植物基因组上游开放阅读框的挖掘方法及其翻译调控

doi:10.3864/j.issn.0578-1752.2013.16.002

Abstract

Abstract: Approximately 20% of plant genes have small upstream open reading frame (uORF), which are located in the 5' UTR of a mature mRNA. The mining methods, classification and evolution of the uORF homologous groups, and research progress of their translation regulation to downstream mORF were briefly introduced. Conserved peptide uORF (uORF) are ubiquitous in plants (Arabidopsis and rice) and insects (fruit fly) genomes, and they have similar diversity of sequences, but differ in their average length, genome clustering, and their preferential association with methyltransferases. Plants usually have shorter uORF than that of insects. Comparative analysis of the full-length cDNA sequences between Arabidopsis and rice showed that homology groups of uORF can play the regulatory functions on the downstream mORF, such as transcription factors, signal transduction factors, developmental signal molecules, and translation initiation factor eIF5. The Bayesian phylogenetic analysis of the uORF with the CHCH domain in the eukaryotic genomes showed that the homologous group 8 -like could be divided into five branches of taxa (green algae, arthropods, nematodes, invertebrates and fungi). uORF families could act as trans factors to regulate the expression of major ORF during the growth, development, and physiology in the eukaryotes. Quite large ratio of uORF can regulate the translation of mORF in response to the concentrations of polyamine, sucrose, phosphatidyl choline, and methyl transferase in plant genomes.

Key words: uORF , translational regulation , evolution , transcription factor , transcripts

WANG Ting-Zhang, HU Wang-Xiong, XU Jian-Hong, XUE Qing-Zhong. Mining Method of the Upstream Open Reading Frames and Their Translational Regulation in the Plant Genomes[J].Scientia Agricultura Sinica, 2013, 46(16): 3314-3323.

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Mining Method of the Upstream Open Reading Frames and Their Translational Regulation in the Plant Genomes

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