Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (24): 4989-4995.doi: 10.3864/j.issn.0578-1752.2015.24.013

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Study on Expression of Glutathione Peroxidase type-5 (GPX5)   and Protein Localization in Epididymis of Adult Sheep

LI Rui-lan, ZHANG Tong, FAN Xiao-mei, OU YANG Xiao-qing, ZHANG Chun-qiang, CAO Jun-wei, ZHANG Jia-xin   

  1. Key Laboratory of Animal Genetics，Breeding and Reproduction of Inner Mongolia Autonomous Region/The College of Animal Science, Inner Mongolia Agricultural University，Hohhot 010018
  • Received:2015-01-20 Online:2015-12-16 Published:2015-12-16

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Abstract: 【Objective】 The objective of this study was to investigate the expression pattern of GPX5（Glutathione peroxidase type-5）and GPX5 protein localization in adult sheep epididymis to provide a theoretical basis for studying antioxidative mechanisms of sperm in sheep epididymis. 【Method】 Epididymis, testis and deferent duct of adult Mongolia sheep of similar age were collected. The epididymis of each ram were selected as epididymal caput, epididymal corpus and epididymal cauda, respectively. Samples were stored at -80 ℃. The expression of GPX5 in epididymis, testis and deferent duct were detected by real-time fluorescent quantitative PCR and Western blot. The fresh tissue samples of different segments were cut to suitable size and immersed in 4% paraformaldehyde solution for 24 hours; then paraffin embedded tissue sections were made and incubated with GPX5 specific antibody. The positive signals of GPX5 protein were detected by DAB kit. 【Result】The result of real-time fluorescent quantitative PCR showed that the expression level of GPX5 of caput was significantly higher than that of corpus and cauda (P＜0.01), but almost no expression were found in testis or deferent duct. We showed that GPX5 protein was highly expressed in caput, and a small amount of GPX5 protein was present in corpus and cauda; however no GPX5 protein was found in testis nor deferent duct. Collectively, these studies suggested that GPX5 expressed mainly in the epididymal caput of adult sheep. As assessed by immunohistochemical staining, the GPX5 protein was localized mainly in the epithelium and stereocilia of caput, while in corpus and cauda the GPX5 protein localized mainly in stereocilia. The GPX5 protein was detected in sperm located in caput, corpus, cauda as well as epididymal lumen. The GPX5 protein secreted from epididymis epithelium into epididymal lumen and combined gradually with spermatozoa during epididymal transit. 【Conclusion】GPX5 was synthesized and secreted mostly in the epididymal caput epithelium of adult sheep and combined with spermatozoa in epididymal lumen, thereby providing protection for their normal physiological functions.

Key words: sheep, epididymis, GPX5, genetic expression, protein localization

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