Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (16): 3305-3313.doi: 10.3864/j.issn.0578-1752.2013.16.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Study on the Genetic Basis of General Combining Ability with QTL Mapping Strategy

 HU  Wen-Ming, XU  Yang, ZHANG  恩Ying, XU  Chen-Wu   

  1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu
  • Received:2013-03-15 Online:2013-08-15 Published:2013-05-30

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Abstract: 【Objective】 General combining ability (GCA) is an important indicator for assessing parents. Studying the genetic basis of GCA and the feasibility of mapping QTL related with GCA (QTLGCA) may provide a foundation in breeding. 【Method】 A NCII mating design, where a set of recombinant inbred lines (RIL) derived from two pure lines was used as the tested lines and several randomly selected pure lines were used as testers, and QTL mapping strategy was applied to investigate the genetic component of GCA, the impact factors for QTLGCA mapping and the relationship between QTLGCA and QTL controlling trait per se. 【Result】 Results indicated that when a trait was controlled by one pair of alleles, the GCA effect estimate of RIL and QTLGCA mapping were all associated with additive effect, dominant effect of QTL controlling trait per se and the alleles frequency in testers. When a trait was controlled by two pairs of additive/dominance alleles, the GCA effect estimate of RIL and QTLGCA mapping did not associate with whether the linkage between genes or not, and the impact factors of GCA estimate and QTLGCA mapping were the same as that in one pair of alleles. When a trait was controlled by two pairs of interactive alleles, both GCA estimate and QTLGCA mapping were all associated with the alleles frequency in testers, the major effect of QTL controlling trait and interactive effect between QTL. In addition, GCA estimate was also influenced by the recombination rate between loci. 【Conclusion】 The allele frequency in testers and relative size of effects of QTL controlling trait per se were important factors not only to GCA estimation but also to QTLGCA mapping, and the difference between QTLGCA mapping and QTL mapping also depended on the genetic architecture of quantitative traits and analyzsis method of mapping QTL.

Key words: general combining ability , assessment of parents , QTL mapping , NCII mating design

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