Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (3): 413-425.doi: 10.3864/j.issn.0578-1752.2017.03.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Stripe Rust Resistance and Genes in Chongqing Wheat Cultivars and Lines

LI Bei1,2, XU Qi1,2, YANG YuHeng1,3, WANG QiLin1,4, ZENG QingDong1,4, WU JianHui1,4, MU JingMei1,2, HUANG LiLi1,4, KANG ZhenSheng1,4, HAN DeJun1,2   

  1. 1State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi; 2College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi; 3College of Plant Protection, Southwest University, Chongqing 400716; 4College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi
  • Received:2016-08-01 Online:2017-02-01 Published:2017-02-01

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Abstract: 【Objective】China has one of the largest stripe rust epidemic areas in the world. Chongqing, as an important overwintering region, plays a key role in wheat stripe rust epidemic. Understanding of resistance levels of the wheat cultivars (lines) and the application of Yr genes in this region may provide valuable recommendations for managing the disease.【Method】A total of 18 varieties and 89 advanced lines of wheat were tested at seedlings stage with four Chinese predominant stripe rust races CYR32, CYR33, V26/G22-9 and V26/CM42 of Puccinia striiformis f. sp. tritici. In field tests, wheat entries were evaluated for stripe rust resistance in Yangling, Shaanxi, artificially inoculated with mixture of CYR32 and CYR33. In Tianshui, Gansu Province, an over-summering region, the entries were evaluated under natural infection in 2015 and 2016, based on the seedling and field reactions, the resistance of the germplasms was classified and assessed. Based on the reactions of Yr single-gene lines, molecular markers for Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26were used to detect the corresponding genes.【Result】Among the 107 entries, 57 (53.27%) were resistant to both CYR32 and CYR33, 11(10.28% ) were resistant to CYR32, CYR33 and V26 / CM42, 9 (8.41%) were resistant to CYR32, CYR33 and V26/G22-9. 8 (7.48%) lines were resistance both at seedling and adult stages, 9 (8.41%) were resistant at adult plant stage, 90 (84.11%) were susceptible. Among the tested materials, 21 germplasms may contain Yr9 gene, 39 lines(varieties) may contain Yr26 gene, 17 materials may contain Yr17 gene, and 3 may contain Yr18 gene. Other materials controlled by unknown resistance genes. And Yr5, Yr10 and Yr15 were absent in the test lines. The 8 all-stage resistant materials were not detected the presence of the Yr5, Yr9, Yr10, Yr15, Yr17, Yr18 and Yr26 genes, and they may contain other resistance genes.【Conclusion】The level of resistance of wheat varieties (lines) in the region of Chongqing to tested races is extremely low, and Yr26 materials were used with high frequency especially as the rise of the V26/G22-9 and V26/CM42Yr26-virulent races. It was recommended that in order to improve the diversity of resistance gene, use of resistant varieties is important.

Key words: stripe rust, resistance genes, maker-assisted detection, Chongqing wheat region

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