Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3240-3248.doi: 10.3864/j.issn.0578-1752.2012.16.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Analysis of Wheat-Thinopyrum ponticum Translocation Lines with Broad Spectrum Resistance to Stripe Rusts

 HAO  Wei-Wei, TANG  Cai-Guo, LI  Bao-Chun, HAO  Chen-Yang, ZHANG  Xue-Yong   

  1. 1.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物基因资源与种质创制重点开放实验室，北京 100081
    2.南京农业大学农学院，南京 210095；3甘肃农业大学农学院，兰州 730000
  • Received:2012-03-21 Online:2012-08-15 Published:2012-06-19

PDF

1399

Cited

5

Abstract: 【Objective】To transfer strip rust resistance from Thinopyrum ponticum to common wheat, wide hybrid progenies between them were screened for strip rust resistance.【Method】The progenies were characterized by their disease resistance，agronomic traits，genomic in situ hybridization and quality related protein analysis.【Result】 Eight lines with good resistance or immune to the pathogens at adult stage were obtained. All of them have 42 chromosomes, in which seven lines convey a new translocation, 4DS/4St. In addition, besides this translocation, the GDR3 conveys another terminal translocation, which may be derived from the E-genome chromosomes of Th. ponticum. The GDR3 performed very good resistance in whole life. It was immune to yellow rusts. It was estimated that both of the alien segments convey resistant genes to the stripe rust. The high molecular weight glutenin subunits (HMW-GS) analysis indicated that all of the eight lines convey the good subunits 14+15 at Glu-B1, which should be inherited from Xiaoyan 6. 【Conclusion】 Wild relatives of wheat usually convey genes with broad spectrum resistance to pathogens. This is the genetic base for their surviving in extreme environment, which also provide excellent gene resource for disease resistant breeding in wheat.

Key words: common wheat, Thinopyrum ponticum, stripe rust, translocation

[1] Roelfs A P, Singh R P, Saari E E. Rust diseases of wheat: Concepts and methods of disease management. CIMMYT, Mexico, 1992: 14-15.

[2] McIntosh R A, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers D J, Appels R, Devos K M. Catalogue of gene symbols for wheat// Proc 11th Int Wheat Genet Symp. University of Sydney Press, Australia, 2008: 24-29.

[3] Jiang J, Friebe B, Gill B S. Recent advances in alien gene transfer in wheat. Euphytica, 1994, 73: 199-212.

[4] 李振声. 我国小麦育种的回顾与展望. 中国农业科技导报, 2010, 12(2): 1-4.

Li Z S. Retrospect and prospect of wheat breeding in China.   Journal of Agricultural Science and Technology, 2010, 12(2): 1-4. (in Chinese)

[5] Zhang X Y, Dong Y S, Wang R R C. Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum ? Thinopyrum ponticum by in situ hybridization, isozyme analysis and RAPD. Genome, 1996, 39(6): 1062-1071.

[6] Li H J, Wang X M. Thinopyrum ponticum and Th. intermedium. the promising source of resistance to fungal and viral diseases of wheat. Journal of Genetics and Genomics, 2009, 36: 557-565.

[7] 张荣琦, 陈春环, 赵晓农, 钟冠昌. 利用远缘杂交技术选育小麦新品种之研究. 中国农学通报, 2006, 22(6): 186-188.

Zhang R Q, Chen C H, Zhao X N, Zhong G C. The study on selection of wheat varieties with distant hybrid technique. Chinese Agricultural Science Bulletin, 2006, 22(6): 186-188. (in Chinese)

[8] 夏光敏, 向凤宁, 周爱芬, 王  槐, 何世贤, 陈惠民. 小麦与高冰草的不对称体细胞杂交获可育杂种植株. 植物学报, 1999, 41(4): 349-352.

Xia G M, Xiang F N, Zhou A F, Wang H, He S X, Chen H M. Fertile hybrid plant regeneration from somatic hybridization between Triticum aestivum and Agropyron elongatum. Acta Botanica Sinica, 1999, 41(4): 349-352. (in Chinese)

[9] 殷学贵, 尚勋武, 庞斌双, 宋建荣, 曹世勤, 李金昌, 张学勇. A-3中抗条锈新基因YrTp1和YrTp2的分子标记定位分析. 中国农业科学, 2006, 39(1): 10-17.

