干旱胁迫对小麦不同品种胚乳淀粉结构和理化特性的影响

doi:10.3864/j.issn.0578-1752.2017.02.006

Abstract

Abstract: 【Objective】The objective of this experiment is to study the effects of drought stress on the composition, granule size distribution, pasting properties and crystal properties of wheat endosperm of different varieties, and reveal the intrinsic relationship between the structure and physicochemical properties of wheat starch. 【Method】The wheat cultivars Jingdong8(JD8), Henong825(HN825), Jimai585(JM585) and Nongda211(ND211) were used as the test materials, and irrigated and rainfed treatments were designed in research of the effects of drought stress on wheat endosperm starch structure and physicochemical properties. 【Result】Drought stress significantly inhibited the accumulation of starch in wheat endosperm, but it had no significant effect on the Am/Ap ratio of wheat starch. The granule volume, surface area and number distribution of wheat endosperm showed a bimodal curve. The effect of drought stress on starch grain size distribution of wheat endosperm was different due to the difference of genotype and grain size, in which it had a great influence on the surface area and the number distribution of starch granule size ＜5 μm. Drought stress didn’t change the crystal type of wheat endosperm starch, but significantly increased the crystallinity of starch, and the effects of drought stress on intensity of X-ray diffraction peak were different due to different cultivars and diffraction angles. Drought stress significantly increased trough viscosity and pasting temperature of starch, prolonged the pasting time, while significantly reduced the peak viscosity and breakdown, and there was a genotypic difference in final viscosity and setback. Correlation analysis showed a that the wheat endosperm starch crystallinity showed significant negative correlation with total starch and Am content, and a significant positive correlation with Ap content. The total starch content showed a significant positive correlation with peak viscosity. The Am content showed a significant positive correlation with peak viscosity and breakdown, and a negative correlation with the pasting temperature. The correlation between the Ap content and pasting parameters was not significant, while the Am/Ap ratio of wheat starch showed a significant negative correlation with the pasting time. The volume of ＜5 μm and ＜10 μm starch granule, respectively, showed a significant and an extreme significant negative correlation with final viscosity and setback, but the starch granule with diameter ＞15 μm was positively correlated with final viscosity and setback. The correlation between granule volume distribution and crystallinity of wheat was not significant. Wheat starch peak viscosity and breakdown, respectively, showed an extremely significant and significant negative correlation with crystallinity, but there was a significant positive correlation between the pasting temperature and crystallinity. 【Conclusion】Drought stress changed the wheat endosperm starch composition, granule size distribution, crystallinity and pasting parameters. There were significant correlations between wheat endosperm starch structure and physicochemical properties, indicating that the effects of drought stress on the structure of wheat starch, indirectly affected the physicochemical properties, therefore, the cultivation conditions could be adjusted to achieve the purpose of quality wheat production.

Key words: wheat, starch, drought stress, starch structure, crystal properties, pasting properties

SONG XiaoJun, ZHANG Min, WU XuePing, ZHAO Cheng, SHI Jian, ZHANG YuChun, LIU XiWei, CAI RuiGuo. Effects of Drought Stress on Wheat Endosperm Starch Structure and Physicochemical Properties of Different Varieties[J].Scientia Agricultura Sinica, 2017, 50(2): 260-271.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2017.02.006

https://www.chinaagrisci.com/EN/Y2017/V50/I2/260

References

[1] 阎俊, 张勇, 何中虎. 小麦品种糊化特性研究. 中国农业科学, 2001, 34(1): 1-4.

Yan J, Zhang Y, He Z H. Investigation on paste property of Chinese wheat. Scientia Agricultura Sinica,2001, 34(1): 1-4. (in Chinese)

[2] Jane J L, Chen J F. Effect of amylose molecular size and amylopectin branch chain length on paste properties of starch. Cereal Chemistry, 1992, 69(1): 60-65.

[3] 蔡瑞国, 张敏, 韩婷, 谢新宇, 徐彩龙, 顾锋. 种植密度对小麦籽粒淀粉含量和晶体特性的影响. 麦类作物学报, 2013, 34(1): 78-83.

Cai R G, Zhang M, Han T, Xie X Y, Xu C L, Gu F. Effect of plant density crystal property starch content and wheat grain. Journal of Triticeae Crops, 2013, 34(1): 78-83. (in Chinese)

[4] 王晨阳, 张艳菲, 卢红芳, 赵君霞, 马耕, 马冬云, 朱云集, 郭天财, 马英, 姜玉梅. 花后渍水、高温及其复合胁迫对小麦籽粒淀粉组成与糊化特性的影响. 中国农业科学, 2015, 48(4): 813-820.

