Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (15): 3285-3292.doi: 10.3864/j.issn.0578-1752.2013.15.024

• RESEARCH NOTES • Previous Articles     Next Articles

Screening of Microsatellite Markers in Bactrocera minax (Diptera: Tephritidae)

 GAO  Li-Zhi-1, LIU  Ying-Hong-1, WAN  Xuan-Wu-12, WANG  Jia-1, HONG  Feng-1   

  1. 1.Institute of Entomology, Southwest University, Chongqing 400716
    2.Sichuan Plant Protection Station, Chengdu 610041
  • Received:2013-01-28 Online:2013-08-01 Published:2013-04-23

PDF

545

Cited

2

Abstract: 【Objective】The objective of the study is to screen some highly polymorphic microsatellite markers for research on the population genetic structure of Chinese citrus fly (Bactrocera minax).【Method】Thirty-three pairs of microsatellite primers were selected from B. dorsalis, B. papayae, B. cucurbitae and B. cacuminata, which belonged to the same genus with B. minax. Then these primers were firstly screened through agarose gel electrophoresis of the PCR amplification product and rescreened through modified polyacrylamide gel electrophoresis. At last, the allele frequency, polymorphism information content, effective number of alleles and so on were used for analysis.【Result】There were only 12 pairs of microsatellite primers amid 33 pairs that could amplify the genomes of B. minax specifically, and the homologous rate was 36.4%. Twenty-three alleles were detected at the six microsatellite sites in B. minax, and the average number of alleles was 3.83. The average polymorphism information content of the six microsatellite primers was 0.2248, the highest was 0.4105, and the lowest was 0.0869. 【Conclusion】The screened 6 microsatellite primers of B. minax showed a good polymorphism, and could be used in the subsequent research.

Key words: Bactrocera minax , microsatellite primers , alleles , polymorphism

[1]汪兴鉴, 罗禄怡. 桔大实蝇的研究进展. 昆虫知识, 1995, 32(5): 310-315.

Wang X J, Luo L Y. The research advance in Bactrocera minax. Entomological Knowledge, 1995, 32(5): 310-315. (in Chinese)

[2]陈世骧, 谢蕴贞. 关于桔大实蝇学名及种征. 昆虫学报, 1955, 5(1): 123-126.

Chen S X, Xie Y Z. Taxonomic notes on the Chinese citrus fly Tetradacus citri (chen). Acta Entomologica Sinica, 1955, 5(1): 123-126. (in Chinese)

[3]Miyake T. Studies on the fruit-flies of Japan. The Bulletin of the Imperial Central Agricultural Experimental Station, 1919, 1(2): 85-160.

[4]Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 1996, 2: 225-238.

[5]Zane L, Bargelloni L, Patarnello T. Strategies for microsatellite isolation: a review. Molecular Ecology, 2002 11: 1-16.

[6]Kalia R K, Rai M K, Kalia S, Singh R, Dhawan A K. Microsatellite markers: an overview of the recent progress in plants. Euphytica, 2011, 177: 309-334.

[7]Shearman D C A, Gilchrist A S, Crisafullid D, Graham G, Lange C, Frommer M. Microsatellite markers for the pest fruit fly, Bactrocera papayae (Diptera: Tephritidae) and other Bactrocera species. Molecular Ecology Notes, 2006, 6: 4-7.

[8]Wu Y, Li Z, Wu J J. Polymorphic microsatellite markers in the melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). Molecular Ecology Resources, 2009, 9: 1404-1406

[9]Aketarawong N, Bonizzoni M, Malacrida A R, Gasperi G, Thanaphum S. Seventeen novel microsatellite markers from an enriched library of the pest species Bactrocera dorsalis sensu stricto. Molecular Ecology Notes, 2006, 6: 1138-1140.

[10]Dai S M, Lin C C, Chang C. Polymorphic microsatellite DNA markers from the oriental fruit fly Bactrocera dorsalis (Hendel). Molecular Ecology Notes, 2004, 4: 629-631.

[11]万宣伍, 刘映红, 张彬, 周浩东. 基于微卫星分子标记的重庆地区桔小实蝇遗传分化研究. 中国农业科学, 2010, 43(13): 2688-2696.

Wan X W, Liu Y H, Zhang B, Zhou H D. Genetic differentiation among populations of the oriental fruit fly Bactrocera dorsalis (Hendel) in Chongqing based on microsatellite markers. Scientia Agricultura Sinica, 2010, 43(13): 2688-2696. (in Chinese)

[12]Song S D, Ma J, Hughes J M. Polymorphic microsatellite DNA markers in Bactrocera cacuminata (Hering) (Diptera: Tephritidae). Molecular Ecology Notes, 2006, 6: 47-49.

[13]Kinnear M W, Bariana H S, Sved J A, Frommer M. Polymorphic microsatellite markers for population analysis of a tephritid pest species, Bactrocera tryoni. Molecular Ecology, 1998, 7: 1489-1495.

[14]Wan X W, Nardi F, Zhang B, Liu Y H. The oriental fruit fly, Bactrocera dorsalis, in China: origin and gradual inland range expansion associated with population growth. PLoS ONE, 2011, 6(10): e25238.

[15]Wan X W, Liu Y H, Zhang B. Invasion history of the oriental fruit fly, Bactrocera dorsalis, in the Pacific-Asia region: two main invasion routes. PLoS ONE, 2012, 7(5): e36176.

[16]张彬. 桔大实蝇线粒体全基因组分析及危险性实蝇类害虫检测基因芯片的开发[D]. 重庆: 西南大学, 2010: 55-113.

Zhang B. Analysis of complete mitochondrial genome of Bactrocera minax (Diptera: Tephritidae) and exploration of identification and detection for dangerous tephritid species by microarray[D]. Chongqing: Southwest University, 2010: 55-113. (in Chinese)

[17]Zhang B, Liu Y H, Wan X W, Wang Z L. Molecular phylogeny of Bactrocera species (Diptera: Tephritidae: Dacini) inferred from mitochondrial sequences of 16s rDNA and CO1 sequences. Florida Entomologist, 2010, 93(3): 369-377.

[18]张长禹. 五种实蝇的分子系统发育分析与分子快速鉴定研究[D]. 武汉: 华中农业大学, 2007: 33-39.

Zhang C Y. Molecular phylogenetic study and molecular identification in five species of fruit flies (Diptera:Tephritidae)[D]. Wuhan: Huazhong Agricultural University, 2007: 33-39. (in Chinese)

[19]耿金虎, 黄强, 徐希莲, 曾志将. 熊蜂ISSR-PCR 体系的建立与优化及其在亲缘关系分析中的应用. 昆虫学报, 2009, 52(2): 223-227.

Geng J H, Huang Q, Xu X L, Zeng Z J. Establishment and optimization of ISSR-PCR reaction system and its application in the genetic coefficient analysis of Bombus hypocrite Pérez (Hymenoptera: Apidae). Acta Entomologica Sinica, 2009, 52(2): 223-227. (in Chinese)

[20]Kojima T, Nagaoka T, Nada K, Ogihara Y. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theoretical and Applied Genetics, 1998, 96: 37-45.

[21]林杰君, 鲍毅新, 刘军, 张旭. ISSR分子标记及其在动物遗传结构研究中的应用. 生态学杂志, 2012, 31(5): 1319-1326.

Lin J J, Bao Y X, Liu J, Zhang X. ISSR marker and its applications in analyzing animal genetic structure: A review. Chinese Journal of Ecology, 2012, 31(5): 1319-1326. (in Chinese)

[22]周延清. DNA分子标记技术在植物研究中的应用. 北京: 化学工业出版社, 2005: 131-143.

Zhou Y Q. The Application of DNA Molecular Markers in Study on Plants. Beijing: Chemical Industry Press, 2005: 131-143. (in Chinese)

[23]彭文君, 罗其花, 李长春, 刘晓东, 马玉珍, 郭军, 安建东. 基于微卫星标记的东北黑蜂群体遗传多态性分析. 中国农业科学, 2012, 45(21): 4484-4491.

Peng W J, Luo Q H, Li C C, Liu X D, Ma Y Z, Guo J, An J D. Genetic diversity of northeastern black bee (Apis mellifera ssp.) in China based on microsatellite markers. Scientia Agricultura Sinica, 2012, 45(21): 4484-4491. (in Chinese)

[24]高帆, 张宗文, 吴斌. 中国苦荞SSR分子标记体系构建及其在遗传多样性分析中的应用. 中国农业科学, 2012, 45(6): 1042-1053.

Gao F, Zhang Z W, Wu B. Construction and application of SSR molecular markers system for genetic diversity analysis of Chinese tartary buckwheat germplasm resources. Scientia Agricultura Sinica, 2012, 45(6): 1042-1053. (in Chinese)

[25]Boststein D, White R L, Skolnick M, Davis R W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 1980, 32: 314-331.

[26]钟进城, 赵素军, 陈智华, 马志杰. 牦牛品种的遗传多样性及其分类研究. 中国农业科学, 2006, 39(2): 389-397.

