基于GC-MS技术的蹄叶炎奶牛血浆代谢谱分析

doi:10.3864/j.issn.0578-1752.2016.21.020

Abstract

Abstract: 【Objective】In order to discover the difference between the cows suffered with laminitis and healthy cows, metabonomics analysis based on gas chromatograph tandem mass spectrometer technology (GC-MS) was utilized in the study.【Method】Twenty plasma samples were collected from cows with laminitis and healthy groups. Plasma metabolic profiling was obtained by GC-MS after derivatization. Multivariate statistics including principal component analysis (PCA) and supervised partial least squares-discriminant analysis (PLS-DA) were conducted to screen the changed metabolites. Bioinformatics was analyzed to identify the significantly changed metabolic pathways.【Result】A total of 242 variables were detected and 37 compounds were significantly different between healthy and laminitis-suffered cows of which 3 were up-regulated including aminooxyacetic acid, lactose and oleic acid and 34 were down-regulated in laminitis group involved amino acid and fatty acid. The main changed metabolic pathways were fatty acid biosynthesis, Gly, Ser, Thr metabolism, biosynthesis of unsaturated fatty acids, purine metabolism, methane metabolism, cyanoamino acid metabolism and phenylalanine metabolism. Results of the the experiment of validation showed that the indexes related to fatty acid metabolism and antioxidation were significantly different between healthy cows and sick cows. 【Conclusion】 Based on GC-MS, this study revealed the changed regularity of metabolism in plasma with laminitis, and the discovered different metabolites would be potential biomarkers in occurrence and development process of laminitis, which can lay a theoretical foundation for further understanding of the pathogenesis, early diagnosis and therapy of laminitis in dairy cows.

Key words: metabonomics, laminitis, gas chromatograph tandem mass spectrometer technology, dairy cow

LI Ya-juan, WANG Dong-sheng, ZHANG Shi-dong, YAN Zuo-ting, YANG Zhi-qiang, DU Yu-lan, DONG Shu-wei, HE Bao-xiang. Plasma Metabolic Profiling Analysis of Dairy Cows with Laminitis Based on GC-MS[J].Scientia Agricultura Sinica, 2016, 49(21): 4255-4264.

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Plasma Metabolic Profiling Analysis of Dairy Cows with Laminitis Based on GC-MS

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