152份黄淮海麦区小麦农家品种抗条锈性评价及重要条锈病抗性基因的分子检测

doi:10.3864/j.issn.0578-1752.2020.18.001

Abstract

Abstract:

【Objective】Stripe rust of wheat (Triticum aestivum L.), caused by Puccinia striiformis f. sp. tritici, has been considered one of the most damaging diseases for wheat production in Huang-huai-hai. Identifying new resistance sources and understanding the distribution of wheat stripe rust-resistance genes in wheat landraces in this region may provide valuable resistance germplasms and molecular basis for predictive resistance rust-breeding program. 【Method】A total of 152 wheat landraces derived from Huang-huai-hai were evaluated at seedling stage in greenhouse with two Chinese predominant stripe rust races CYR32 and CYR34, and at adult-plant stage under field conditions with mixed races of CYR32, CYR33, CYR34, Su11-4 and Su11-5 in Chongzhou and Mianyang, Sichuan, during 2016-2017 and 2018-2019, respectively. The panel of wheat landraces was screened with the closely linked molecular markers of stripe rust resistance genes Yr9, Yr10, Yr18, Yr24/26, Yr30, Yr36, Yr39, Yr41, Yr48, Yr65, Yr67, Yr80, and Yr81. 【Result】Among the 152 accessions, two accessions were resistant to CYR32 and seven to CYR34, and two accessions were resistant to both CYR32 and CYR34 at seedling stage. Thirty-five (23.03%) were stably resistant to at adult-plant stage. Base on the seedling and adult-plant stage reactions, seven (4.61%) shown resistance at the all stage. Molecular detection indicated that 131 accessions carried Yr18, two carried Yr41, thirteen carried Yr48, fifty-seven carried Yr81, including 2 carried both Yr18 and Yr41, eight carried both Yr18 and Yr48, forty-nine carried both Yr18 and Yr81, seven carried both Yr81 and Yr48. All of the tested materials did not contain Yr9, Yr10, Yr24/26, Yr30, Yr36, Yr39, Yr65, Yr67 and Yr80. Simultaneously, no Yr genes were detected in the other five resistant wheat landraces and it indicated that these wheat landraces may carry other known or unknown stripe rust resistance genes. 【Conclusion】Most of wheat landraces from the Huang-huai-hai shown resistance for the current prevalence races of stripe rust at adult-plant stage and the accessions were mainly carried Yr18 and Yr81 which derived from Chinese landraces. These resistant wheat landraces may contain other known or unknown genes/their combinations and could be used as valuable resistance resources for stripe rust in wheat breeding program.

Key words: Chinese wheat landrace, stripe rust, resistance evaluation, resistance gene, molecular detection, Huang-huai-hai

GUAN FangNian,LONG Li,YAO FangJie,WANG YuQi,JIANG QianTao,KANG HouYang,JIANG YunFeng,LI Wei,DENG Mei,LI Hao,CHEN GuoYue. Evaluation of Resistance to Stripe Rust and Molecular Detection of Important Known Yr Gene(s) of 152 Chinese Wheat Landraces from the Huang-huai-hai[J].Scientia Agricultura Sinica, 2020, 53(18): 3629-3637.

