Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (15): 3000-3008.doi: 10.3864/j.issn.0578-1752.2018.15.015

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Follicular Development Related Genes Screening and Differential Expressed Analysis by Transcriptome Sequencing in Bovine Ovary

LI PengFei1, MENG JinZhu2, JING JiongJie3, BI XiLin3, WANG Kai3, ZHU ZhiWei1, LÜ LiHua3   

  1. 1College of Life Science, Shanxi Agricultural University, Taigu 030801, Shanxi; 2Wujiang College, Tongren University, Tongren 554300, Guizhou; 3College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, Shanxi
  • Received:2017-03-28 Online:2018-08-01 Published:2018-08-01

RichHTML

8

PDF

264

Abstract: 【Objective】 The study focused on screening some genes involved in follicular development through high- throughput sequencing in bovine follicles at different physiological states. 【Method】Cows were selected for estrus synchronization, and the largest follicle and second largest follicle at predeviation during the first follicle wave were gathered by B-type ultrasonography. Thereafter, the RNA libraries were constructed and RNA sequencing was performed by Illumina platform. Compared with the database, parameters were set to screen high-expressed genes and differentially expressed genes, and then GO analysis was conducted. Further screened regulatory genes directly related to follicular development by Genecards, and qRT-PCR was performed to validate expression of screened genes associated with follicular development. 【Result】 Results showed that 263 differentially expressed genes were obtained from the two transcripts, which could be assigned into 90 groups under three categories by GO clustering analysis (biological processes, 64.4%; cell component, 17.8%; molecular function, 17.8%). Some important functional enrichment pathways were obtained, such as regulating signal transduction and cytokine response biological pathways; 10 high-expressed up-regulated and down-regulated genes were selected by gene expression analysis, CYP17A1 involved in estrogen synthesis and fetal gender development, LOC785462 participated in steroid hormone synthesis, DACH1 regulated cell apoptosis in process of cell development, and MAP3K3 regulated ERK and MEK1/2 signaling pathways. Six genes were found closely associate with follicular development by Genecards: up-regulated genes included PRSS35, PTGRF, ARID4B and GPR116, down-regulated genes included APOA1 and CPXM1. qRT-PCR results showed that expression of PRSS35 and ARID4B were significantly higher in DF than in SF (P＜0.05), and CPXM1 expression was significantly higher in SF than in DF (P＜0.01). There was no significant difference in the expression of PTGRF, GPR116 and APOA1 between DF and SF (P＞0.05), however, the expression variation trend was consistent with high-throughput sequencing results. 【Conclusion】The transcriptome sequencing results were accurate, PRSS35 and ARID4B played positive roles and CPXM1 played a negative role in regulating follicular development, genes related to bovine follicular development and important regulated pathways, which were of great significance to further study the regulation mechanism of follicular development.

