Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4594-4602.doi: 10.3864/j.issn.0578-1752.2013.21.023

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Cloning and Identification of Arylphorin from Silkworm Infected by Cytoplasmic Polyhedrosis Virus

 GAO  Kun-123, DENG  Xiang-Yuan-1, WU  Ping-23, QIN  Guang-Xing-23, HOU  Cheng-Xiang-23, QIAN  He-Ying-23, GENG  Tao-23, GUO  Xi-Jie-23   

  1. 1.College of Biotechnology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu
    2.Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu
    3.Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu
  • Received:2013-04-07 Online:2013-11-01 Published:2013-07-10

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Abstract: 【Objective】 The objective of this study is to clone the full-length cDNA sequence of Arylphorin in silkworm (Bombyx mori) to analyze the gene sequence by bioinformatics and to investigate the mRNA expression level after the B. mori infected by cytoplasmic polyhedrosis virus (BmCPV), and to provide a new foundation for the further study of its biological function. 【Method】 Rapid ampli?cation of cDNA ends (RACE) approach was employed to clone the full-length cDNA of Arylphorin from the total RNA of the silkworm midgut. Fluorescent quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression profiling of Arylphorin in different tissues and at different time points after the BmCPV infection. 【Result】 The full-length cDNA of Arylphorin was obtained and named as BmAryl with accession number of JN581664 in GenBank. It consisted of a 5′-terminal untranslated region (UTR) of 26 bp and a 3′-UTR of 143 bp with polyadenylation signal sequences AATAAA and a poly (A) tail. The longest open reading frame (ORF) encoded a polypeptide of 696 amino acids with a theoretical isoelectric point of 6.07 and the predicted molecular weight of 82.8 kD. Sequence comparison showed that BmAryl was 66% identical to B. mori sex-specific storage-protein 2 precursor. mRNA transcripts of BmAryl were detected mainly in fat body and hemolymph and the expression level had no difference between the testicle and ovary. For the silkworm larvae infected by BmCPV, the relative expression level of BmAryl was signi?cantly down-regulated in the midgut. 【Conclusion】 The full-length cDNA of BmAryl was successfully cloned from silkworm, which encodes a protein that belongs to the aromatic protein family. BmAryl expression were detected mainly in fat body and hemolymph, not sex-specific but down-regulated in the midgut due to BmCPV infection. These results has provided not only helpful information for further studying the function of BmAryl in silkworm but also be helpful for understanding of the role in response to BmCPV infection.

Key words: Bombyx mori , cytoplasmic polyhedrosis virus , Arylphorin , midgut

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