Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (20): 4301-4309.doi: 10.3864/j.issn.0578-1752.2013.20.013

• HORTICULTURE • Previous Articles     Next Articles

Construction of the Suppression Subtractive Hybridization Library and Analysis of Related Genes of Spur-Type Apple (Malus domestica Borkh.)

 SONG  Yang-12, WU  Shu-Jing-1, ZHANG  Yan-Min-1, CHEN  Xue-Sen-1   

  1. 1.College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong
    2.Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning
  • Received:2012-12-13 Online:2013-10-15 Published:2013-07-25

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Abstract: 【Objective】The aim of this experiment is to explore genes related to spur type shoots of apple and to try to understand the mechanism of sport for breeding new spur type variety.【Method】A forward and reverse suppression subtractive hybridization library were constructed using the spur type shoots from sport and the standard shoots. Identification of the inserted cDNA fragments in subtracted library was done using real-time PCR.【Result】A total of 291 positive clones were obtained and the size of the inserts was 300 to 600 bp. By means of BLAST comparison of GenBank, 126 ESTs were found to share considerable homology with known genes while the rest 165 ESTs had no homology with known genes. The putative function of these genes was involved in metabolism, transcription, stress response, transport, photosynthesis, and signal transduction. The ESTs were significantly differently expressed between the spur type sport and the standard shoots. 【Conclusion】 Some genes related to shoot internode length of spur type apple were obtained through construction of the forward and reverse suppression subtractive hybridization library. The research results suggested that the genes play an important role during the shoots elongation of spur type apple.

Key words: apple , spur type , suppression subtractive hybridization(SSH) , expressed sequence tags , real-time PCR

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