Key words: bovine, follicle, transcript, PDF1, PDF2, development

[1]   BEG M A, BERGFELT D R, KOT K, GINTHER O J. Follicle selection in cattle: dynamics of follicular fluid factors during development of follicle dominance. Biology of Reproduction, 2002, 66(1): 120-126.
[2]    TERENINA E, FABRE S, BONNET A, MONNIAUX D, ROBERT- GRANIÉ C, SANCRISTOBAL M, SARRY J, VIGNOLES F, GONDRET F, MONGET P, TOSSER-KLOPP G. Differentially expressed genes and gene networks involved in pig ovarian follicular atresia. Physiological Genomics, 2017, 49(2):67-80.
[3]    TICIANELLI J S, EMANUELLI I P, SATRAPA R A, CASTILHO A C, LOUREIRO B, SUDANO M J, FONTES P K, PINTO R F, RAZZA E M, SURJUS R S, SARTORI R, ASSUMPCAO M E, VISINTIN J A, BARROS C M, PAULA-LOPES F F. Gene expression profile in heat-shocked Holstein and Nelore oocytes and cumulus cells. Reproduction Fertility and Development, 2016, doi: 10.1071/ RD16154.
[4]    LI P, MENG J, LIU W, SMITH G W, YAO J, LYU L. Transcriptome analysis of bovine ovarian follicles at predeviation and onset of deviation stages of a follicular wave. International Journal of Genomics, 2016, doi:10.1155/2016/3472748.
[5]    SAVIO J D, KEENAN L, BOLAND M P, ROCHE J F. Pattern of growth of dominant follicles during the oestrous cycle of heifers. Journal of Reproduction and Fertility, 1988, 83(2):663-671.
[6]    SIROIS J, FORTUNE J E. Ovarian follicular dynamics during the estrous cycle in heifers monitored by real-time ultrasonography. Biology of Reproduction, 1988, 39(2):308-317.
[7]    李鹏飞，毕锡麟，王锴，景炅婕，吕丽华. CART在不同发育阶段牛卵泡颗粒细胞中的表达和定位. 中国农业科学，2016, 49(12): 2389-2396.
LI P F, BI X L, WANG K, JING J J, LÜ L H. Research on the expression and localization of CART in bovine granulosa cells at different developmental stages. Scientia Agricultura Sinica, 2016, 49(12):2389-2396. (in Chinese)
[8]   李鹏飞, 孟金柱, 谢建山, 朱芷葳, 刘岩, 姜晓龙, 陈建伟, 姚晓磊, 赵妙妙, 吕丽华. 牛卵泡ODF1 与ODF2 转录组发育相关基因筛选及表达差异分析. 畜牧兽医学报，2015, 46(11):1961-1966.
LI P F, MENG J Z, XIE J S, ZHU Z W, LIU Y, JIANG X L, CHEN J W, YAO X L, ZHAO M M, LÜ L H. Screening and analyse study of genes associated with follicular development in bovine ODF1 and ODF2 transcript. Acta Veterinaria et Zootechnica Sinica, 2015, 46(11): 1961-1966. (in Chinese)
[9]    AUDIC S, CLAVERIE J M. The significance of digital gene expression profiles. Genome Research, 1997, 7(10):986-995.
[10]   李鹏飞. 牛卵泡可卡因-苯丙胺调节转录肽（CART）受体的筛选[D]. 太谷：山西农业大学，2014.
LI P F. Screening of cocaine-and amphetamine-regulated transcript peptide (CART) receptor of cattle follicle [D]. Taigu: Shanxi Agricultural University, 2014. (in Chinese)
[11]   WANG Z, GERSTEIN M, SNYDER M. RNA-Seq: a revolutionary tool for transcriptomics. Nature Reviews Genetics, 2009, 10(1):57-63.
[12]   HAAS B J, ZODY M C. Advancing RNA-Seq analysis. Nature Biotechnology, 2010, 28(5):421-423.
[13]   MARIONI J C, MASON C E, MANE S M, STEPHENS M, GILAD Y. RNA-seq: an assessment of technical reproducibility and comparison with gene expression arrays. Genome Research, 2008, 18(9): 1509-1517.
[14]   CROUCHER N J, FOOKES M C, PERKINS T T, TURNER D J, MARGUERAT S B, KEANE T, QUAIL M A, HE M, ASSEFA S, BÄHLER J, KINGSLEY R A, PARKHILL J, BENTLEY S D, DOUGAN G, THOMSON N R. A simple method for directional transcriptome sequencing using Illumina technology. Nucleic Acids Research, 2009, 37(22): e148.
[15]   SOON W W, MILLER L D, BLACK M A, DALMASSO C, CHAN X B, PANG B, ONG C W, SALTO-TELLEZ M, DESAI K V, LIU E T. Combined genomic and phenotype screening reveals secretory factor SPINK1 as an invasion and survival factor associated with patient prognosis in breast cancer. EMBO Molecular Medicine, 2011, 3(8): 451-464.
