小麦-长穗偃麦草7E抗赤霉病易位系培育

doi:10.3864/j.issn.0578-1752.2016.18.002

Abstract

Abstract: 【Objective】The objective of this study was to transfer the chromosome 7E of Thinopyrum elongatum into cultivated common wheat (Triticum aestivam L.) to develop translocation lines resistant to Fusarium Head Blight (FHB) and thereby to provide new germplasm for improving FHB resistance in common wheat.【Method】The F₂ seeds from the cross between Yangmai16 and DS7E(7B) were radiated using ⁶⁰Co (at 30 000 rad), DS7E(7B) being a substitution line in which the chromosome 7B of Chinese Spring common wheat was substituted with the chromosome 7E of Th. elongatum. The survived M₁plants were harvested, after visual selection for agronomic traits. The M₂ to M₄ populations were selected for agronomic traits, FHB resistance under single drop injection with Fusarium graminearum, and molecular markers specific to the chromosome and chromosomal arm of 7E for Th. elongatum, followed by cytological confirmation for the presence of 7E chromosomes using Th. elongatum genomic DNA as probe by genomic in situ hybridization (GISH). 【Result】Thirteen M₁ plants with varying degrees of FHB resistance were selected, and the corresponding M₂ plants were examined for the presence of previously developed molecular markers specific to chromosome 7E . Seven plants were found to carry the long arm of 7E and 14 were found to carry the short arm of 7E. After selfing, 13 plants carrying markers specific to the long arm of 7E chromosome and 3 plants carrying markers specific to the short arm of 7E chromosome were identified out of 223 M₃ plants. GISH analysis was conducted for the progenies (M₄) derived from 12 M₃ plants and it was found that the progenies from nine of the M₃ lines were wheat-Th. elongatum translocation lines (2n=42), and those from two other M₃ plants were chromosome addition lines with the short arm of 7E (2n=44). Continued selection led to the development of three translocation lines carrying the long arm of 7E, which were named as TW-7EL1, TW-7EL2 and TW-7EL3, respectively. A fourth line was a chromosome addition line with the short arm of 7E and was named as W-DA7ES. These four lines were derived from two different M₁ plants. Evaluation of FHB resistance indicated that the translation lines were similar to Sumai 3 in FHB resistance, better than Chinese Spring and Yangmai 16, while the addition line was considerably poorer in FHB resistance.【Conclusion】Translocation lines with chromosome 7EL that are resistant to FHB were developed effectively and accurately by joint use of phenotypic selection, screening for chromosome-specific molecular markers to 7E, and genomic in situ hybridization. The chromosome 7EL of Th.elongatum carries FHB-resistant genes.

Key words: wheat, Thinopyrum elongatum, fusarium head blight, translocation lines

ZHANG Lu-lu, CHEN Shi-qiang, LI Hai-feng, LIU Hui-ping, DAI Yi, GAO Yong, CHEN Jian-min. Development of Wheat -Thinopyrum elongatum Translocation Lines Resistant to Fusarium Head Blight[J].Scientia Agricultura Sinica, 2016, 49(18): 3477-3488.

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