Meanwhile, the parents with complementary resistance to different races were found to increase the resistance level of F₁ hybrids effectively. 【Conclusion】The F₁ hybrids resistance to strip rust can be predicted according to the average of the ITs of parental lines, the higher levels of disease resistance of parents, the better resistance of F₁ hybrids. Wheat varieties or lines with complementary resistance to different races of stripe rust should be screened as parents to improve the resistance level of F₁ hybrids. The results revealed the rule of disease resistance between parents and their F₁ hybrids, as well as providing a practical strategy for hybrid wheat disease resistance breeding.

Key words: hybrid wheat, stripe rust, resistance prediction, Puccinia striiformis f. sp. tritici, physiological race

TianYu ZHOU,JiangLing LI,Lan YANG,RenWu RUAN,YuHeng YANG,ZhongAn LI. The Resistance Prediction of Wheat Hybrids Based on the Sensibility of Their Parents to Stripe Rust[J].Scientia Agricultura Sinica, 2020, 53(9): 1806-1819.

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Figures/Tables 8

Table 1

Table 2

Fig. 1

Fig. 2

Table 3

Table 4

Disease resistance of F1 hybrids"

编号 Number	F₁代 F₁hybrids	反应型 Infection type	抗病评价 Resistance evaluation	Yr基因 Yr genes	亲本反应型平均值 Infection type (average of parents)
1	17L6065×小偃22 17L6065×Xiaoyan 22	5	MR	Yr9+Yr17	6
2	17L6067×小偃22 17L6067×Xiaoyan 22	5	MR	Yr9+Yr17	6
3	17L9160×小偃22 17L9160×Xiaoyan 22	5	MR	Yr9+Yr17	6
4	17L9154×陕987 17L9154×Shaan 987	4	MR	Yr9+Yr17	4.5
5	17L9160×陕987 17L9160×Shaan 987	4	MR	Yr9+Yr17	4
6	17L7123×高大1号 17L7123×Gaoda No.1	5	MR	Yr9+Yr17+Yr18	5
7	17L7140×高大1号 17L7140×Gaoda No.1	5	MR	Yr9+Yr17	5
8	17L7030×高大1号 17L7030×Gaoda No.1	7	MS	Yr9+Yr17+Yr18	6.5
9	17L6078×高大1号 17L6078×Gaoda No.1	6	MR	Yr9+Yr17	6.5
10	17L9066×高大1号 17L9066×Gaoda No.1	6	MR	Yr9+Yr17	6.5
11	17L9154×高大1号 17L9154×Gaoda No.1	6	MR	Yr9+Yr17	5.5
12	17L6067×丰德存5号 17L6067×Fengdecun No.5	6	MR	Yr9+Yr17	5
13	17L6019×丰德存5号 17L6019×Fengdecun No.5	5	MR	Yr9	6.5
14	17L6065×丰德存5号 17L6065×Fengdecun No.5	5	MR	Yr9+Yr17	5
15	17L7106×丰德存5号 17L7106×Fengdecun No.5	7	MS	Yr9+Yr18	5
16	17L7030×丰德存5号 17L7030×Fengdecun No.5	7	MS	Yr9+Yr17+Yr18	6.5
17	17L7123×丰德存5号 17L7123×Fengdecun No.5	7	MS	Yr9+Yr18	7
18	17L9154×丰德存5号 17L9154×Fengdecun No.5	5	MR	Yr9+Yr17	5.5
19	15L7109×丰德存5号 15L7109×Fengdecun No.5	6	MR	Yr9	5.5
20	17L6067×西农807 17L6067×Xinong 807	6	MR	Yr9+Yr17	5
21	17L6019×西农807 17L6019×Xinong 807	6	MR	Yr9	6.5
22	17L7030×西农807 17L7030×Xinong 807	5	MR	Yr9+Yr17+Yr18	6.5
23	17L7123×西农807 17L7123×Xinong 807	5	MR	Yr9+Yr18	5
24	17L7140×西农807 17L7140×Xinong 807	5	MR	Yr9	5
25	17L9154×西农807 17L9154×Xinong 807	6	MR	Yr9+Yr17	5.5
26	15L7109×西农807 15L7109×Xinong 807	5	MR	Yr9	5.5
27	17L6067×川14品16 17L6067×Chuan 14 pin 16	2	R	Yr9+Yr17+Yr26	2.5
28	15L7109×川14品16 15L7109×Chuan 14 pin 16	2	R	Yr9+Yr26	3
29	15L7128×川14品16 15L7128×Chuan 14 pin 16	2	R	Yr9+Yr26	4
30	15L7152×川14品16 15L7152×Chuan 14 pin 16	2	R	Yr9+Yr18+Yr26	2
31	17L6065×川13品6 17L6065×Chuan 13 pin 6	2	R	Yr9+Yr17+Yr26	3
32	17L6067×川13品6 17L6067×Chuan 13 pin 6	2	R	Yr9+Yr17+Yr26	3
33	17L9154×川13品6 17L9154×Chuan 13 pin 6	2	R	Yr9+Yr17+Yr26	3.5
34	17L6065×MR1101	2	R	Yr9+Yr17+Yr26	2.5
35	17L6067×MR1101	2	R	Yr9+Yr17+Yr26	2.5
36	17L6085×MR1101	2	R	Yr9+Yr17+Yr18+Yr26	3.5
37	17L7106×MR1101	2	R	Yr9+Yr26+Yr18	2.5
38	17L7030×MR1101	2	R	Yr9+Yr17+Yr26+Yr18	4
39	17L7123×MR1101	2	R	Yr9+Yr26+Yr18	2.5
40	17L7140×MR1101	2	R	Yr9+Yr26	2.5
41	17L9154×MR1101	0	R	Yr9+Yr17+Yr26	3
42	15L7109×MR1101	0	R	Yr9+Yr26	3
编号 Number	F₁代 F₁hybrids	反应型 Infection type	抗病评价 Resistance evaluation	Yr基因 Yr genes	亲本反应型平均值 Infection type (average of parents)
43	15L7128×MR1101	1	R	Yr9 +Yr26	4
44	15L7152×MR1101	2	R	Yr9+Yr18+Yr26	2
45	08L5070×MR1101	2	R	Yr9+Yr26	4
46	17L6065×MY13-3	2	R	Yr9+Yr17	2.5
47	17L6067×MY13-3	2	R	Yr9+Yr17	2.5
48	17L6085×MY13-3	2	R	Yr9+Yr17+Yr18	3.5
49	17L7106×MY13-3	2	R	Yr9+Yr17+Yr18	2.5
50	17L7030×MY13-3	2	R	Yr9+Yr17+Yr18	4
51	17L7123×MY13-3	2	R	Yr9+Yr17+Yr18	2.5
52	17L7140×MY13-3	2	R	Yr9+Yr17	2.5
53	17L9154×MY13-3	2	R	Yr9+Yr17	3
54	17L9163×MY13-3	2	R	Yr9+Yr17	4
55	15L7109×MY13-3	2	R	Yr9+Yr17	3
56	15L7128×MY13-3	2	R	Yr9+Yr17	4
57	15L7152×MY13-3	2	R	Yr9+Yr17+Yr18	2
58	08L5070×MY13-3	2	R	Yr9+Yr17	4
59	17L6065×川麦93 17L6065×Chuanmai 93	1	R	Yr9+Yr17+Yr26	1.5
60	17L6067×川麦93 17L6067×Chuanmai 93	1	R	Yr9+Yr17+Yr26	1.5
61	17L6085×川麦93 17L6085×Chuanmai 93	1	R	Yr9+Yr26	2.5
62	17L7106×川麦93 17L7106×Chuanmai 93	1	R	Yr9+Yr26+Yr18	1.5
63	17L7030×川麦93 17L7030×Chuanmai 93	2	R	Yr9+Yr17+Yr26+Yr18	3
64	17L7123×川麦93 17L7123×Chuanmai 93	1	R	Yr9+Yr26+Yr18	1.5
65	17L7140×川麦93 17L7140×Chuanmai 93	1	R	Yr9+Yr26	1.5
66	17L9154×川麦93 17L9154×Chuanmai 93	1	R	Yr9+Yr17+Yr26	2
67	17L9163×川麦93 17L9163×Chuanmai 93	2	R	Yr9+Yr26	3
68	15L7109×川麦93 15L7109×Chuanmai 93	2	R	Yr9+Yr26	2
69	15L7128×川麦93 15L7128×Chuanmai 93	2	R	Yr9+Yr26	3
70	15L7152×川麦93 15L7152×Chuanmai 93	2	R	Yr9+Yr18+Yr26	1
71	08L5070×川麦93 08L5070×Chuanmai 93	2	R	Yr9+Yr26	3
72	17L6065×川麦98 17L6065×Chuanmai 98	2	R	Yr9+Yr17+Yr26	1.5
73	17L6067×川麦98 17L6067×Chuanmai 98	2	R	Yr9+Yr17+Yr26	1.5
74	17L6085×川麦98 17L6085×Chuanmai 98	2	R	Yr9 +Yr17+Yr18+Yr26	2.5
75	17L7106×川麦98 17L7106×Chuanmai 98	2	R	Yr9+Yr18+Yr26	1.5
76	17L7030×川麦98 17L7030×Chuanmai 98	2	R	Yr9+Yr17+Yr18+Yr26	3
77	17L7123×川麦98 17L7123×Chuanmai 98	2	R	Yr9+Yr18+Yr26	3
78	17L7140×川麦98 17L7140×Chuanmai 98	2	R	Yr9+Yr26	1.5
79	17L9160×15CA50	6	MR	Yr9+Yr17	5
80	17L6062×15CA50	7	MS	Yr9	5
81	17L7106×15CA50	6	MR	Yr9+Yr18	5
82	17L7140×15CA50	5	MR	Yr9	5
83	17L9160×伟隆121 17L9160×Weilong 121	4	MR	Yr9+Yr17	3.5
84	17L6062×伟隆121 17L6062×Weilong 121	4	MR	Yr9+Yr17	3.5
85	17L6065×伟隆121 17L6065×Weilong 121	4	MR	Yr9+Yr17	3.5
86	17L7106×伟隆121 17L7106×Weilong 121	3	R	Yr9+Yr17+Yr18	3.5

