Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (18): 3666-3675.doi: 10.3864/j.issn.0578-1752.2015.18.010

• HORTICULTURE • Previous Articles     Next Articles

Genetic Analysis and QTL Mapping of Wax Powder on the Surface of Cucumber Fruit

TIAN Gui-li, ZHANG Sheng-ping, SONG Zi-chao, ZHANG Song, CUI Jin-ying, MIAO Han, GU Xing-fang   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2015-03-15 Online:2015-09-16 Published:2015-09-16

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Abstract: 【Objective】Cucumber (Cucumis sativus L.) is an important world vegetable in the world and its output was over  65 000 million kilos in 2012. For many years, plant breeders have focused their attentions on improving fruit quality, especially in flavor, nutrition, and appearance of cucumber. Several researches on the traits to affecting the appearance quality of cucumber fruit have been reported. However, up to now the amount of wax on the surface of the fruit has received little attention. Wax powder is one of the important fruit appearance quality traits in cucumber. The inheritance and QTL mapping will help to understand the molecular mechanism of wax powder formation, lay a foundation for the fine mapping and gene cloning, and provide a theoretical basis for selecting cucumber lines with little wax powder.【Method】In this study, six generations were developed from the cross between cucumber lines with heavy wax powder ‘PI183697’ and light wax powder ‘1101’ under different environmental conditions of Hainan and Beijing. The amount of wax powder on the fruit surface was measured quantitatively with colour meter (CM-700 d).2-3 cucumbers in each individual and five parts on each cucumber were selected for measurement, and then the calculated average values were used to estimate the inheritance. For the genetic analysis, 1 288 SSR markers were tested and 128 that were found to be polymorphic between ‘PI183697’ and ‘1101’ were used for linkage analysis of F2 populations. A linkage group was constructed by using JoinMap4.0 with minimum LOD 3.0, and QTL detection for wax powder was conducted with MapQTL4.0.【Result】Genetic analysis showed that the trait of wax powder in ‘PI183697’ was inherited quantitatively. The mixed major gene plus polygene inheritance model of plant quantitative traits was applied to the six generations for genetic analysis. The result showed that the trait of wax powder on cucumber fruit surface was controlled by one additive major gene plus additive-dominant polygene (D-2 model) in the joint analysis of six generations. Linkage maps from two F2 populations containing seven chromosomes and 128 SSR markers were constructed and seven QTLs relating to wax powder were identified. One QTL locus on Chr.1, Chr.3 and Chr.5, respectively, and four loci on Chr.6 were detected, which were WP1.1, WP3.1, WP5.1, WP6.1, WP6.2, WP6.3 and WP6.4. The QTL loci WP5.1 and WP6.2 were detected in two seasons, having LOD scores of 7.70, 4.81, 4.21, and 6.69, and R2 of 14.9%, 12.4%, 8.0% and 16.7%, respectively. 【Conclusion】The inheritance of wax powder is quantitative and controlled by one additive major gene plus additive-dominant polygene (D-2 model). According to QTL mapping, the QTL loci WP5.1 and WP6.2 were repeatedly detected. Thus it was predicted that the major QTL loci may be located on Chr.5 and Chr.6.

Key words: cucumber (Cucumis sativus L.), wax powder, linkage map, QTL

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