[16]   DIAO H, XIAO S, LI R, ZHAO F, YE X. Distinct spatiotemporal expression of serine proteases Prss23 and Prss35 in periimplantation mouse uterus and dispensable function of Prss35 in fertility. PLoS One, 2013, 8(2):e56757.
[17]   WAHLBERG P, NYLANDER A, AHLSKOG N, LIU K, NY T. Expression and localization of the serine proteases high-temperature requirement factor A1, serine protease 23, and serine protease 35 in the mouse ovary. Endocrinology, 2008, 149(10):5070-5077.
[18]   LI S H, LIN M H, HWU Y M, LU C H, YEH L Y, CHEN Y J, LEE R K. Correlation of cumulus gene expression of GJA1, PRSS35, PTX3, and SERPINE2 with oocyte maturation, fertilization, and embryo development. Reproductive Biology and Endocrinology, 2015, 13:93. doi: 10.1186/s12958-015-0091-3.
[19]   KIM S, ZHANG Z, UPCHURCH S, ISERN N, CHEN Y. Structure and DNA-binding sites of the SWII AT-rich interaction domain (ARID) suggest determinants for sequence-specific DNA recognition. Journal of Biological Chemistry, 2004, 279(16):16670-16676.
[20]   HERRSCHER R F, KAPLAN M H, LELSZ D L, DAS C, SCHEUERMANN R, TUCKER P W. The immunoglobulin heavy chain matrix associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family. Genes & Development, 1995, 9(24):3067-3082.
[21]   WILSKER D, PATSIALOU A, DALLAS P B, MORAN E. ARID proteins: a diverse family of DNA binding proteins implicated in the control of cell growth, differentiation, and development. Cell Growth & Differentiation, 2002, 13(3):95-106.
[22]   LAI A, MARCELLUS R C, CORBEIL H B, BRANTON P E. RBP1 induces growth arrest by repression of E2F-dependent transcription. Oneogene, 1999, 18(12):2091-2100.
[23]   CAO J, GAO T, STANBRIDGE E J, IRIE R. RBP1L1, a retinoblastoma- binding protein-related gene encoding an antigenic epitope abundantly expressed in human carcinomas and normal testis. Journal of the National Cancer Institute, 2001, 93(15):1159-1165.
[24]   ATEEQ B, TOMLINS S A, LAXMAN B, ASANGANI I A, CAO Q, CAO X, LI Y, WANG X, FENG F Y, PIENTA K J, VARAMBALLY S, CHINNAIYAN A M. Therapeutic targeting of SPINK1-positive prostate cancer. Science Translational Medicine, 2011, 3(72): 72ra17.
[25]   GHADGE G D, SLUSHER B S, BODNER A, CANTO M D, WOZNIAK K, THOMAS A G, ROJAS C, TSUKAMOTO T, MAJER P, MILLER R J, MONTI A L, ROOS R P. Glutamate carboxypeptidase II inhibition protects motor neurons from death in familial amyotrophic lateral sclerosis models. Proceedings of the National Academy of Sciences of the USA, 2003, 100(16):9554-9559.
[26]   GOMIS-RÜTH F X, COMPANYS V, QIAN Y, FRICKER L D, VENDRELL J, AVILÉS F X, COLL M. Crystal structure of avian carboxypeptidase D domain II: a prototype for the regulatory metallocarboxypeptidase subfamily. EMBO Journal, 1999, 18(21): 5817-5826.
[27]   KIM Y H, O'NEILL H M, WHITEHEAD J P. Carboxypeptidase X-1 (CPX-1) is a secreted collagen-binding glycoprotein. Biochemical and Biophysical Research Communications, 2015, 468(4):894-899.
[28]   CHANG E J, KWAK H B, KIM H, PARK J C, LEE Z H, KIM H H. Elucidation of CPX-1 involvement in RANKL-induced osteoclastogenesis by a proteomics approach. FEBS Letters, 2004, 564(1/2):166-170.
[29]   LEI Y, XIN X, MORGAN D, PINTAR J E, FRICKER L D. Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene family with highest similarity to CPX-2. DNA and Cell Biology, 1999, 18(2):175-185.
[30]   UEHIRO N, SATO F, PU F, TANAKA S, KAWASHIMA M, KAWAGUCHI K, SUGIMOTO M, SAJI S, TOI M. Circulating cell-free DNA-based epigenetic assay can detect early breast cancer. Breast Cancer Research, 2016, 18(1):129-142.
[1] LIN XinYing,WANG PengJie,YANG RuXing,ZHENG YuCheng,CHEN XiaoMin,ZHANG Lei,SHAO ShuXian,YE NaiXing. The Albino Mechanism of a New High Theanine Tea Cultivar Fuhuang 1 [J]. Scientia Agricultura Sinica, 2022, 55(9): 1831-1845.