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References 49

[1]	RAPILLY F. Yellow rust epidemiology. Annual Reviews of Phytopathology, 1979,17:59-73. doi: 10.1146/annurev.py.17.090179.000423
[2]	JOHNSON R. The cereal rusts. Volume II. Diseases, distribution, epidemiology and control. Physiological and Molecular Plant Pathology, 1987,30(1):151-153. doi: 10.1016/0885-5765(87)90090-7
[3]	李振岐, 曾士迈. 中国小麦锈病, 北京: 中国农业出版社, 2002: 370-373.
	LI Z Q, ZENG S M. Wheat Rust in China. Beijing: China Agriculture Press, 2002: 370-373. (in Chinese)
[4]	ZENG S M, LUO Y. Long-distance spread and interregional epidemics of wheat stripe rust in China. Plant Disease, 2006,90(8):980-988. doi: 10.1094/PD-90-0980 pmid: 30781287
[5]	WAN A M, CHEN X M, HE Z H. Wheat stripe rust in China. Australian Journal of Agricultural Research, 2007,58:605-619. doi: 10.1071/AR06142
[6]	XIA X C, LI Z F, LI G Q, SINGH R P. Stripe rust resistance in Chinese bread wheat cultivars and lines//Buck H T. Wheat Production in Stressed Environments. Dordrecht: Springer Press, 2007: 77-82.
[7]	WELLINGS C R. Global status of stripe rust: A review of historical and current threats. Euphytica, 2011,179:129-141. doi: 10.1007/s10681-011-0360-y
[8]	颜济. 五十年四川小麦育种研究的回顾与前瞻. 四川农业大学学报, 1999,17(1):108-113.
	YAN J. History and prospect of study on wheat breeding of fifty years in Sichuan. Journal of Sichuan Agricultural University, 1999,17(1):108-113. (in Chinese)
[9]	余遥. 四川小麦. 成都: 四川科学技术出版社, 1998: 397-399.
	YU Y. Sichuan Wheat. Chengdu: Sichuan Science and Technology Press, 1998: 397-399. (in Chinese)
[10]	韩德俊, 王琪琳, 张立, 魏国荣, 曾庆东, 赵杰, 王晓杰, 黄丽丽, 康振生. “西北-华北-长江中下游”条锈病流行区系当前小麦品种(系)抗条锈病性评价. 中国农业科学, 2010,43(14):2889-2896.
	HAN D J, WANG Q L, ZHANG L, WEI G R, ZENG Q D, ZHAO J, WANG X J, HUANG L L, KANG Z S. Evaluation of resistance current wheat cultivars to stripe rust in Northwest China, North China and Middle and Lower Reaches of Changjiang River epidemic area. Scientia Agricultura Sinica, 2010,43(14):2889-2896. (in Chinese)
[11]	ZENG Q D, HAN D J, WANG Q L, YUAN F P, WU J H, ZHANG L, WANG X J, HUANG L L, CHEN X M, KANG Z S. Stripe rust resistance and genes in Chinese wheat cultivars and breeding lines. Euphytica, 2014,196(2):271-284. doi: 10.1007/s10681-013-1030-z
[12]	刘太国, 王保通, 贾秋珍, 章振羽, 李强, 曹世勤, 彭云良, 金杜林, 李明菊, 刘博, 高利, 胡小平, 陈万权. 2010-2011年度我国小麦条锈菌生理专化研究. 麦类作物学报, 2012,3:574-578. doi: 10.7606/j.issn.1009-1041.2012.03.035
	LIU T G, WANG B T, JIA Q Z, ZHANG Z Y, LI Q, CAO S Q, PENG Y L, JIN S L, LI M J, LIU B, GAO L, HU X P, CHEN W Q. Physiologic specialization of Puccinia striiformis f.sp. tritici in China during 2010-2011. Journal of Triticeae Crops, 2012,32(3):574-578. (in Chinese) doi: 10.7606/j.issn.1009-1041.2012.03.035
[13]	黄瑾, 贾秋珍, 杜金林, 曹世勤, 张勃, 孙振宇, 骆惠生, 王晓明. 2010-2012年甘肃省小麦条锈病菌生理小种变化动态监测. 植物保护, 2014,3:101-105.
	HUANG J, JIA Q Z, DU J L, CAO S Q, ZHANG B, SUN Z Y, LUO H S, WANG X M. Population changes of Puccinia striiformis f. sp. tritici in Gansu Province during 2010-2012. Plant Protection, 2014,40(3):101-105. (in Chinese)
[14]	PAYNE P I, NIGHTINGLE M A, KRATTIGER A F, HOLT L M. The relationship between HMW glutenin subunit composition and the breadmaking quality of British- grown wheat varieties. Journal of the Science of Food and Agriculture, 1987,40(1):51-65. doi: 10.1002/(ISSN)1097-0010
