Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (23): 4858-4867.doi: 10.3864/j.issn.0578-1752.2013.23.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Development and Characterization of Both PvPGIP2 and TaLTP5 Transgenic Wheat Lines with Resistance to Take-all

 RONG  Wei-12, WANG  Jin-Feng-2, LI  Zhao-2, WANG  Ai-Yun-1, DU  Li-Pu-2, YE  Xing-Guo-2, WEI  Xue-Ning-2, ZHANG  Zeng-Yan-2   

  1. 1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004
    2.Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing 100081
  • Received:2013-05-28 Online:2013-12-01 Published:2013-09-06

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Abstract: 【Objective】 Take-all is a destructive disease of wheat production worldwide. The aim of this study is to develop and select stable PvPGIP2 and TaLTP5 transgenic wheat lines with resistance to take-all pathogen Gaeumannomyces graminis. 【Method】Gene recombination technology was used to prepare the transformation vector pA25-PvPGIP2-TaLTP5 expressing both PvPGIP2 and TaLTP5. Particle bombardment method was used to introduce both PvPGIP2 and TaLTP5 into wheat cultivar Yangmai 18. PCR, RT-PCR and qRT-PCR methods were used to detect the presence and transcript levels of PvPGIP2 and TaLTP5 in the transgenic wheat plants of T0-T4 generations. G. graminis mycelia plug inoculation and take-all severity and the disease index were used to score the resistance degrees of these transgenic wheat lines and non-transgenic wheat Yangmai 18 at young seedling-stage. 【Result】The results indicated that the introduced PvPGIP2 and TaLTP5 genes were stably inherited, and could be highly expressed in six transgenic wheat lines. The transgenic wheat plants expressing PvPGIP2 and TaLTP5 showed significantly enhanced resistance to G. graminis compared with non-transgenic wheat Yangmai 18. 【Conclusion】 These results suggested that the introduced PvPGIP2 and TaLTP5 genes can be used for improving wheat resistance to take-all.

Key words: wheat , PvPGIP2 , TaLTP5 , binary tansgene , take-all , transgenic

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