Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (21): 4550-4557.doi: 10.3864/j.issn.0578-1752.2013.21.018

• HORTICULTURE • Previous Articles     Next Articles

Extensive Citrus Triploid Breeding by Crossing Monoembryonic Diploid Females with Allotetraploid Male Parents

 JIE  Kai-Dong-1, WANG  Hui-Qin-1, WANG  Xiao-Pei-1, LIANG  Wu-Jun-1, XIE  Zong-Zhou-1, YI  Hua-Lin-1, DENG  Xiu-Xin-1, Grosser  Jude W2, GUO  Wen-Wu-1   

  1. 1.College of Horticulture & Forestry Sciences, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070, China
    2.Citrus Research and Education Center, IFAS, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA
  • Received:2013-04-28 Online:2013-11-01 Published:2013-08-21

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Abstract: 【Objective】 The objective of this experiment is to produce citrus triploid hybrids by interploid crossing between elite monoembryonic diploid varieties as female parents and allotetraploid somatic hybrids. 【Method】 Pollinations were carried out between the selected male and female parents. Fruits were collected at 70-100 d after pollination and immature seeds were cultured in vitro. Ploidy level of the plantlets was determined by flow cytometry and chromosome counting. The genetic origin of triploids and tetraploids was analyzed by SSR markers.【Result】 In successive four years from 2009 to 2012, 14 ploidy crosses using eight diploid cultivars as seed parents and four allotetraploid somatic hybrids as pollen parents, were carried out with a total of 3 347 flowers pollinated. With 678 fruits harvested, an average fruit set ratio of 20.26% was obtained. As a result, 1 022 plants were recovered from 12 357 seeds cultured in vitro, which derived from 505 fruits. By flow cytometry analysis and chromosome counting, a total of 755 triploids and 19 tetraploids were verified. The results of SSR markers showed that all the triploids and tetraploids from the cross of Huanong red pummelo × NH were of hybrid origin. 【Conclusion】 The triploids obtained from these crosses are valuable materials for the selection of new seedless citrus varieties. The tetraploid hybrids are also of great value as potential parents for citrus triploid breeding.

Key words: citrus , triploids , sexual hybridization , ploidy analysis , SSR markers

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