[15]	沈裕琥, 王海庆, 杨天育, 张怀刚, 黄相国. 甘、青两省春小麦遗传多样性演变. 西北植物学报, 2002,22(4):731-740.
	SHEN Y H, WANG H Q, YANG T Y, ZHANG H G, HUANG X G. Evolvement of genetic diversity of spring wheat varieties in Gansu and Qinghai Provinces. Acta Botanica Boreali-Occidentalia Sinica, 2002,22(4):731-740. (in Chinese)
[16]	路端谊, 袁文焕, 李剑雁, 李登科, 于孝如. 小麦品种资源抗条锈病的研究. 中国农业科学, 1980,13(1):15-22.
	LU D Y, YUAN W H, LI J Y, LI D K, YU X R. Studies on stripe rust resistance of the cultivar resources of wheat. Scientia Agricultura Sinica, 1980,13(1):15-22. (in Chinese)
[17]	黄亮, 刘太国, 刘博, 高利, 罗培高, 陈万权. 黄淮麦区34个小麦主栽品种(系)抗条锈病基因推导. 植物保护, 2019,45(1):159-163.
	HUANG L, LIU T G, LIU B, GAO L, LUO P G, CHEN W Q. Postulation of yellow rust resistance genes in 34 wheat cultivars from Huanghuai Wheat Growing Region. Journal of Plant Protection, 2019,45(1):159-163. (in Chinese)
[18]	孙建鲁, 王吐虹, 冯晶, 蔺瑞明, 王凤涛, 姚强, 郭青云, 徐世昌. 100个小麦品种资源抗条锈性鉴定及重要抗条锈病基因的SSR检测. 植物保护学报, 2017,43(2):64-72.
	SUN J L, WANG T H, FENG J, LIN R M, WANG F T, YAO Q, GUO Q Y, XU S H. Identification of resistance to wheat stripe rust and detection of known resistance genes in 100 wheat cultivars with SSR markers. Journal of Plant Protection, 2017,43(2):64-72. (in Chinese)
[19]	黄苗苗, 孙振宇, 曹世勤, 贾秋珍, 刘太国, 陈万权. 223份小麦农家品种田间抗条锈病性评价及抗病基因分子检测. 植物保护学报, 2018,45(1):90-100.
	HUANG M M, SUN Z Y, CAO S Q, JIA Q Z, LIU T G, CHEN W Q. Evaluation of the resistance of 223 wheat landraces in Gansu Province to stripe rust and molecular detection. Journal of Plant Protection, 2018,45(1):90-100. (in Chinese)
[20]	王吐虹, 郭青云, 蔺瑞明, 姚强, 冯晶, 王凤涛, 陈万权, 徐世昌. 中国40个小麦农家品种和甘肃南部40个生产品种抗条锈病基因推导. 中国农业科学, 2015,48(19):3834-3847. doi: 10.3864/j.issn.0578-1752.2015.19.006
	WANG T H, GUO Q Y, LIN R M, YAO Q, FENG J, WANG F T, CHEN W Q, XU S C. 2015. Postulation of stripe rust resistance genes in Chinese 40 wheat landraces and 40 commercial cultivars in the southern region of Gansu Province. Scientia Agricultura Sinica, 2015,48(19):3834-3847. (in Chinese) doi: 10.3864/j.issn.0578-1752.2015.19.006
[21]	李峰奇, 韩德俊, 魏国荣, 曾庆东, 黄丽丽, 康振生. 黄淮麦区126个小麦品种(系)抗条锈病基因的分子检测. 中国农业科学, 2008,41(10):3060-3069.
	LI F Q, HAN D J, WEI G R, ZENG Q D, HUANG L L, KANG Z S. Molecular detection of stripe rust resistant genes in 126 winter wheat varieties from the Huanghuai Wheat Region. Scientia Agricultura Sinica, 2008,41(10):3060-3069. (in Chinese)
[22]	ROELFS A P. Race Specificity and Methods of Study. New York: Academic Press, 1984: 131-164.
[23]	MCINTOSH R A, WELLINGS C R, PARK R F. Wheat Rusts: An Atlas of Resistance Genes Berlin: Springer Netherlands Press, 1995: 9-12.
[24]	秦久刚, 熊政平. 小麦条锈病人工接种鉴定. 湖北农业科学, 1983,8(9):17-18.
	QIN J G, XIONG Z P. Identification of wheat stripe rust by artificial inoculation. Hubei Agricultural Sciences, 1983,8(9):17-18. (in Chinese)
[25]	陈万权, 刘太国, 陈巨莲, 徐世昌. 小麦抗病虫性评价技术规范. 第1部分: 小麦抗条锈病评价技术规范: NT/T1443.1-2007[S]. 2007-09-14[2017-03-03].
	CHEN W Q, LIU T G, CHEN J L, XU S C. Rules for resistance evaluation of wheat to diseases and insect pests. Part 1: Rule for resistance evaluation of wheat to yellow rust (Puccinia striiformis West. f. sp. tritici Eriks. et Henn.): NT/T1443.1-2007[S]. 2007-09-14[2017-03-03]. (in Chinese)
