Smads与Hippo通道中YAP1基因在湖羊肌肉组织中时空表达研究及关联分析

doi:10.3864/j.issn.0578-1752.2016.11.016

Abstract

Abstract: 【Objective】 In order to explore the expression pattern and inner connection between the different genes, the mRNA expression level was used to detect the spatial and temporal expression of genes in TGF-β/Smad signaling pathway of sheep muscle tissue. 【Method】 In this study, q-PCR was used to detect relative expression of Smads genes and Yes-associated protein 1(YAP1) in 2 different skeletal muscles (i.e., gastrocnemius muscle, extensor digitorum longus) and 3 different growth stages (i.e., 2-day-old, 2 and 6-month-old) of Hu Sheep, and analyze the relative expression between Smads(Smad2, Smad3, Smad4 and Smad7) and YAP1. 【Result】The results of the spatial and temporal expression of Smads in TGF-β/Smad signaling pathway showed that the expression of Smads in extensor digitorum longus were lower than in gastrocnemius muscle, which may be related to two different parts of the skeletal muscle. The expression of Smad2, Smad3 and Smad4 genes in the 2-day-old were higher than other growth stages, and Smad7 gene expression in the 2-day-old was lower than 6-month-old, and the expression in 2-month-old was the lowest. The expressions of Smads gene of ram in the 2-day-old was higher than ewe, and the expressions of Smad2, Smad4, and Smad7 genes of ram in 2-month-old and 6-month-old were lower than the ewe in 2-day-old, the expression of Smad3 gene of ram in 3 growth stages were higher than ewe. The correlation expression of Smads gene and YAP1 gene in TGF-β/smad signaling of sheep muscle was found that there was no significant correlation in 2-day-old of gastrocnemius muscle between YAP1 and smads (P＞0.05) . The expression between YAP1 and Smad2 had a significant positive correlation (P＜0.05) and the correlation of the expression between YAP1 and other Smads was not significant in 2-month-old of gastrocnemius muscle (P＞0.05). There was a significant negative correlation between YAP1 and Smad3 in different growth stages of gastrocnemius muscle(P＜0.01). YAP1 expression exhibited a high positive correlation with Smad3 and the expression of YAP1 with the others was significantiy correlated in extensor digitorum longus of 2-month-old (P＜0.05). There was no significant correlation between YAP1 and Smads in 2-month-old of extensor digitorum longus (P＞0.05). The expression between YAP1 and Smad7 had a high significant positive correlation (P＜0.01) and the correlation of expression between YAP1 and other Smads was not significant in 6-month-old of extensor digitorum longus (P＞0.05). There were highly significant negative correlations between YAP1 and Smads in different growth stages of extensor digitorum longus (P＜0.01). 【Conclusion】 It was suggested that different tissues, growth stages and gender could affect the expression of Smads in muscle. YAP1 of Hippo pathway may participate in regulating the TGF-β/smad pathway and the process of muscle proliferation and differentiation in gastrocnemius muscle and extensor digitorum longus of Hu sheep.

Key words: Hu sheep, Smads, YAP1, Hippo pathway, TGF-&beta, pathway

BAO Jian-jun, SU Rui, WANG Qing-zeng, Lü Xiao-yang, GAO Wen, YU Jia-rui, WANG Li-hong, CHEN Ling, WU Wen-zhong, SHENG Shui-xing, ZHOU Hong, SUN Wei, DAI Guo-jun. Study on the Temporal and Spatial Expression and Correlation Analysis of Smads and YAP1 Gene in the Hippo Pathway in Sheep Muscle Tissue[J].Scientia Agricultura Sinica, 2016, 49(11): 2203-2213.

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Study on the Temporal and Spatial Expression and Correlation Analysis of Smads and YAP1 Gene in the Hippo Pathway in Sheep Muscle Tissue

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