Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4586-4593.doi: 10.3864/j.issn.0578-1752.2013.21.022

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Characterization, Expression of TGF-β1 Gene and Its Association with Ovulation Rate in Hu Sheep

 GUO  Jing-1, LI  Xin-Yu-1, LI  Yin-Xia-2, PAN  Zeng-Xiang-1, JUE  Ken-3, LIU  Ji-Ying-1, LI  二Lin-1, XIE  Zhuang-1, CHEN  Ling-4, LI  Qi-Fa-1   

  1. 1.College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095;
    2.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014;
    3.College of Animal Science, Xinjiang Agricultural University, Urumqi 830052;
    4.Suzhou Breeding Sheep Farm of Jiangsu Province, Suzhou 215200, Jiangsu
  • Received:2013-04-25 Online:2013-11-01 Published:2013-06-17

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Abstract: 【Objective】 The present study was performed to clone the TGF-β1 sequence in Hu sheep and to comprehend the sequence character of Hu sheep so as to investigate the relationships between the mRNA level of TGF-β1 gene and ovulation number in ovaries of Hu sheep. 【Method】The sequence of TGF-β1 in Hu sheep was cloned and sequenced, and the sequence was analyzed by bioinformatics. The tissue distributions of TGF-β1 were detected by RT-PCR, and the gene expression level differences in the ovaries between the high- and the low-fecundity groups and the relationships with the ovulation rate were detected by real-time qPCR.【Result】The length of TGF-β1 gene coding sequence was 1 173 bp, encoding 390 amino acids which include some typical domains such as TGF-β propeptide and TGF-β like. RT-PCR assays revealed that TGF-β1 highly expressed in the ovary tissue. The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group (P＜0.05), and mRNA expression level was significantly positively correlated with ovulation rate (P＜0.01). 【Conclusion】The ovarian mRNA expression level of TGF-β1 in high-fecundity group was significantly greater than that in low-fecundity group and significantly positively correlated with ovulation rate. These findings indicate that TGF-β1 of Hu sheep play an important role in follicular development, maturation or ovulation, which probably is associated with high fecundity of Hu sheep.

Key words: Hu sheep , TGF-β1 gene , litter size , ovulation rate

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