Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (12): 2408-2416.doi: 10.3864/j.issn.0578-1752.2015.12.014

• HORTICULTURE • Previous Articles     Next Articles

The Molecular Mechanism of UV-A Induced Anthocyanin Accumulation in the Hypocotyls of Soybean Sprouts

QI Nan-nan, ZHANG Xiao-yan, SU Na-na, WU Qi, GENG Dian-xiang, QI Xue-hui, WEI Sheng-jun, CUI Jin   

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095
  • Received:2015-01-24 Online:2015-06-16 Published:2015-06-16

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Abstract: 【Objective】The objective of the experiment was to investigate the effect of UV-A, as well as white (W) and blue (B) lights on content of anthocyanin, activities of related enzymes, transcript levels of anthocyanin biosynthesis-related genes and light receptor genes. This study would find out the molecular mechanism about UV-A induces anthocyanin accumulation in the hypocotyls of soybean sprouts and provide a theoretical foundation for the application of light quality control technology in the industrialized production of soybean sprouts.【Method】The seedlings of soybean variety ‘Dongnong 690’ were treated with continuous UV-A, white light (W) and blue light (B). Samples were collected at 0, 12, 24 and 36 h after treatment, respectively. The anthocyanin content, the activities of PAL, CHI, and UFGT and the expression of related genes (PAL, CHS, CHI, DFR, ANS, UFGT, MYB75, CRY1, CRY2 and UVR8) were detected. The difference of anthocyanin content, related enzymes and genes expression under different light qualities in the hypocotyls of soybean sprouts was analyzed. The anthocyanin content was determined by spectrophotometric method, phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) activities were measured by spectrophotometry, flavonoid galactosyl transferase (UFGT) activity by ultra performance liquid chromatography (UPLC) determination. Total RNA was extracted using the Trizol reagent. Gene expression was determined by qRT-PCR.【Result】Soybean sprouts grown in the dark showed yellow cotyledons while sprouts grown under white (W), blue (B) and UV-A lights existed green cotyledons. Compared with the dark and other light quality cultures, UV-A treatment significantly improved the content of anthocyanin in the hypocotyls of soybean sprouts. As the illumination time goes on, anthocyanin accumulation was increased gradually. The anthocyanin content in the hypocotyls of soybean sprouts was low at 0 h, about 2 U·g-1 FW. The content of anthocyanin reached a maximum of 43 U·g-1 FW at 36 h under UV-A, which was significantly higher than those in the other treatments. The activities of PAL and CHI were higher at 0 h. Compared to that in the dark and other light treatments, continuous UV-A light treatment for 24 h and 36 h significantly increased the activity of phenylalanine ammonia-lyase (PAL). Continuous UV-A light treatment for 12 h and 24 h significantly increased the activity of and flavonoid glycosyltransferase (UFGT). There was no difference in the expression of anthocyanin related genes among different treatments at 0 h. Continuous UV-A treatment for 36 h significantly up-regulated the expression of regulator gene MYB75 and light receptor genes (CRY1, CRY2 and UVR8) to about 12-, 30-, 6- and 2-fold, in the hypocotyls of soybean sprouts compared with those in the other treatments, respectively. Moreover, continuous UV-A treatment for 12 h significantly up-regulated the expression of anthocyanin biosynthesis-related structural genes (PAL, CHS, DFR, ANS, UFGT) to about 5, 58, 10, 6, 44 and 47 times, respectively.【Conclusion】UV-A induced anthocyanin accumulation by improving the activities of PAL and UFGT and up regulating the expression of related genes in the hypocotyls and light receptor of soybean sprouts.

Key words:  soybean sprouts, anthocyanin, UV-A, molecular mechanism

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