Yin X G, Shang X W, Pang B S, Song J R, Cao S Q, Li J C, Zhang X Y. Molecular mapping of two novel stripe rust resistance genes YrTp1 and YrTp2 in A-3 drived from Triticum aestivum×Thinopyrum ponticum. Scientia Agricultura Sinica, 2006, 39(1): 10-17. (in Chinese)

[10] Payne P I, Harris P A, Law C N, Holt L M, Blackman J A. The high-molecular-weight subunits of glutenin: Structure, genetics and relationship to bread-making quality. Annual Technology Agricultural, 1980, 29: 309-320.

[11] Weegles P L, Hamer R J, Schofield J D. Functional properties of wheat glutenin. Journal of Cereal Science, 1996, 23: 1-18.

[12] 蔡民华, 胡英考, 李雅轩, 李建国, 晏月明. 小麦谷蛋白亚基对品质性状的影响. 首都师范大学学报, 2006, 27(3): 61-66.

Cai M H, Hu Y K, Li Y X, Li J G, Yan Y M. Effects of common wheat quality traits affected by glutenin subunits. Journal of Capital Normal University, 2006, 27(3): 61-66. (in Chinese)

[13] Liu Z, Yue W, Dong Y S, Zhang X Y. Identification and preliminary analysis of several centromere-associated bacterial artificial chromosome clones from a diploid wheat library. Journal of Integrative Plant Biology, 2006, 48(3): 348-358.

[14] Rayburn A L, Gill B S. Molecular identification of the D-genome chromosomes of wheat. Journal of Heredity, 1986, 77: 253-255.

[15] Mukai Y, Nakahara Y, Yamamoto M. Simultaneous discrimination of the three genomes in hexaploid wheat by multi-color fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 1993, 36: 489-494.

[16] Chai J F, Zhou R H, Jia J Z, Liu X. Development and application of a new codominant PCR marker for detecting 1BL?1RS wheat-rye chromosome translocations. Plant Breeding, 2006, 125: 302-304.

[17] 张学勇, 庞斌双, 游光霞, 王兰芬, 贾继增, 董玉琛. 中国小麦品种资源Glu-1位点组成概况及遗传多样性分析. 中国农业科学, 2002, 35(11): 1302-1310.

Zhang X Y, Pang B S, You G X, Wang L F, Jia J Z, Dong Y C. Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Scientia Agricultura Sinica, 2002, 35(11): 1302-1310. (in Chinese)

[18] Wang R R C, Zhang X Y. Characterization of the translocated chromosome using fluorescence in situ hybridization and random amplified polymorphic DNA on two Triticum aestivum-Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus. Chromosome Research, 1996, 4: 583-587.

[19] Liu Z, Li D Y, Zhang X Y. Genetic relationships among five basic genomes St, E, A, B and D in Triticeae revealed by genomic Southern and in situ hybridization. Journal of Integrative Plant Biology, 2007, 49(7): 1080-1086.

[20] Fedak G, Han F P. Characterization of derivatives from wheat-Thinopyrum wide crosses. Cytogenet Genome Research, 2005, 109: 360-367.

[21] Li H J, Wang X M. Thinopyrum ponticum and Th. intermedium: The promising source of resistance to fungal and viral diseases of wheat. Journal of Genetics and Genomics, 2009, 36: 557-565.

[22] Li Z S, Li B, Tong Y P. The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China. Journal of Genetics and Genomics, 2008, 35: 451-456.

[23] Zhang X Y, Li Z S, Chen S Y. Production and identification of three 4Ag (4D) substitution lines of Triticum aestivum-Agropyron: Relative transmission rate of alien chromosomes. Theoretical and Applied Genetics, 1992, 83: 707-714.

[24] 张学勇, 董玉琛, 栗站稳. 小麦和彭梯卡偃麦草杂种及其衍  生后代的细胞遗传学研究: Ⅰ彭梯卡偃麦草及其与普通小麦和硬粒小麦杂种F1的染色体配对. 遗传学报, 1993, 20(5): 439-447.

Zhang X Y, Dong Y C, Li Z W. Cytogenetic research on hybrids between Triticum and decaploid Thinopyrum ponticum and their derivatives: Ⅰ Chromosome pairing in decaploid Th. ponicum and F1 hybrids of it with both T.aestivum and T.durum. Acta Genetica Sinica, 1993, 20(5): 439-447. (in Chinese)

[25] 张学勇, 董玉琛, 杨欣明. 小麦与长穗偃麦草、中间偃麦草杂种及其衍生后代的细胞遗传学研究: Ⅲ 小麦和偃麦草基因重组的遗传基础浅析. 遗传学报, 1993, 22(3): 217-222.

Zhang X Y, Dong Y C, Yang X M. Cytogenetic research on hybrids of Triticum with both Thinopyrum ponticum and Th.intermedium as well as their derivatives: Ⅲ Primary detection of genetic base for introgression of useful genes from the two alien species to wheat. Acta Genetica Sinica, 1993, 22(3): 217-222. (in Chinese)

[26] Sanei M, Pickering R, Kumke K, Nasuda S, Houben A. Loss of centromeric histone H3  (CENH3) from centromeres precedes uniparental chromosome elimination in interspeci?c barley hybrids. Proceedings of the National Academy of Sciences of the USA, 2011, 108(33): 498-505.

[27] Friebe B, Jiang J, Raupp W J, Mclntosh R A, Gill B S. Characterization of wheat-alien translocations conferring resistance to diseases and pests: Current status. Euphytica, 1996, 91: 59-87.

[28] Han F P, Fedak G, Benabdelmouna A, Armstrong K, Ouellet        T. Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP and multicolor GISH. Genome, 2003, 46: 490-495.

[29] Qi L, Friebe B, Zhang P, Gill B S. Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Research, 2007, 15: 3-19.

[30] Chen G L, Zheng Q, Bao Y G. Molecular cytogenetic identification of a novel dwarf wheat line with introgressed Thinopyrum ponticum chromatin. Journal of Biosciences, 2012, 37(1): 149-155.
[1] TANG HuaPing,CHEN HuangXin,LI Cong,GOU LuLu,TAN Cui,MU Yang,TANG LiWei,LAN XiuJin,WEI YuMing,MA Jian. Unconditional and Conditional QTL Analysis of Wheat Spike Length in Common Wheat Based on 55K SNP Array [J]. Scientia Agricultura Sinica, 2022, 55(8): 1492-1502.
[2] HU ChaoYue, WANG FengTao, LANG XiaoWei, FENG Jing, LI JunKai, LIN RuiMing, YAO XiaoBo. Resistance Analyses on Wheat Stripe Rust Resistance Genes to the Predominant Races of Puccinia striiformis f. sp. tritici in China [J]. Scientia Agricultura Sinica, 2022, 55(3): 491-502.
[3] FANG TaoHong,ZHANG Min,MA ChunHua,ZHENG XiaoChen,TAN WenJing,TIAN Ran,YAN Qiong,ZHOU XinLi,LI Xin,YANG SuiZhuang,HUANG KeBing,WANG JianFeng,HAN DeJun,WANG XiaoJie,KANG ZhenSheng. Application of Yr52 Gene in Wheat Improvement for Stripe Rust Resistance [J]. Scientia Agricultura Sinica, 2022, 55(11): 2077-2091.
[4] ZHANG MingJing,HAN Xiao,HU Xue,ZANG Qian,XU Ke,JIANG Min,ZHUANG HengYang,HUANG LiFen. Effects of Elevated Temperature on Rice Yield and Assimilate Translocation Under Different Planting Patterns [J]. Scientia Agricultura Sinica, 2021, 54(7): 1537-1552.
[5] XI Ling, WANG YuQi, YANG Xiu, ZHU Wei, CHEN GuoYue, WANG Yi, QIN Peng, ZHOU YongHong, KANG HouYang. Evaluation of Resistance to Stripe Rust and Molecular Detection of Resistance Gene(s) in 243 Common Wheat Landraces from the Yunnan Province [J]. Scientia Agricultura Sinica, 2021, 54(4): 684-695.
[6] ZHANG Yong,YAN Jun,XIAO YongGui,HAO YuanFeng,ZHANG Yan,XU KaiJie,CAO ShuangHe,TIAN YuBing,LI SiMin,YAN JunLiang,ZHANG ZhaoXing,CHEN XinMin,WANG DeSen,XIA XianChun,HE ZhongHu. Characterization of Wheat Cultivar Zhongmai 895 with High Yield Potential, Broad Adaptability, and Good Quality [J]. Scientia Agricultura Sinica, 2021, 54(15): 3158-3167.
[7] LIU HaiYing,FENG BiDe,RU ZhenGang,CHEN XiangDong,HUANG PeiXin,XING ChenTao,PAN YinYin,ZHEN JunQi. Relationship Between Phytohormones and Male Sterility of BNS and BNS366 in Wheat [J]. Scientia Agricultura Sinica, 2021, 54(1): 1-18.
[8] TianYu ZHOU,JiangLing LI,Lan YANG,RenWu RUAN,YuHeng YANG,ZhongAn LI. The Resistance Prediction of Wheat Hybrids Based on the Sensibility of Their Parents to Stripe Rust [J]. Scientia Agricultura Sinica, 2020, 53(9): 1806-1819.
[9] Xiao ZHANG,Man LI,DaTong LIU,Wei JIANG,Yong ZHANG,DeRong GAO. Analysis of Quality Traits and Breeding Inspiration in Yangmai Series Wheat Varieties [J]. Scientia Agricultura Sinica, 2020, 53(7): 1309-1321.
[10] MoRan XU,RuiMing LIN,FengTao WANG,Jing FENG,ShiChang XU. Evaluation of Resistance to Stripe Rust and Genetic Diversity and Detection of Resistance Genes in 103 Wheat Cultivars (Lines) [J]. Scientia Agricultura Sinica, 2020, 53(4): 748-760.
[11] HENG YanFang,LI Jian,WANG Zheng,CHEN Zhuo,HE Hang,DENG XingWang,MA LiGeng. Cloning, Expression and Functional Analysis of a Male Fertility Gene ThMs1 in Bread Wheat [J]. Scientia Agricultura Sinica, 2020, 53(23): 4727-4737.
[12] GUAN FangNian,LONG Li,YAO FangJie,WANG YuQi,JIANG QianTao,KANG HouYang,JIANG YunFeng,LI Wei,DENG Mei,LI Hao,CHEN GuoYue. Evaluation of Resistance to Stripe Rust and Molecular Detection of Important Known Yr Gene(s) of 152 Chinese Wheat Landraces from the Huang-huai-hai [J]. Scientia Agricultura Sinica, 2020, 53(18): 3629-3637.
[13] HUANG MiaoMiao,CHEN WanQuan,CAO ShiQin,SUN ZhenYu,JIA QiuZhen,GAO Li,LIU Bo,LIU TaiGuo. Surveillance and Genetic Diversity Analysis of Puccinia striiformis f. sp. tritici in Gansu and Qinghai Provinces [J]. Scientia Agricultura Sinica, 2020, 53(18): 3693-3706.
[14] TONG Jin,SUN Min,REN AiXia,LIN Wen,YU ShaoBo,WANG Qiang,FENG Yu,REN Jie,GAO ZhiQiang. Relationship Between Plant Dry Matter Accumulation, Translocation, Soil Water Consumption and Yield of High-Yielding Wheat Cultivars [J]. Scientia Agricultura Sinica, 2020, 53(17): 3467-3478.
[15] YunPeng HOU,LiLi KONG,HongGuang CAI,HuiTao LIU,YuShan GAO,YongJun WANG,LiChun WANG. The Accumulation and Distribution Characteristics on Dry Matter and Nutrients of High-Yielding Maize Under Drip Irrigation and Fertilization Conditions in Semi-Arid Region of Northeastern China [J]. Scientia Agricultura Sinica, 2019, 52(20): 3559-3572.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd