谷瘟病菌生理小种鉴别及谷子标准品种体系的构建

doi:10.3864/j.issn.0578-1752.2016.17.006

Abstract

Abstract: 【Objective】Foxtail millet blast (Pyricularia setariae Nisikado) is an important disease, which could cause reduced production and quality of foxtail millet, and the identification of different millet blast physiological races are very important for evaluating the resistant resources, breeding of resistant varieties and reasonable allocation of different resistant varieties for cultivation. The goal of this study is to determine standard varieties for the identification of physiological races of millet blast fungus, which would establish a basis for the evaluation of resistance of foxtail millet to different blast races. 【Method】 Sixty foxtail millet varieties collected from different geographical regions in China were inoculated using ten foxtail millet blast isolates sampled from different eco-regions. Statistical results of the sensitive reaction of 60 foxtail millet varieties to 10 blast isolates were recorded into excel format according to 0-9 grade standards of millet blast. The different resistant results were recorded as high resistance, resistance, middle resistance, sensitivity and high sensitivity. The high resistance, resistance, middle resistance were denoted with 0, and the sensitivity and high sensitivity were denoted with 1. The results were changed to 0/1 to construct the phylogenetic tree by NTSYS. Based on the resistant and infected results of sixty varieties to ten blast isolates, the standard identification system of foxtail millet blast were constructed. 【Result】Clustering analysis based on the pathogenicity of ten blast isolates and resistance of sixty varieties was conducted, respectively. The foxtail millet blast isolates were grouped into three clusters by similarity coefficient 0.70 as boundary, and foxtail millet varieties were divided into five categories by similarity coefficient 0.65 as boundary. Seven varieties, including Danpinian, Jinpingguzi, Jingu5, Damaomaogu, Longzhuagu, Jiajinmiao and Niutougu, were applied as standard identification varieties. The resistant varieties, Huangbangtou, Qitouhuang, Danpinian, Baigu, Weizinagu, and Zhenggu4 with broad spectrum resistance were screened, which could be used as the resources of resistant gene in foxtail millet blast disease breeding. 【Conclusion】This study screened a set of standard varieties for foxtail millet blast fungus identification, and identified several genetic stocks with broad-spectrum resistance to foxtail millet breeding.

Key words: foxtail millet, Pyricularia setariae, core germplasm, physiological race, disease resistance

LI Zhi-jiang, JIA Guan-qing, LI Xiang-yu, LI Yi-chu, MA Jin-feng, ZHI Hui, TANG Sha, ZHANG Shuo, CHAI Yang, LI Yan-dong, DIAO Xian-min. Determination of Standard Varieties for Identifying Physiological Races of Foxtail Millet Blast Fungus[J].Scientia Agricultura Sinica, 2016, 49(17): 3308-3318.

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