Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (9): 1617-1626.doi: 10.3864/j.issn.0578-1752.2018.09.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Identification and Gene Mapping of a Shorten Panicle and Seed Mutant sps1 in Rice (Oryza sativa L.)

XIE Jia, ZHANG XiaoBo, TAO YiRan, XIONG YuZhen, ZHOU Qian, SUN Ying, YANG ZhengLin, ZHONG BingQiang, SANG XianChun   

  1. Institute of Rice Research, Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Chongqing 400715
  • Received:2017-12-26 Online:2018-05-01 Published:2018-05-01

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Abstract: 【Objective】Identification and gene mapping of a shorten panicle and seed mutant is significant for rice functional genomics research and molecular breeding. 【Method】A shorten panicle and seed mutant (sps1) was identified from the EMS-induced library. At the maturity stage, agronomic traits such as plant height, panicle length, seed number per panicle, filled grain number per panicle, seedsetting rate and 1000-seeds weight were measured. Scanning electron microscopy was carried out to analyze the inner and outer epidermis between the wild type and sps1 and the grain morphology of wild type and sps1 was observed by paraffin section. The sps1 was crossed with Jinhui 10, and the F1 and F2 generations were used for genetic analysis. Sensitivity test of brassinolide (BR) was carried out on sheath of wild-type and sps1. The root, stem, leaf, sheath and spike were collected, and the genes associated with grain development and the genes associated with BR were analyzed in qPCR.【Result】The sps1 was dwarf in all phases of plant development, and the panicle length, the first, second and third internodes were significantly shorter than those of the wild type. In addition, there were significant decrease in the branch number, seed-setting rate and the 1000-seeds weight in the sps1 mutant. The results of scanning electron microscopy showed that when compared with the wild type, the inside and outside the central lemma of epidermal cells of sps1 was shorter and broader, and paraffin section showed that the smaller seed size of sps1 was caused by reduced cell length, increased cell width and cell number. There were significant changes in the expression of genes regulating rice grain size by controlling cell extension and division, such as the AFD1, SLG, HGW and GS3 significantly increased, the GW7 and GID1 significant reduced. Choose the single mutant strains in F2 generation of Jinhui10/sps1 accord with the 3:1 separation ratio to conduct gene localization. And SPS1 was finally mapped on chromosome 7 with a 134 kb physical distance between markers sps1-3 and sps1-2. There are 19 annotated genes in the fine mapping region. Sequencing indicated that a single nucleotide substitution from A to T occurred in Os07g0616000, and a lysine was changed into termination codon, and then leaded to protein coding early termination in sps1. The root, stem, leaf, sheath and spike of the wild type and sps1 were analyzed by qPCR, and the results showed that the target gene was expressed in each organ of the plant, and the expression was highest in the plant stem. Bioinformatics analysis showed that SPS1 was a new allele of DEP2. The sensitivity of sps1 to the exogenous BR was reduced, and the expression of D1 was significantly reduced. It is speculated that SPS1/DEP2 may regulate the development of rice grain and plants by BR signaling pathway.【Conclusion】sps1 was a shorten panicle and seed mutant. SPS1 encoded an expression protein and is a new allele of DEP2, which regulated the development of rice grain and plant type by BR signaling pathway.

Key words: rice (Oryza sativa L.), shorten panicle, small grain, gene mapping

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