Wang C Y, Zhang Y F, Lu H F, Zhao J X, Ma G, Ma D Y, Zhu Y J, Guo T C, Ma Y, Jiang Y M. Effects of post-anthesis waterlogging, high temperature and their combination on starch compositions and pasting properties in wheat grains. Scientia Agricultura Sinica, 2015, 48(4): 813-820. (in Chinese)

[5] 李诚, 张润琪, 付凯勇, 李春艳. 花后干旱对小麦胚乳淀粉粒发育和理化特性的影响. 麦类作物学报, 2015, 35(9): 1284-1290.

Li C, Zhang R Q, Fu K Y, Li C Y. Effect of drought post anthesis on the development and physicochemical characteristics of starch granule in wheat. Journal of Triticeae Crops, 2015, 35(9): 1284-1290. (in Chinese)

[6] 曹龙奎, 李凤林. 淀粉颗粒的结构. 北京: 中国轻工业出版社, 2011.

Cao L K, Li F L. The Structure of Starch Granules. Beijing: China Light Industry Press,2011. (in Chinese)

[7] RINDLAV-WESTLING A, STADING M, GATENHOLM P. Crystallinity and morphology in films of starch, amylose and amylopectin blends. Biomacromolecules, 2002, 3(1): 84-91.

[8] 王珏, 封超年, 郭文善, 朱新开, 李春燕, 彭永欣. 花后高温胁迫对小麦籽粒淀粉积累及晶体特性的影响. 麦类作物学报, 2008, 28(2): 260-265.

Wang J, Feng C N, Guo W S, Zhu X K, Li C Y, Peng Y X. Effects of high temperature starch traits of grain after anthesis on in wheat. Journal of Triticeae Crops, 2008, 28(2): 260-265. (in Chinese)

[9] Tang H, Mitsunaga T, Kawamura Y. Molecular arrangement in blocklets and starch granule architecture. Carbohydrate Polymers, 2006, 63(4): 555-560.

[10] 宋健民, 刘爱峰, 李豪圣, 戴双, 刘建军, 赵振东, 刘广田. 小麦籽粒淀粉理化特性与面条品质关系研究. 中国农业科学, 2008, 41(1): 272-279.

Song J M, Liu A F, Li H S, Dai S, Liu J J, Zhao Z D, Liu G T. Relationship between starch physiochemical properties of wheat grain and noodle quality. Scientia Agricultura Sinica, 2008, 41(1): 272-279. (in Chinese)

[11] Hurkman W J, McCue K F, Altenbach S B, Korn A, Tanaka C K, Kothari K M, Johnson E L, Bechtel D B, Wilson J D, Anderson O D, DuPont F M. Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm. Plant Science, 2003, 164: 873-881.

[12] Kim H S, Huher K C. Channels within soft wheat starch A-and B-type granules. Journal of Cereal Science, 2008, 48: 159-172.

[13] grejas G, Faucher B, Berirand D, Guibert D, Leroy P, Branlard G. Genetic analysis of the size of endosperm starch granules in a mapped segregating wheat population. Journal of Cereal Science, 2002, 35: 103-107.

[14] Zhang T, Wang Z, Yin Y, Cai R, Yan S, Li W. Starch content and granule size distribution in grains of wheat in relation to post-anthesis water deficits. Journal of Agronomy & Crop Science, 2010, 196: 1-8.

[15] 徐云姬, 李银银, 钱希旸, 王志琴, 杨建昌. 三种禾谷类作物强、弱势粒淀粉粒形态与粒度分布的比较. 作物学报, 2016, 42(1): 70-81.

Xu Y J, Li Y Y, Qian X Y, Wang Z Q, Yang J C. Comparison of starch granule morphology and size distribution in superior and inferior grains of three cereal crops. Acta Agronomica Sinica, 2016, 42(1): 70-81. (in Chinese)

[16] 戴忠民, 尹燕枰, 郑世英, 蔡瑞国, 顾锋, 闫素辉, 李文阳, 王振林. 不同供水条件对小麦强、弱势籽粒中淀粉粒度分布的影响. 生态学报, 2009, 29(12): 6534-6543.

