碱胁迫对小麦（Triticum aestivum Linn）叶片代谢过程的影响

doi:10.3864/j.issn.0578-1752.2017.02.005

Abstract

Abstract: 【Objective】 A pot experiment was conducted to investigate the alkaline stress in ion balance and metabolic profiles of wheat, to determine the physiological adaptive mechanisms of wheat in tolerance to alkali stress. 【Method】 In a pot experiment with control and alkaline stress (NaHCO₃﹕Na₂CO₃=1﹕1), the growth and photosynthetic characters, ion and 73 key metabolites of wheat were studied. 【Result】The results showed that when alkaline stress intensity exceeded the capacity of wheat adjustment, Na⁺ accumulation in cells in a high-pH environment resulted in damage of the photosynthetic system, reduced photosynthetic pigments, inhibited the activity of photosystem II, and reduced high stomatal conductance and net photosynthetic rate. Alkaline stress caused massive influx of Na⁺, a decrease of inorganic negative charge and pH value homeostasis, thus resulting ionic unbalance and leading to a series of strain metabolic response. In addition, 73 metabolites were detected in different alkaline stress treatments according to GC-MS analysis, and these metabolites were sugars/polyols, organic acids, amino acids and others. Compared with the control sample, the response of 25 and 48 metabolites in moderate and severe alkaline stress treatments remarkably changed, respectively, in leaves of wheat seedlings. The results of one-way ANOVA analysis indicated that the changes of metabolites were more significant under high alkaline stress than that under moderate alkaline stress. The results revealed that alkaline stress caused an significant decrease in levels of 5 and 6 metabolites, which are involved in TCA cycle and glycolysis; it also caused amino acids (glutamate, alanine, γ-aminobutyric acid, aspartic acid) and sugars/ploys (fructose, sucrose, talose, myo-inositol) decreased dramatically. Meanwhile, alkaline stress induced organic acids accumulation in wheat, and it maybe a passive adaptive response to alkaline stress, and organic acids kept ionic balance and pH homeostasis. 【Conclusion】 The results suggested that alkaline stress caused systems alterations in metabolic networks including TCA cycle, glycolysis, calvin cycle, shikimic path way, metabolism of plasma membrane,GS/GOGAT cycle and GABA path way, implying alkaline stress not only had side effect on synthesis of sugars, amino acids, fats and proteins, but also inhibited the translation between C and N, thus resulted in nutrients deficiency and caused decrease of plant growth and development.

Key words: wheat (Triticum aestivum Linn), alkali stress, leaves, growth characters, photosynthetic characters, metabonomic

GUO Rui, ZHOU Ji, YANG Fan, LI Feng. Effects of Alkaline Stress on Metabonomic Responses of Wheat (Triticum aestivum Linn) Leaves[J].Scientia Agricultura Sinica, 2017, 50(2): 250-259.

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Effects of Alkaline Stress on Metabonomic Responses of Wheat (Triticum aestivum Linn) Leaves

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