Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4044-4057.doi: 10.3864/j.issn.0578-1752.2013.19.010

• PLANT PROTECTION • Previous Articles     Next Articles

Identification of Cercospora Species Associated with Maize Gray Leaf Spot in China

 LIU  Qing-Kui-1, QIN  Zi-Hui-1, ZHANG  Xiao-Li-1, JIANG  Kai-1, CHEN  Mao-Gong-1, WU  Xiao-Fei-1, HE  Yue-Qiu-2, WANG  Gui-Qing-3, JIN  Qi-Ming-4, WANG  Xiao-Ming-1   

  1. 1.Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081
    2.School of Agriculture and Biological Technic, Yunnan Agricultural University, Kunming 650201
    3.College of Agriculture, Liaocheng University, Liaocheng 252029, Shandong
    4.Plant Protection Institute of Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin
  • Received:2013-02-18 Online:2013-10-01 Published:2013-04-19

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Abstract: 【Objective】 The objective of this study is to understand the Cercospora species causing maize gray leaf spot (GLS) in China. 【Method】 The samples of GLS were widely collected from maize producing areas and strains were isolated using single-spore isolation technique. The causal agents were identified based on fungal morphology, culture and molecular characters. 【Result】One hundred and thirty-six strains were isolated from samples collected in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Shandong, Yunnan and Hubei provinces. Of them 65 strains were identified as C. zeae-maydis which found in northern provinces as well as in early years in Yunnan. Seventy-one were C. zeina only occurred in Yunnan and Hubei provinces. C. sorghi var. maydis, which was reported in China, was not detected in the strains. 【Conclusion】 In China, there are two Cercospora species causing GLS, which are C. zeae-maydis and C. zeina. The former is found in the northern China, including Heilongjiang, Jilin, Liaoning, Inner Mongolia and Shandong provinces, and in the strains collected in Yunnan before 2008, and the later was distributed in southwest China, such as Yunnan and Hubei provinces. C. zeina is firstly reported as a causal agent of gray leaf spot on maize in China.

Key words: gray leaf spot of maize , causal agent , identification , Cercospora

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