Table 4

Fig. 3

Fig. 4

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Yr 基因 Yr genes	引物名称 Primer name	引物序列 Primer sequence (5′-3′)	退火温度 Anneal temperature (℃)	片段大小 Fragment size (bp)
Yr5	Yr5-InsertionF^[26] Yr5-InsertionR	CTCACGCATTTGACCATATACAACT TATTGCATAACATGGCCTCCAGT	52	+1281
Yr9	AF1^[27] AF4	F: GGAGACATCATGAAACATTTG R: CTGTTGTTGGGCAGAAAG	46	+1500
Yr10	Yr10F1^[27] Yr10R1	TTGGAATTGGCGACAAGCGT GTGATGATTACCCACTTCCTC	50	+755
Yr15	Y15K1_F2^[28] uhw301R	GGAGATAGAGCACATTACAGAC TTTCGCATCCCACCCTACTG	55	+992
	W_2F	TGCACGCGGATATTAGGTAGG	55	+2014
	W_2R	TGATGAAGAGGACCAACGCA
Yr17	VENTRIUP^[27] LN2	AGGGGCTACTGACCAAGGCT TGCAGCTACAGCAGTATGTACACAAAA	55	+262
Yr18	L34DINT9F^[27] L34PLUSR	TTGATGAAACCAGTTTTTTTTCTA GCCATTTAACATAATCATGATGGA	45	+517
Yr26	WE173F^[27] WE173R	GGGACAAGGGGAGTTGAAGC GAGAGTTCCAAGCAGAACAC	50	+451 -730

引物名称 Primer name		引物序列 Primer sequence (5′-3′)	退火温度 Anneal temperature (℃)
CYR23	S360^[33] S413	F: AAGCGGCCTC R: GGTGGTCCAAG	50
CYR31	CY31SP-1^[34] CY31SP-2	F: GCTACGTCAAGATGCGATACACC R: TGTCAGAAGCAAGTGGTAAACTAGG	50
CYR33	CYR33SP-1^[35] CYR33SP-2	F: TGTCGTCTCGCCAATCTTT R: GCGGGTGTCAGTTTCTCC	50
CYR34	V26SP-1^[36] V26SP-2	F: CTGTAAAGCGGATAAAGGAA R: CATAAGAGCCACACTTGACC	57
Pst elongation factor	Pst_EF	F: TTCGCCGTCCGTGATATGAGACAA R: ATGCGTATCATGGTGGTGGAGTGA	55

The Resistance Prediction of Wheat Hybrids Based on the Sensibility of Their Parents to Stripe Rust

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	品系 Line	反应型 Infection type	抗病评价 Resistance evaluation	Yr 基因 Yr genes
恢复系 Restorer lines	小偃22 Xiaoyan 22	9	S	Yr9
	陕987 Shaan 987	5	MR	Yr9+Yr17
	高大1号 Gaoda No.1	7	MS	Yr9+Yr17
	伟隆121 Weilong 121	4	MR	Yr17
	15CA50	7	MS	-
	丰德存5号 Fengdecun No.5	7	MS	-
	西农807 Xinong 807	7	MS	-
	川14品16 Chuan 14 pin 16	2	R	Yr26
	川13品6 Chuan 13 pin 6	2	R	Yr26
	川麦93 Chuanmai 93	0	R	Yr26
	川麦98 Chuanmai 98	0	R	Yr17+Yr26
	MR1101	2	R	Yr26
	MY13-3	2	R	Yr17
不育系 Sterile lines	08L5070	6	MR	Yr9
	15L7109	4	MR	Yr9
	15L7128	6	MR	Yr9
	15L7152	2	R	Yr9+Yr18
	17L6019	6	MR	Yr9
	17L6062	3	R	Yr9
	17L6065	3	R	Yr9+Yr17
	17L6067	3	R	Yr9+Yr17
	17L6078	6	MR	Yr9
	17L6085	5	MR	Yr9+Yr17+Yr18
	17L7030	6	MR	Yr9+Yr17+Yr18
	17L7106	3	R	Yr9+Yr18
	17L7123	3	R	Yr9+Yr18
	17L7140	3	R	Yr9
	17L9066	6	MR	-
	17L9154	4	MR	Yr9+Yr17
	17L9160	3	R	Yr9+Yr17
	17L9163	6	MR	Yr9
	17L9195	3	R	Yr9
	17L9210	2	R	Yr9+Yr17
	17L9217	4	MR	Yr9

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