Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 3919-3929.doi: 10.3864/j.issn.0578-1752.2011.19.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Genetic Diversity and Genome-Wide Association Analysis of Stripe Rust Resistance Among the Core Wheat Parent Zhou 8425B and Its Derivatives

 XIAO  Yong-Gui, YIN  Gui-Hong, LI  Hui-Hui, XIA  Xian-Chun, YAN  Jun, ZHENG  Tian-Cun, JI  Wan-Quan, HE  Zhong-Hu   

  1. 1.西北农林科技大学农学院
    2.中国农业科学院作物科学研究所/国家小麦改良中心
    3.周口市农业科学院
    4.中国农业科学院棉花研究所
  • Received:2011-03-23 Online:2011-10-01 Published:2011-05-19

PDF

1350

Cited

26

Abstract: 【Objective】 Zhou 8425B is the most important core parent and has been widely used in the South Part of the Yellow and Huai Valleys Winter Wheat Zone. Understanding the principal components and detecting the wheat stripe rust resistance loci in Zhou 8425B will provide important information for the future genetic improvement of wheat varieties. 【Method】 The core parent Zhou 8425B and its 50 derivative varieties and lines were used to compare their genetic structure and components among different generations. Whole-genome association mapping was employed to identify the stripe rust resistance loci using 921 Diversity Array Technology (DArT) and 83 SSR markers. 【Result】The averaged genetic similarity index was 67.6% among Zhou 8425B and its derivatives, and the clustering results for these genotypes were basically in consistent with that from the pedigree analysis. On the whole-genome levels, Zhou 8425B had the contribution ratios of 67.7%, 63.6% and 58.8% to its first, second and third generation derivatives, respectively. The genetic contribution ratios of A, B and D subgenome levels were 68.7%, 62.0% and 59.4%, respectively. As for single chromosome level, 44.9% (4A) to 70.9% (1D) of Zhou 8425B alleles could be detected on the derivative varieties. Four loci were detected to be associated with stripe rust resistance by association mapping (P＜0.01), in which two loci QYr.caas-2BL and QYr.caas-7BL were found in the same genomic regions of Yr7 and YrZH84, respectively. A QTL QYr.caas-1BL was found in the same genomic region as leaf rust resistance gene LrZH84 reported previously, which might be attributed to be pleiotropic effect or tight linkage each other. A new locus QYr.caas-3AL was located on the long arm of chromosome 3A and linked with the terminal marker wPt-0398, explaining 22.9% of the phenotypic variance.【Conclusion】The contribution ratios transferred from the core parent to its derivatives on genome and chromosome levels were probably caused by various important alleles associated with agronomic traits and resistance genes. Four QTLs for stripe rust resistance detected in 50 derivative varieties were derived from the core parent Zhou 8425B. Therefore, it is presumed that these loci for stripe rust resistance and other important traits derived from Zhou 8425B could play important roles in the future wheat breeding program in China, especially in the South Part of the Yellow and Huai Valleys Winter Wheat Zone.

Key words:

[1]金善宝. 中国小麦品种及其系谱. 北京: 农业出版社, 1983.

Jin S B. Wheat Varieties and Their Pedigrees in China. Beijing: Agriculture Press, 1983. (in Chinese)

[2]庄巧生. 中国小麦品种改良及系谱分析. 北京: 中国农业出版社, 2003: 10-169.

Zhuang Q S. Chinese Wheat Improvement and Pedigree Analysis. Beijing: China Agricultural Press, 2003: 10-169. (in Chinese)

[3]李晴祺. 冬小麦种质创新与评价利用. 济南: 山东科学技术出版社, 1998.

Li Q Q. Creation, Evaluation and Utilization of Winter Wheat Germplasm. Jinan: Shandong Science and Technology Press, 1998. (in Chinese)

[4]殷贵鸿, 王建武, 闻伟锷, 何中虎, 李在峰, 王  辉, 夏先春. 小麦抗条锈病基因YrZH84的RGAP标记及其应用. 作物学报, 2009, 35: 1274-1281.

Yin G H, Wang J W, Wen W E, He Z H, Li Z F, Wang H, Xia X C. Mapping of wheat stripe rust resistance gene YrZH84 with RGAP markers and its application. Acta Agronomica Sinica, 2009, 35: 1274-1281. (in Chinese)

[5]Li Z F, Zheng T C, He Z H, Li G Q, Xu S C, Li X P, Yang G Y, Singh R P, Xia X C. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 2006, 112: 1098-1103.

[6]Zhao X L, Zheng T C, Xia X C, He Z H, Liu D Q, Yang W X, Yin G H, Li Z F. Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 2008, 117: 1069-1075.

[7]Thornsberry J M, Goodman M M, Doebley J, Kresovich S, Nielsen D, Buckler E S. Dwarf8 polymorphisms associate with variation in flowering time. Nature Genetics, 2001, 28: 286-289.

