湖羊不同花纹皮肤组织差异表达基因的筛选

doi:10.3864/j.issn.0578-1752.2013.02.017

Abstract

Abstract: 【Objective】The molecular genetic mechanism of China’s unique white lambskin breed different flowers patterns of Hu sheep and hair follicle formation were studied.【Method】The article focused on screening differentially expressed genes of new born Hu-sheep’s skin tissue by Agilent gene chip.【Result】 Differential expression analysis revealed that 1067, 2071 and 3879 differentially expressed genes could be screened from 3 groups of large flowers and small flowers, and compared the 3 groups it could find that there were 137 genes in common. Differentially expressed genes classified by GO (Gene Ontology) analysis mainly involved in cell differentiation, proliferation, apoptosis, growth, immune response, ion transport etc. biological progresses. In addition, the RT-PCR results of 3 differentially expressed genes were consistent with that of gene chip.【Conclusion】Combined with the related literatures, BMP7, MMP2, SNAI1, SFXN1, CDKNIC, MT3, POU1F1 etc. might have important effects on the formation of large flowers and small flowers and could be regarded as candidate genes, and offered the basic data for the further study on the molecule genetic mechanism of hair follicles’ formation in Hu sheep.

Key words: Hu sheep , hair follicles , microarray

SUN Wei, NI Rong, YIN Jin-Feng, DING Jia-Tong, ZHANG You-Fa, CHEN Ling, WU Wen-Zhong, ZHOU Hong. Screening Differentially Expressed Genes of Skin Tissue of Different Flowers Patterns of Hu Sheep[J].Scientia Agricultura Sinica, 2013, 46(2): 376-384.

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