[26]	HILL-AMBROZ K L, BROWN-GUEDIRA G L, FELLERS J P. Modified rapid DNA extraction protocol for high throughput microsatellite analysis in wheat. Crop Science, 2002,42(6):2088-2091. doi: 10.2135/cropsci2002.2088
[27]	FRANCIS H A, LEITCH A R, KOEBNER R M. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theoretical and Applied Genetics, 1995,90(5):636-642. doi: 10.1007/BF00222127 pmid: 24174021
[28]	YUAN C L, WU J Z, YAN B Q, HAO Q Q, ZHANG C Z, LYU B, NI F, CAPLAN A, WU J J, FU D L, ZHANG C. Remapping of the stripe rust resistance gene Yr10 in common wheat. Theoretical and Applied Genetics, 2018,131(6):1253-1262. doi: 10.1007/s00122-018-3075-9 pmid: 29476226
[29]	LAGUDAH E S, KRATTINGER S G, HERRERA-FOESSEL S, SINGH R P, HUERTA-ESPINO J, SPIELMEYER W. Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theoretical and Applied Genetics, 2009,119(5):889-898. doi: 10.1007/s00122-009-1097-z
[30]	WANG C M, ZHANG Y P, HAN D J, KANG Z S, LI G P, CAO A Z, CHEN P D. SSR and STS markers for wheat stripe rust resistance gene Yr26. Euphytica, 2008,159(3):359-366. doi: 10.1007/s10681-007-9524-1
[31]	阳霞, 刘路平, 孙道杰, 张玲丽. 小麦抗病基因Lr46/Yr29/Pm39, Sr2/Yr30和Lr68的遗传特性及其与主要农艺性状的关联分析. 西北植物学报, 2014,34(3):454-462.
	YANG X, LIU L P, SUN D J, ZHANG L L. Genetic characteristics of wheat resistance gene Lr46/h29/Pm39, Sr2/Yr30 and Lr68 and association analysis of main agronomic traits. Acta Botanica Boreali- Occidentalia Sinica, 2014,34(3):454-462. (in Chinese)
[32]	SEGOVIA V, HUBBARD A, CRZAE M, BOWDEN S, WALLINGTON E, BRYANT R, GREENLAND A, BAYLES R, UAUY C. Yr36 confers partial resistance at temperatures below 18°C to U.K. isolates of Puccinia striiformis. Phytopathology, 2014,104(8):871-878. doi: 10.1094/PHYTO-10-13-0295-R
[33]	LIN F, CHEN X M. Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race-specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theoretical and Applied Genetics, 2007,114:1277-1287. doi: 10.1007/s00122-007-0518-0
[34]	LUO P G, HU X Y, REN Z L, ZHANG H Y, YANG Z J. Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS. Genome, 2008,51(11):922-927. doi: 10.1139/G08-079 pmid: 18956025
[35]	LOWE I, JANKULOSKI L, CHAO S M, CHEN X M, SEE D, DUBCOVSKY J. Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theoretical and Applied Genetics, 2011,123(1):143-157. doi: 10.1007/s00122-011-1573-0
[36]	CHEN P, XU L S, WANG M N, SEE D R, CHEN X M. Molecular mapping of genes Yr64 and Yr65 for stripe rust resistance in hexaploid derivatives of durum wheat accessions PI 331260 and PI 480016. Theoretical and Applied Genetics, 2014,127:2267-2277. doi: 10.1007/s00122-014-2378-8
[37]	XU H G, ZHANG J, ZHANG P, QIE Y M, NIU Y C, LI H J, MA P T, XU Y F, AN D A. Development and validation of molecular markers closely linked to the wheat stripe rust resistance gene YrC591 for marker-assisted selection. Euphytica, 2014,198(3):317-323. doi: 10.1007/s10681-014-1108-2
[38]	NSABIYERA V, BARIANAN H S, QRESHI M, WONG D, HAYDEN M J, BANSAL U K. Characterization and mapping of adult plant stripe rust resistance in wheat accession Aus27284. Theoretical and Applied Genetics, 2018,131:1459-1467. doi: 10.1007/s00122-018-3090-x pmid: 29560515