Dai Z M, Yin Y P, Zheng S Y, Cai R G, Gu F, Yan S H, Li W Y, Wang Z L. Effect of water regime on starch granule size distribution in superior and inferior grains of wheat. Acta Ecologica Sinica, 2009, 29(12): 6534-6543. (in Chinese)

[17] Peng D L, Cai T, Yin Y P, Yang W B, Ni Y L, Yang D Q, Wang Z L. Exogenous application of abscisic acid or gibberellin acid has different effects on starch granule size distribution in grains of wheat. Journal of Integrative Agriculture, 2013, 12: 1551-1559.

[18] Morris C F, Shackley B J, King G E, Kidwell K K. Genotypic and environmental variation for flour swelling volume in wheat. Cereal Chemistry, 1997, 74: 16-21.

[19] Peng M, Gao M, Esm A A, Hucl P, Chibbar R N. Separation and characterization of A-and B-type starch granules in wheat endosperm. Cereal Chemistry, 1999, 76(3): 375-379.

[20] MANDAL D, TUDU S, MITRA S R, DE G C. Effect of common packing materials on keeping quality of sugarcane jaggery during monsoon season. Sugar Tech, 2006, 8(2/3): 137-142.

[21] 陆国权, 唐忠厚, 黄华宏. 不同施钾水平甘薯直链淀粉含量和糊化特性的基因型差异. 浙江农业学报, 2005, 17(5): 280-283.

Lu G Q, Tang Z H, Huang H H. Genotype variation in amylose content and strach pasting properties of sweetpotato storage at two K levels. Acta Agriculturae Zhejiangensis, 2005, 17(5): 280-283. (in Chinese)

[22] 蔡瑞国, 尹燕枰, 张敏, 戴忠民, 严美玲, 付国占, 贺明荣, 王振林. 氮素水平对藁城8901和山农1391籽粒品质的调控效应. 作物学报, 2007, 33(2): 304-310.

Cai R G, Yin Y P, Zhang M, Dai Z M, Yan M L, Fu G Z, He M R, Wang Z L. Effects of nitrogen application rate on grain quality in wheat cultivars GC8901 and SN1391. Acta Agronomica Sinica, 2007, 33(2): 304-310. (in Chinese)

[23] Lu H F, Wang C y, Guo T c, Xie Y x, Feng W, Li S y. Starch composition and its granules distribution in wheat grains in relation to post-anthesis high temperature and drought stress treatment. Starch/Stärke, 2014, 66: 419-428.

[24] Paul C. The structure of starch. Nature, 1997, 389: 338-339.

[25] Tester R F, Karkalas J, Qi X. Starch structure and digestibility enzyme-substrate relationship. Worlds Poultry Science Journal, 2004, 60: 186-195.

[26] Panozzo J F, Eagles H A. Cultivars and environmental effects on quality characters in wheat: I.Starch. Australian Journal of Agricultural Research, 1998, 49: 757-766.

[27] 蔡瑞国, 尹燕杆, 赵发茂, 张敏, 张体彬, 梁太波, 顾锋, 戴忠民, 王振林. 强筋小麦胚乳淀粉粒度分布特征及其对弱光的响应. 中国农业科学, 2008, 41(5): 1308-1316.

Cai R G, Yin Y P, Zhao F M, Zhang M, Zhang T B, Liang T B, Gu F, Dai Z M, Wang Z L. Size distribution of starch granules in strong-gluten wheat endosperm under low light environment. Scientia Agricultura Sinica, 2008, 41(5): 1308-1316. (in Chinese)

[28] Dai Z M, Starch granule size distribution in grains at different positions on the spike of wheat (Triticum aestivum L.). Starch/Stärke,2009, 61: 582-589.

[29] 戴忠民, 王振林, 高凤菊, 李文阳, 闫素辉, 蔡瑞国, 张敏, 尹燕枰. 两种供水条件下两穗型小麦品种籽粒淀粉积累及相关酶活性的变化特征. 作物学报, 2007, 33(4): 682-685.

Dai Z M, Wang Z L, Gao F J, Li W Y, Yan S H, Cai R G, Zhang M, Yin Y P. Characterization of starch accumulation and activities of enzymes involved in starch synthesis in grains of wheat cultivars differing in spike types field-grown in irrigation and rainfed conditions. Acta Agronomica Sinica, 2007, 33(4): 682-685. (in Chinese)

[30] 陈福泉, 张本山, 卢海凤, 赵永青, 张向阳. X射线衍射在淀粉颗粒结晶度研究中的应用. 食品科学, 2010, 31(3): 284-287.