[8]Breseghello F, Sorrells M E. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics, 2006, 172: 1165-1177.

[9]Breseghello F, Sorrells M E. QTL analysis of kernel size and shape in two hexaploid wheat mapping populations. Field Crops Research, 2007, 101: 172-179.

[10]张学勇, 童依平, 游光霞, 郝晨阳, 盖红梅, 王兰芬, 李  滨, 董玉琛, 李振声. 选择牵连效应分析: 发掘重要基因的新思路. 中国农业科学, 2006, 39: 1526-1535.

Zhang X Y, Tong Y P, You G X, Hao C Y, Gai H M, Wang L F, Li B, Dong Y C, Li Z S. Hitchhiking effect mapping: A new approach for discovering agronomic important genes. Scientia Agricultura Sinica, 2006, 39: 1526-1535. (in Chinese)

[11]Crossa J, Burgueño J, Dreisigacker S, Vargas M, Herrera-Foessel S A, Lillemo M, Singh R P, Trethowan R, Warburton M, Franco J, Reynolds M, Crouch J H, Ortiz R. Association analysis of historical bread wheat germplasm using additive genetic covariance of relatives and population structure. Genetics, 2007, 177: 1889-1913.

[12]韩  俊, 张连松, 李静婷, 石丽娟, 解超杰, 尤明山, 杨作民, 刘广田, 孙其信, 刘志勇. 小麦骨干亲本“胜利麦/燕大1817”杂交组合后代衍生品种遗传构成解析. 作物学报, 2009, 35: 1395-1404.

Han J, Zhang L S, Li J T, Shi L J, Xie C J, You M S, Yang Z M, Liu G T, Sun Q X, Liu Z Y. Molecular dissection of core parental cross “Triumph/Yanda 1817” and its derivatives in wheat breeding program. Acta Agronomica Sinica, 2009, 35: 1395-1404. (in Chinese)

[13]袁园园, 王庆专, 崔  法, 张景涛, 杜  斌, 王洪刚. 小麦骨干亲本碧蚂4号的基因组特异位点及其在衍生后代中的传递. 作物学报, 2010, 36: 9-16.

Yuan Y Y, Wang Q Z, Cui F, Zhang J T, Du B, Wang H G. Specific loci in genome of wheat milestone parent Bima 4 and their transmission in derivatives. Acta Agronomica Sinica, 2010, 36: 9-16. (in Chinese)

[14]李小军, 徐  鑫, 刘伟华, 李秀全, 李立会. 利用SSR标记探讨骨干亲本欧柔在衍生品种的遗传. 中国农业科学, 2009, 42: 3397-3404.

Li X J, Xu X, Liu W H, Li X Q, Li L H. Genetic diversity of the founder parent Orofen and its progenies revealed by SSR markers. Scientia Agricultura Sinica, 2009, 42: 3397-3404. (in Chinese)

[15]Somers D J, Isaac P, Edwards K. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2004, 109: 1105-1114.

[16]Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology, 2005, 14: 2611-2620.

[17]Christopher M, Mace E, Jordan D, Rodgers D, McGowan P, Delacy I, Banks P, Sheppard J, Butler D, Poulsen D. Applications of pedigree-based genome mapping in wheat and barley breeding programs. Euphytica, 2007, 154: 307-316.

[18]Flint-Garcia S A, Thornsberry J M, Buckler E S. Structure of linkage disequilibrium in plants. Annual Review of Plant Biology, 2003, 54: 357-374.

[19]Kilian A, Huttner E, Wenzi P, Jaccoud D, Carling J, Caig V, Evers M, Heller-Uszynska K, Uszynski G, Cayla C, Patarapuwadol S, Xia L, Yang S, Thomson B. The fast and the cheap: SNP and DArT-based whole genome profiling for crop improvement//Tuberosa R, Phillips R L, Gale M. Proceedings of the International Congress “In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution”. Bologna, Italy, 2003: 443-461.

[20]Somers D J, Banks T, DePauw R, Fox S, Clarke J, Pozniak C, McCartney C. Genome-wide linkage disequilibrium analysis in bread wheat and durum wheat. Genome, 2007, 50: 557-567.

[21]郝晨阳, 王兰芬, 张学勇, 游光霞, 董玉琛, 贾继增, 刘  旭, 尚勋武, 刘三才, 曹永生. 我国育成小麦品种的遗传多样性演变. 中国科学: C辑, 2005, 35: 408-415.

Hao C Y, Wang L F, Zhang X Y, You G X, Dong Y C, Jia J Z, Liu X, Shang X W, Liu S C, Cao Y S. Genetic diversity in Chinese modern wheat varieties revealed by microsatellite markers. Science in China: Series C, 2005, 35: 408-415. (in Chinese)

[22]Pestsova E, Ganal M W, Röder M S. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 2000, 43: 689-697.

