Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (11): 2153-2163.doi: 10.3864/j.issn.0578-1752.2016.11.011

• HORTICULTURE • Previous Articles     Next Articles

Effects of Fusarium Oxysporum f. sp. melonis on Lignin, Activities of Lingin-Related Enzymes and Genes Expressions of CmCADs in Oriental Melon (Cucumis melo var. makuwa Makino)

LIU He-juan, LI Yue-peng, LIU Wei, SHAO Qi, QI Hong-yan   

  1. College of Horticulture, Shenyang Agricultural University/Key Laboratory of Protected Horticulture, Ministry of Education/ Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region, Shenyang 110866
  • Received:2015-10-12 Online:2016-06-01 Published:2016-06-01

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Abstract: 【Objective】The purpose of this study is to investigate the effects of Fusarium oxysporum on symptom, lignin content, activities of lignin-related enzymes and real-time fluorescent quantitative PCR analysis of CmCADs gene expression of CmCADs in oriental melon seedlings and to find out the gene family members of CmCADs that can respond to the fusarium oxysporum. 【Method】The oriental melon ‘Caihong 7’ which is resistant to fusarium oxysporum was used as experimental materials. Then the melon seedlings were inoculated with F. oxysporum at 3 true leaves stage. The control plants were dealt with equivalent sterile water. The changes of phenotype were observed and lignin content, activities of lingin-related enzymes (PAL, POD and CAD) and genes expressions of CmCADs in the vegetative organs of oriental melon seedlings were determined at 0, 1, 3, 5, 7 and 9 days after inoculation.【Result】The symptom of oriental melon seedlings showed obvious changes. The leaves began to wilt slightly 5 days after inoculation and wilt badly 9 days after inoculation. The results showed that compared with the control, the lignin content and the activities of lignin-related enzymes were all increased to a certain extent. The change trend of lignin content in roots, stems and leaves was basically identical with keeping increasing and higher than the control treatment. The activities of PAL which increased first and then decreased in roots and stems while exhibited a global increased trend in the leaves were obviously higher than the control treatment. The activities of POD presented different changes with higher activities than control in each period. The activities of CAD presented a trend of earlier increase and later decrease in roots, stems and leaves. In the study, CmCADs showed tissue-specific expression after inoculation. CmCAD1, CmCAD 2, CmCAD 3 and CmCAD 5 were strongly induced in roots 7 days after inoculation, except CmCAD4, while only CmCAD2 and CmCAD5 in leaves were induced strongly 5 days after inoculation. No CmCADs expressions were observed in stems of melon. 【Conclusion】 It was found that the increase of the lignin content, activities of lignin-related enzymes (PAL, POD, CAD) caused by F. oxysporum suggested that the lignin biosynthesis pathway may play an important role in defending the oriental melon from the F. oxysporum. It was also inferred that CmCAD2 and CmCAD5 which were both induced by F. oxysporum may the members of CmCADs which can response to the F. oxysporum and play a role in resistance to F. oxysporum in oriental melon.

Key words: oriental melon; Fusarium oxysporum f. sp. Melonis, lignin, CAD, genes expressions

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