Chen F Q, Zhang B S, Lu H F, Zhao Y Q, Zhang X Y. A review of application of X ray diffraction in crystal structure determination of starch granules. Food Science, 2010, 31(3): 284-287. (in Chinese)

[31] Cheetham N W H, Tao L. Variation in crystalline type with amylase content in maize starch granules: an X-ray powder diffraction study. Carbohydrate Polymer, 1998, 36: 277-284.

[32] Fujita S, Yamamoto H, Sugimoto Y, Morita N, Yamamori M. Thermal and crystalline properties of waxy wheat (Triticum aestivum L.)starch. Journal of Cereal Science, 1998, 27(1): 1-5.

[33] Myers A M, Morell M K, James M G, Ball S G. Recent progress toward understanding biosynthesis of the amylopectin crystal. Plant Physiology, 2000, 122: 989-997.

[34] 韩文芳, 熊善柏, 李江涛, 赵思明, 莫紫梅. 糯米淀粉的晶体性质和糊化特性. 中国粮油学报, 2015, 30(8): 48-53.

Han W F, Xiong S B, Li J T, Zhao S M, Mo Z M. The glutinous rice starch crystal properties and pasting properties. Journal of the Chinese Cereals and Oils Association, 2015, 30(8): 48-53. (in Chinese)

[35] 王晨阳, 苗建利, 张美微, 马冬云, 冯伟, 谢迎新, 郭天财. 高温、干旱及其互作对两个筋力小麦品种淀粉糊化特性的影响. 生态学报, 2014, 34(17): 4882-4890.

Wang C Y, Miao J L, Zhang M W, Ma D Y, Feng W, Xie Y X, Guo T C. Effects of post-anthesis high temperature, drought stress and their interaction on the starch pasting properties of two wheat cultivars with different gluten strength. Acta Ecologica Sinica, 2014, 34(17): 4882-4890. (in Chinese)

[36] 彭凯, 吴薇, 龙蕾, 王顺喜. 非淀粉成分对淀粉糊化特性的影响. 粮食与饲料工业, 2015, 12(5): 41-44.

Peng K, Wu W, Long L, Wang S X. Effect of non-starch components on the starch pasting properties. Cereal&Feed Industry, 2015, 12(5): 41-44. (in Chinese)

[37] Peterson D G, Fulcher R G. Variation in Minnesota HRS wheats: starch granule size distribution. Food Research International, 2001, 34(4): 357-363.

[38] Whattam J, Cornell H J. Distribution and composition of lipids in starch fractions from wheat flour. Starch-Stärke, 1991, 43(4): 152-156.

[39] 李春燕, 封超年, 王亚雷, 张容, 郭文善, 朱新开, 彭永欣. 不同小麦品种支链淀粉链长分配及其与淀粉理化特性的关系. 作物学报, 2007, 33(8): 1240-1245.

Li C Y, Feng C N, Wang Y L, Zhang R, Guo W S, Zhu X K, Peng Y X. Chain length distribution of debranched amylopectin and its relationship with physicochemical properties of starch in different wheat cultivars. Acta Agronomica Sinica, 2007, 33(8): 1240-1245. (in Chinese)

[40] Verwimp T, Vandeputte G E, Marrant K, Delcour J A. Isolation and characterisation of rye starch. Journal of Cereal Science, 2004, 39(1): 85-90.

[41] Mohamed A A, Rayas-Duarte P. The effect of mixing and wheat protein/gluten on the gelatinization of wheat starch. Food Chemistry, 2003, 81(4): 533-545.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effects of Drought Stress on Wheat Endosperm Starch Structure and Physicochemical Properties of Different Varieties

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN JiHao, ZHOU JieGuang, QU XiangRu, WANG SuRong, TANG HuaPing, JIANG Yun, TANG LiWei, $\boxed{\hbox{LAN XiuJin}}$, WEI YuMing, ZHOU JingZhong, MA Jian. Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat [J]. Scientia Agricultura Sinica, 2023, 56(2): 203-216.
[2]	YAN YanGe, ZHANG ShuiQin, LI YanTing, ZHAO BingQiang, YUAN Liang. Effects of Dextran Modified Urea on Winter Wheat Yield and Fate of Nitrogen Fertilizer [J]. Scientia Agricultura Sinica, 2023, 56(2): 287-299.
[3]	XU JiuKai, YUAN Liang, WEN YanChen, ZHANG ShuiQin, LI YanTing, LI HaiYan, ZHAO BingQiang. Nitrogen Fertilizer Replacement Value of Livestock Manure in the Winter Wheat Growing Season [J]. Scientia Agricultura Sinica, 2023, 56(2): 300-313.
[4]	HU Sheng,LI YangYang,TANG ZhangLin,LI JiaNa,QU CunMin,LIU LieZhao. Genome-Wide Association Analysis of the Changes in Oil Content and Protein Content Under Drought Stress in Brassica napus L. [J]. Scientia Agricultura Sinica, 2023, 56(1): 17-30.
[5]	ZHAO HaiXia,XIAO Xin,DONG QiXin,WU HuaLa,LI ChengLei,WU Qi. Optimization of Callus Genetic Transformation System and Its Application in FtCHS1 Overexpression in Tartary Buckwheat [J]. Scientia Agricultura Sinica, 2022, 55(9): 1723-1734.
[6]	WANG HaoLin,MA Yue,LI YongHua,LI Chao,ZHAO MingQin,YUAN AiJing,QIU WeiHong,HE Gang,SHI Mei,WANG ZhaoHui. Optimal Management of Phosphorus Fertilization Based on the Yield and Grain Manganese Concentration of Wheat [J]. Scientia Agricultura Sinica, 2022, 55(9): 1800-1810.
[7]	TANG HuaPing,CHEN HuangXin,LI Cong,GOU LuLu,TAN Cui,MU Yang,TANG LiWei,LAN XiuJin,WEI YuMing,MA Jian. Unconditional and Conditional QTL Analysis of Wheat Spike Length in Common Wheat Based on 55K SNP Array [J]. Scientia Agricultura Sinica, 2022, 55(8): 1492-1502.
[8]	MA XiaoYan,YANG Yu,HUANG DongLin,WANG ZhaoHui,GAO YaJun,LI YongGang,LÜ Hui. Annual Nutrients Balance and Economic Return Analysis of Wheat with Fertilizers Reduction and Different Rotations [J]. Scientia Agricultura Sinica, 2022, 55(8): 1589-1603.
[9]	LIU Shuo,ZHANG Hui,GAO ZhiYuan,XU JiLi,TIAN Hui. Genetic Variations of Potassium Harvest Index in 437 Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(7): 1284-1300.
[10]	WANG YangYang,LIU WanDai,HE Li,REN DeChao,DUAN JianZhao,HU Xin,GUO TianCai,WANG YongHua,FENG Wei. Evaluation of Low Temperature Freezing Injury in Winter Wheat and Difference Analysis of Water Effect Based on Multivariate Statistical Analysis [J]. Scientia Agricultura Sinica, 2022, 55(7): 1301-1318.
[11]	GOU ZhiWen,YIN Wen,CHAI Qiang,FAN ZhiLong,HU FaLong,ZHAO Cai,YU AiZhong,FAN Hong. Analysis of Sustainability of Multiple Cropping Green Manure in Wheat-Maize Intercropping After Wheat Harvested in Arid Irrigation Areas [J]. Scientia Agricultura Sinica, 2022, 55(7): 1319-1331.
[12]	ZHANG JiaHua,YANG HengShan,ZHANG YuQin,LI CongFeng,ZHANG RuiFu,TAI JiCheng,ZHOU YangChen. Effects of Different Drip Irrigation Modes on Starch Accumulation and Activities of Starch Synthesis-Related Enzyme of Spring Maize Grain in Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1332-1345.
[13]	ZHI Lei,ZHE Li,SUN NanNan,YANG Yang,Dauren Serikbay,JIA HanZhong,HU YinGang,CHEN Liang. Genome-Wide Association Analysis of Lead Tolerance in Wheat at Seedling Stage [J]. Scientia Agricultura Sinica, 2022, 55(6): 1064-1081.
[14]	QIN YuQing,CHENG HongBo,CHAI YuWei,MA JianTao,LI Rui,LI YaWei,CHANG Lei,CHAI ShouXi. Increasing Effects of Wheat Yield Under Mulching Cultivation in Northern of China: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(6): 1095-1109.
[15]	CAI WeiDi,ZHANG Yu,LIU HaiYan,ZHENG HengBiao,CHENG Tao,TIAN YongChao,ZHU Yan,CAO WeiXing,YAO Xia. Early Detection on Wheat Canopy Powdery Mildew with Hyperspectral Imaging [J]. Scientia Agricultura Sinica, 2022, 55(6): 1110-1126.