[23]何中虎, 兰彩霞, 陈新民, 邹裕春, 庄巧生, 夏先春. 小麦条锈病和白粉病成株抗性研究进展与展望. 中国农业科学, 2011, 44(11): 2193-2215.

He Z H, Lan C X, Chen X M, Zou Y C, Zhuang Q S, Xia X C. Adult- plant resistance to stripe rust and powdery mildew in wheat, progress and perspective. Scientia Agricultura Sinica, 2011, 44(11): 2193-2215. (in Chinese)

[24]Mallard S, Gaudet D, Aldeia A, Abelard C, Besnard A L, Sourdille P, Dedryver F. Genetic analysis of durable resistance to yellow rust in bread wheat. Theoretical and Applied Genetics, 2005, 110: 1401-1409.

[25]Boukhatem N, Baret P V, Mingeot D, Jacquemin J M. Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theoretical and Applied Genetics, 2002, 104: 111-118.
[1] NIE XingHua, ZHENG RuiJie, ZHAO YongLian, CAO QingQin, QIN Ling, XING Yu. Genetic Diversity Evaluation of Castanea in China Based on Fluorescently Labeled SSR [J]. Scientia Agricultura Sinica, 2021, 54(8): 1739-1750.
[2] GAO Yuan,WANG DaJiang,WANG Kun,CONG PeiHua,LI LianWen,PIAO JiCheng. Genetic Relationship and Structure Analysis of 15 Species of Malus Mill. Based on SNP Markers [J]. Scientia Agricultura Sinica, 2020, 53(16): 3333-3343.
[3] SHI TianTian, HE JieLi, GAO ZhiJun, CHEN Ling, WANG HaiGang, QIAO ZhiJun, WANG RuiYun. Genetic Diversity of Common Millet Resources Assessed with EST-SSR Markers [J]. Scientia Agricultura Sinica, 2019, 52(22): 4100-4109.
[4] ZHANG HuaiShan, YAN XiuQin, LU Min, WANG DaoPing, AN HuaMing. Analysis of the Genetic Diversity of Wild Rosa roxburghii Populations in Guizhou Province Based on EST-SSR Marker [J]. Scientia Agricultura Sinica, 2017, 50(6): 1098-1108.
[5] QU Yang, ZHOU Yu, WANG Zhao, WANG Peng-ke, GAO Jin-feng, GAO Xiao-li, FENG Bai-li . Analysis of Genetic Diversity and Structure of Tartary Buckwheat Resources from Production Regions [J]. Scientia Agricultura Sinica, 2016, 49(11): 2049-2062.
[6] ZHANG Qiu-Ping, LIU Dong-Cheng, LIU Wei-Sheng, LIU Shuo, ZHANG Ai-Min, LIU Ning, ZHANG Yu-Ping. Genetic Diversity and Population Structure of the North China Populations of Apricot (Prunus armeniaca L.) [J]. Scientia Agricultura Sinica, 2013, 46(1): 89-98.
[7] ZHANG Li-Na, CAO Gui-Lan, HAN Long-Zhi. Analysis of Genetic Diversity of Japonica Rice Landrace in China with Microsatellite Marker [J]. Scientia Agricultura Sinica, 2012, 45(3): 405-413.
[8] QIAO Xiao-Yan, QIAO Ting-Ting, ZHOU Yan-Hua, JIN Ji-Qiang, MA Chun-Lei, YAO Ming-Zhe, CHEN Liang. Comparative Analysis of Genetic Structure and Differentiation of Guangdong and Guangxi Tea Germplasms Based on EST-SSR Markers [J]. Scientia Agricultura Sinica, 2011, 44(16): 3297-3311.
[9] ZHANG Jun,ZHAO Tuan-jie,GAI Jun-yi. Analysis of Genetic Structure Differentiation of Released Soybean Cultivar Population and Specificity of Subpopulations in China
 [J]. Scientia Agricultura Sinica, 2009, 42(6): 1901-1910 .
[10] Yong-Chun LI. The Natural Selection Effect on Major Quantitative Characters in RILs Population Derived from The Same Soybean Hybrid Combination Under Different Enviroments. [J]. Scientia Agricultura Sinica, 2008, 41(7): 1917-1926 .
[11] . Study on genetic structure of Native Sheep Populations in East and South Asia [J]. Scientia Agricultura Sinica, 2007, 40(8): 1786-1794 .
[12] ,,. Molecular Genetic Studies on the Rice Blast Fungus Population-- Dynamics of Genetic and Pathotypic Structures of Three Rice Blast Fungus Populations Originated from Guangdong Province in 2000 to 2002 [J]. Scientia Agricultura Sinica, 2004, 37(10): 1468-1473 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd