Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (5): 851-860.doi: 10.3864/j.issn.0578-1752.2015.05.03

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Molecular Characteristics and Functional Identification of Foxtail Millet Transcription Factor WRKY36

ZU Qian-li1,2, YIN Li-juan2, XU Zhao-shi2, CHEN Ming2, ZHOU Yong-bin1,2, LI Lian-cheng2, MA You-zhi2, MIN Dong-hong1, ZHANG Xiao-hong1   

  1. 1 Northwest A & F University/State Key Laboratory of Crop Stress Biology for Arid Areas , Yangling 712100, Shaanxi
    2 Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement/ Key Laboratory of Biology and Genetic Improvement of Triticeae Crop, Ministry of Agriculture, Beijing 100081
  • Received:2014-09-19 Online:2015-03-01 Published:2015-03-01

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Abstract: 【Objective】 Abiotic stresses seriously affect the plant growth development and the crop yield. WRKY transcription factors play a key role in the regulatory mechanism. This study provided experimental data for further study of the molecular characteristics and the function of SiWRKY36 gene. 【Method】 SiWRKY36 gene was isolated from the drought-treated foxtail millet transcriptome profile. Bioinformatics methods were used to analyze the molecular properties of the SiWRKY36 gene. Homologous sequences of SiWRKY36 were selected from the Phytozome program. Homologous analysis and multiple alignments were performed with MEGA 5. MEME and SMART online tools were used for protein sequence analysis. GSDS and PHYRE2 online tools were used to analyze gene structure and tertiary structure of SiWRKY36, respectively. Quantitative real-time PCR (qRT-PCR) was used to examine the expression pattern of SiWRKY36 gene in different abiotic stresses and phytohormone treatments. Full length SiWRKY36 cDNA was ligated into the vector to construct an expression vector for wild-type Arabidopsis plant. The pBI121-SiWRKY36 expression vector was introduced into Agrobacterium tumefaciens strain cells. Transgenic plants were used for resistance identification experiment. 【Result】 SiWRKY36 shared a high similarity with switchgrass and belonged to Group Ι of WRKY family including two WRKYGQK conserved domains. The predicted tertiary structure of SiWRKY36 contained two alpha helix and three beta folds. SiWRKY36 was mainly located in nucleus. The expression pattern showed that SiWRKY36 was involved in responses to various abiotic stresses and exogenous hormones, which was similar with the promoter analysis. The total root length, root surface area and root volume were slightly different between three SiWRKY36 lines and wild-type plants in drought treatment (2% PEG) in Arabidopsis plants. 【Conclusion】 SiWRKY36 transgenic plants have a certain resistance under mild drought conditions.

Key words: Foxtail millet, WRKY transcription factor, stress responses, subcellular localization, stress resistance

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