Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4082-4090.doi: 10.3864/j.issn.0578-1752.2013.19.014

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Screening of Low Temperature Maize Stalk Decomposition Microorganism

 SA  Ru-La, GAO  Ju-Lin, YU  Xiao-Fang, HU  Shu-Ping   

  1. Agricultural College, Inner Mongolia Agriculture University, Hohhot 010019
  • Received:2013-04-28 Online:2013-10-01 Published:2013-07-16

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Abstract: 【Objective】 For the purpose of accelerating the northern region maize straw degradation speed, a research was conducted to screen microorganism which has a good ability to degradate maize straw under the conditions of low temperature. 【Method】 In this experiment, materials rich in cellulose were used as bacteria sources, through preliminary screening by filter-paper disintegration indicator, enzyme activity and straw degradation rate re-screening, the maize straw degradation strains were selected, then its composition was analyzed. 【Result】 Using this method, two cellulose-degrading microflora (designated as No.1 and No.8) capable of effectively degrading maize straw was enriched from plateau rotted sawdust successfully. The microflora No.1 and No.8 were inoculated in the medium with maize straw as a sole carbon and energy source. The degradation rate of maize straw were 30.21% and 32.21% after 15 days cultivation under 15℃. Two groups of composite microbial systems were composed of fungi and bacteria.No.1 strains of fungus were Trichoderma, and No.8 strains fungus were Penicillium. Two groups of composite microbial system was mainly composed of Clostridium bacteria, Bacillus and oxalic acid bacillus bacteria. 【Conclusion】 Screening of the strains under low temperature (15℃) could degrade the maize straw under laboratory conditions, and the microbial system was mainly comprised of Trichoderma, Penicillium, Clostridium, Bacillus and Herbaspirillum.

Key words: maize stalk , decomposition microorganism , low temperature , DGGE

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