[2] XIE LingLi,WEI DingYi,ZHANG ZiShuang,XU JinSong,ZHANG XueKun,XU BenBo. Dynamic Changes of Gibberellin Content During the Development and Its Relationship with Yield of Brassica napus L. [J]. Scientia Agricultura Sinica, 2022, 55(24): 4793-4807.
[3] YOU YuWan,ZHANG Yu,SUN JiaYi,ZHANG Wei. Genome-Wide Identification of NAC Family and Screening of Its Members Related to Prickle Development in Rosa chinensis Old Blush [J]. Scientia Agricultura Sinica, 2022, 55(24): 4895-4911.
[4] ZHANG Jie,JIANG ChangYue,WANG YueJin. Functional Analysis of the Interaction Between Transcription Factors VqWRKY6 and VqbZIP1 in Regulating the Resistance to Powdery Mildew in Chinese Wild Vitis quinquangularis [J]. Scientia Agricultura Sinica, 2022, 55(23): 4626-4639.
[5] TU YunJie,JI GaiGe,ZHANG Ming,LIU YiFan,JU XiaoJun,SHAN YanJu,ZOU JianMin,LI Hua,CHEN ZhiWu,SHU JingTing. Screening of Wnt3a SNPs and Its Association Analysis with Skin Feather Follicle Density Traits in Chicken [J]. Scientia Agricultura Sinica, 2022, 55(23): 4769-4780.
[6] YOU JiaLing,LI YouMei,SUN MengHao,XIE ZhaoSen. Analysis Reveals the Differential Expression of Genes Related to Starch Accumulation in Chloroplast of Leaf with Different Ages in Pinot Noir Grape [J]. Scientia Agricultura Sinica, 2022, 55(21): 4265-4278.
[7] SUN BaoJuan,WANG Rui,SUN GuangWen,WANG YiKui,LI Tao,GONG Chao,HENG Zhou,YOU Qian,LI ZhiLiang. Transcriptome and Metabolome Integrated Analysis of Epistatic Genetics Effects on Eggplant Peel Color [J]. Scientia Agricultura Sinica, 2022, 55(20): 3997-4010.
[8] LIU Xin,ZHANG YaHong,YUAN Miao,DANG ShiZhuo,ZHOU Juan. Transcriptome Analysis During Flower Bud Differentiation of Red Globe Grape [J]. Scientia Agricultura Sinica, 2022, 55(20): 4020-4035.
[9] PANG HaoWan,FU QianKun,YANG QingQing,ZHANG YuanYuan,FU FengLing,YU HaoQiang. Maize Transcription Factor ZmEREB93 Negatively Regulates Kernel Development [J]. Scientia Agricultura Sinica, 2022, 55(19): 3685-3696.
[10] LI LiYing,HE YingTing,ZHONG YuYi,ZHOU XiaoFeng,ZHANG Hao,YUAN XiaoLong,LI JiaQi,CHEN ZanMou. CTNNB1 Regulates the Function of Porcine Ovarian Granulosa Cells [J]. Scientia Agricultura Sinica, 2022, 55(15): 3050-3061.
[11] YANG ShengDi,MENG XiangXuan,GUO DaLong,PEI MaoSong,LIU HaiNan,WEI TongLu,YU YiHe. Co-Expression Network and Transcriptional Regulation Analysis of Sulfur Dioxide-Induced Postharvest Abscission of Kyoho Grape [J]. Scientia Agricultura Sinica, 2022, 55(11): 2214-2226.
[12] LIU RuiDa, GE ChangWei, WANG MinXuan, SHEN YanHui, LI PengZhen, CUI ZiQian, LIU RuiHua, SHEN Qian, ZHANG SiPing, LIU ShaoDong, MA HuiJuan, CHEN Jing, ZHANG GuiYin, PANG ChaoYou. Cloning and Drought Resistance Analysis of Transcription Factor GhMYB108 in Gossypium hirsutum [J]. Scientia Agricultura Sinica, 2022, 55(10): 1877-1890.
[13] ZHANG Jing,ZHANG JiYue,YUE YongQi,ZHAO Dan,FAN YiLing,MA Yan,XIONG Yan,XIONG XianRong,ZI XiangDong,LI Jian,YANG LiXue. LKB1 Regulates Steroids Synthesis Related Genes Expression in Bovine Granulosa Cells [J]. Scientia Agricultura Sinica, 2022, 55(10): 2057-2066.
[14] MA ShuanHong, WAN Jiong, LIANG RuiQing, ZHANG XueHai, QIU XiaoQian, MENG ShuJun, XU NingKun, LIN Yuan, DANG KunTai, WANG QiYue, ZHAO JiaWen, DING Dong, TANG JiHua. Candidate Gene Association Analysis of Maize Transcription Factors in Flowering Time [J]. Scientia Agricultura Sinica, 2022, 55(1): 12-25.
[15] GUO YongChun, WANG PengJie, JIN Shan, HOU Binghao, WANG ShuYan, ZHAO Feng, YE NaiXing. Identification of Co-Expression Gene Related to Tea Plant Response to Glyphosate Based on WGCNA [J]. Scientia Agricultura Sinica, 2022, 55(1): 152-166.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd