玉米大斑病菌黑色素合成酶基因的全基因组定位及表达模式分析

doi:10.3864/j.issn.0578-1752.2015.14.008

Abstract

Abstract: 【Objective】The objectives of this study are to determine the location in the genome, and to investigate the structure of melanin biosynthesis genes StPKS, StSCD, St3HNR, St4HNR, StLAC1 and StLAC2 of Setosphaeria turcica. Furthermore, to elucidate the relationship between melanin biosynthesis enzyme genes and morphogenesis and pathogenicity of S. turcica viaanalyzing the expression pattern of six genes during infection progress from conidial germination to penetration and the mycelia growth period.【Method】 Based on the S. turcica genome database and genome searching with Blastp, the locations of six genes were identified and genes linkage were analyzed. Primers were designed according to the six melanin synthesis enzyme gene sequences. qRT-PCR system was used to detect the six genes expressions of S. turcica, in which total RNAs from different infectious morphogenesis stages including conidial germination to penetration were extracted and used as templates and β-tubulin as the reference gene, and the expression patterns between vegetative and reproductive growth stages were compared.【Result】In genome database, the StPKS and St3HNR were located on the positive and negative strand of scaffold_12, respectively. St4HNR and StLAC2 were on the negative strand scaffold_11. StSCD and StLAC1 located on the positive of strand scaffold_1 and scaffold_7, respectively. Furthermore, StPKS and St3HNR were clustered within the 26.9 kb chromosomal region. Relative expression of six genes had two expression profiles, which was up-down- up regulation or up- down- up- down regulation at five stages from conidial germination to penetration. The StLAC2 had the highest expression in conidia, and there was a significant difference in expression. However, St3HNR and StSCD had a higher expression level than other genes in mycelia.【Conclusion】StPKS and St3HNR in melanin biosynthesis were clustered within a 26.9 kb chromosomal region. Six genes were whole expressed at five stages from conidial germination to penetration. They had a similar expression pattern. Unfortunately, they had a significant difference in expression levels. The results showed that six genes of melanin biosynthesis enzyme participated in the infection process and played an important role in the pathogenic of S. turcica. In addition, St3HNR and StSCD played an important role at mycelial stage. StLAC2 had the highest expression at conidial stage.

Key words: Setosphaeria turcica; melanin, biosynthesis enzyme gene, location, expression pattern

JIA Hui, MENG Qing-jiang, LI Zhi-yong, GONG Xiao-dong, ZANG Jin-ping, HAO Zhi-min, CAO Zhi-yan, DONG Jin-gao. Localization of Melanin Biosynthesis Enzyme Genes in the Genome and Expression Pattern Analysis of Setosphaeria turcica[J].Scientia Agricultura Sinica, 2015, 48(14): 2767-2776.

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Localization of Melanin Biosynthesis Enzyme Genes in the Genome and Expression Pattern Analysis of Setosphaeria turcica

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