Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (14): 2777-2784.doi: 10.3864/j.issn.0578-1752.2015.14.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Dynamic Kinetic Characteristics of Different Ratios of Ammonium and Nitrate Absorbed by Banana Seedlings

ZHANG Chao-yi, FAN Xiao-lin   

  1. College of Natural Resources and Environmental Science, South China Agricultural University/Guangdong Province Research Center of Environmental Friendly Fertilizer Engineering & Technology, Guangzhou 510642
  • Received:2014-12-17 Online:2015-07-16 Published:2015-07-16

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Abstract: 【Objective】Ammonium and nitrate and their interaction effect on N absorption by banana seedlings were studied to screen the optimum ratio of ammonium to nitrate for banana growth and efficient utilization of nitrogen. 【Method】 The NH4+-N, NO3--N and total nitrogen kinetics of N uptake by banana seedlings were studied by modified depletion method based on the principle of nutrient absorption kinetics in the solution culture with different ratios of ammonium to nitrate. 【Result】 Michaelis-Menten enzyme kinetic model or equation could be applied to describe the patterns of absorption of NH4+-N, NO3--N and total N by banana seedlings in all the treatments and the co-relationship coefficient r was at a significant level. When the rate of NH4+-N was at 10% and 70%, NH4+-N absorption rate increased with the NO3--N addition gradually. The maximum absorption rate (Vmax) of NH4+-N uptake reached the maximum of 55.56 μmol·g-1·h-1, when the NH4+-N ratio increased to 70%. The NH4+-N absorption rate decreased again when the NH4+-N ratio was more than 70%. However, the rate of nitrate absorption of banana declined with the increase of NH4+-N. When the proportion of NH4+-N increased from 10% to 90%, the Vmax of NO3--N decreased by 2.62 times. Therefore, adding NH4+-N would significantly inhibit the banana seedlings to absorb NO3--N. There was no obvious effect of the ratio of ammonium to nitrate on affinity of banana root to NH4+-N and NO3--N. When ammonium to nitrate ratio was 3﹕7, the total nitrogen absorption rate was 83.33 μmol·g-1·h-1, being the maximum rate and in favor of banana to take up nitrogen. 【Conclusion】 When the proportion of NH4+-N was lower than 70%, increase of NO3--N could enhance the banana seedlings to take up NH4+-N. When the ammonium ratio was more than 70%, increase of NO3--N would inhibit the banana seedlings to take up NH4+-N. Increasing ratio of NH4+-N could significantly inhibit the banana seedlings to absorb NO3--N. The optimum ratio of ammonium to nitrate being favor of nitrogen absorption was 3﹕7.

Key words: banana, NH4+-N, NO3--N, nitrogen uptake, absorption kinetics

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