[39]	GESSESE M, BARIANA H, WONG D, HAYDEN M, BANSAL U. Molecular mapping of stripe rust resistance gene Yr81 in a common wheat landrace Aus27430. Plant Disease, 2019,103(6):1166-1171. doi: 10.1094/PDIS-06-18-1055-RE pmid: 30998448
[40]	LIU T G, PENG Y L, CHEN W Q, ZHANG Z Y. First detection of virulence in Puccinia striiformis f. sp. tritici in China to resistance genes Yr24 (= Yr26) present in wheat cultivar Chuanmai 42. Plant Disease, 2010,94(9):1163. doi: 10.1094/PDIS-94-9-1163A pmid: 30743706
[41]	刘太国, 章振羽, 刘博, 高利, 彭云良, 陈万权. 小麦抗条锈病基因Yr26 毒性小种的发现及其对我国小麦主栽品种苗期性分析. 植物病理学报, 2015,45(1):41-47.
	LIU T G, ZHANG Z Y, LIU B, GAO L, PENG Y L, CHEN W Q. Detection of virulence to Yr26 and pathogenicity to Chinese commercial winter wheat cultivars at seedling stage. Acta Phytopathologiga Sinica, 2015,45(1):41-47. (in Chinese)
[42]	韩德俊, 康振生. 中国小麦品种抗条锈病现状及存在问题与对策. 植物保护, 2018,44(5):1-12.
	HAN D J, KANG Z S. Current status and future strategy in breeding wheat for resistance to stripe rust in China. Plant Protection, 2018,44(5):1-12. (in Chinese)
[43]	SINGH R P. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology, 1992,82:835-838. doi: 10.1094/Phyto-82-835
[44]	杨文雄, 杨芳萍, 梁丹, 何中虎, 尚勋武, 夏先春. 中国小麦育成品种和农家种中慢锈基因Lr34/Yr18的分子检测. 作物学报, 2008,34(7):1109-1113.
	YANG W X, YANG F P, LIANG D, HE Z H, SHANG X W, XIA X C. Molecular characterization of slow-rusting genes Lr34/Yr18 in Chinese wheat cultivars. Acta Agronomica Sinica, 2008,34(7):1109-1113. (in Chinese)
[45]	白小军, 王宪国, 陈东升. 宁夏小麦品种慢锈基因Lr34/Yr18的分子检测. 麦类作物学报, 2014,34(11):1480-1484.
	BAI X J, WANG X G, CHEN D S. Molecular detection of the slow-rusting resistance gene Lr34/Yr18 in Ningxia wheat cultivars. Journal of Triticeae Crops, 2014,34(11):1480-1484. (in Chinese)
[46]	SINGH R P. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology, 1992,82:835-838.
[47]	SINGH R P, HUERTA-ESPINO J, RAJARAM S. Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica, 2000,35:133-139.
[48]	FENG J Y, WANG M A, DEVEN R S, CHAO S M, ZHENG Y L, CHEN X M. Characterization of novel gene Yr79 and four additional quantitative trait loci for all-stage and high-temperature adult-plant resistance to stripe rust in spring wheat PI 182103. Phytopathology, 2018,108(6):737-747. doi: 10.1094/PHYTO-11-17-0375-R pmid: 29303685
[49]	PAKEERATHAN K, BARIANA H, QURESHI N, WONG D, HAYDEN M, BANSAL U. Identification of a new source of stripe rust resistance Yr82 in wheat. Theoretical and Applied Genetics, 2019,132(11):3169-3176. doi: 10.1007/s00122-019-03416-y pmid: 31463519

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Evaluation of Resistance to Stripe Rust and Molecular Detection of Important Known Yr Gene(s) of 152 Chinese Wheat Landraces from the Huang-huai-hai

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[14]	HUANG MiaoMiao,CHEN WanQuan,CAO ShiQin,SUN ZhenYu,JIA QiuZhen,GAO Li,LIU Bo,LIU TaiGuo. Surveillance and Genetic Diversity Analysis of Puccinia striiformis f. sp. tritici in Gansu and Qinghai Provinces [J]. Scientia Agricultura Sinica, 2020, 53(18): 3693-3706.
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