Zhong J C, Zhao S J, Chen Z H, Ma Z J. Study on genetic diversity and classification of the Yak. Scientia Agricultura Sinica, 2006, 39(2): 389-397. (in Chinese)
[1] LIN Ping, WANG KaiLiang, YAO XiaoHua, REN HuaDong. Development of DNA Molecular ID in Camellia oleifera Germplasm Based on Transcriptome-Wide SNPs [J]. Scientia Agricultura Sinica, 2023, 56(2): 217-235.
[2] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[3] LIANG Peng,ZHANG TianWen,MENG Ke,SHAO ShunCheng,ZOU ShiFan,RONG Xuan,QIANG Hao,FENG DengZhen. Association Analysis of the ADIPOQ Variation with Sheep Growth Traits [J]. Scientia Agricultura Sinica, 2022, 55(11): 2239-2256.
[4] XU ZhiYing,WANG BaiCui,MA XiaoLan,JIA ZiMiao,YE XingGuo,LIN ZhiShan,HU HanQiao. Polymorphism Analysis Among Chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and Wheat 6A and 6D Based on Wheat SNP Chip [J]. Scientia Agricultura Sinica, 2021, 54(8): 1579-1589.
[5] ZHANG PengFei,SHI LiangYu,LIU JiaXin,LI Yang,WU ChengBin,WANG LiXian,ZHAO FuPing. Advance in Genome-Wide Scan of Runs of Homozygosity in Domestic Animals [J]. Scientia Agricultura Sinica, 2021, 54(24): 5316-5326.
[6] JunYi GAI,JianBo HE. Major Characteristics, Often-Raised Queries and Potential Usefulness of the Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis [J]. Scientia Agricultura Sinica, 2020, 53(9): 1699-1703.
[7] JianBo HE,FangDong LIU,WuBin WANG,GuangNan XING,RongZhan GUAN,JunYi GAI. Restricted Two-Stage Multi-Locus Genome-Wide Association Analysis and Its Applications to Genetic and Breeding Studies [J]. Scientia Agricultura Sinica, 2020, 53(9): 1704-1716.
[8] Yun PENG,TianGang LEI,XiuPing ZOU,JingYun ZHANG,QingWen ZHANG,JiaHuan YAO,YongRui HE,Qiang LI,ShanChun CHEN. Verification of SNPs Associated with Citrus Bacterial Canker Resistance and Induced Expression of SNP-Related Calcium-Dependent Protein Kinase Gene [J]. Scientia Agricultura Sinica, 2020, 53(9): 1820-1829.
[9] XU Yunbi,YANG QuanNü,ZHENG HongJian,XU YanFen,SANG ZhiQin,GUO ZiFeng,PENG Hai,ZHANG Cong,LAN HaoFa,WANG YunBo,WU KunSheng,TAO JiaJun,ZHANG JiaNan. Genotyping by Target Sequencing (GBTS) and Its Applications [J]. Scientia Agricultura Sinica, 2020, 53(15): 2983-3004.
[10] CHEN XiaoHong,HE JieLi,SHI TianTian,SHAO HuanHuan,WANG HaiGang,CHEN Ling,GAO ZhiJun,WANG RuiYun,QIAO ZhiJun. Developing SSR Markers of Proso Millet Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2020, 53(10): 1940-1949.
[11] WANG Jia,WANG Pan,FAN Huan,LIU YingHong. Comparison of Metabolic Profile Between Diapause-Destined and Non-Diapause-Destined Pupae of Bactrocera minax [J]. Scientia Agricultura Sinica, 2019, 52(6): 1021-1031.
[12] LIAN Yun,LI HaiChao,LI JinYing,WANG JinShe,WEI He,LEI ChenFang,WU YongKang,LU WeiGuo. Distribution of Soybean Cyst Nematode Resistance Allele Rhg1 and Rhg4 in Huang-Huai Soybean Varieties [J]. Scientia Agricultura Sinica, 2019, 52(15): 2559-2566.
[13] CAO XueTao, PEI ShengWei, ZHANG Jin, LI FaDi, LI Gang, LI WanHong, YUE XiangPeng. Screening of Y Chromosome Specific Primers and Y-SNPs in Sheep [J]. Scientia Agricultura Sinica, 2018, 51(15): 2990-2999.
[14] GAO XiaoLi, HU Jiang, GUO ShuZhen, SHI BinGang, XIE JianPeng, LUO YuZhu, WANG JiQing, MU YongJuan. Polymorphisms of DGAT1 Gene and Their Association with Milk Quality Traits in Yak [J]. Scientia Agricultura Sinica, 2017, 50(16): 3215-3225.
[15] LIU LiNa, YANG Jing, XU LiuYan, LI ChengYun. Genetic Diversity Analysis of Pi-ta Gene 3′-UTR in Rice Landraces [J]. Scientia Agricultura Sinica, 2017, 50(15): 2851-2860.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd