Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2584-2591.doi: 10.3864/j.issn.0578-1752.2013.12.020

• RESEARCH NOTES • Previous Articles     Next Articles

Cloning and Functional Study of ZmSCL7 in Zea mays

 GUO  Peng, XING  Xin, JIN  Hua, DONG  Yan   

  1. 1.College of Environment and Resources, Dalian Nationalities University, Dalian 116600, Liaoning
    2.Weihai Agricultural Bureau, Weihai 264200, Shandong
    3.Liaoning Forestry Vocational and Technical College, Shengyang 110101
  • Received:2012-12-30 Online:2013-06-15 Published:2013-03-21

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Abstract: 【Objective】The aim of this study is to clone SCARECROW-LIKE 7 (SCL7）gene, to analyze its molecular mechanisms and promote their applications in breeding. 【Method】 The total RNA from the leaves of tobacco was used as the template to design the degenerate primers based on homology cloning strategy, and then the full-length cDNA sequence of zmSCL7 was obtained through a combined reverse transcription-PCR (RT-PCR). Bioinformatics method was used to analyze the sequence characteristics of this gene. Northern blot was used to investigate the expression pattern. Western blot was used to investigate the transgenic analysis. Chlorophyll content, Fv/Fm, MDA and proline content were investigated for functional verification of salt resistance.【Result】The results indicated that cDNA of ZmSCL7 was 1 653 bp and contained a single open reading frame of 550 amino acid residues. Northern blot indicated that the mRNA accumulation of ZmSCL7 was induced by low temperatures, salt stress, abscisic acid (ABA) and H2O2. Additionally, compared with the control, transgenic ZmSCL7 tobacco, germination was inhibited less. Chlorophyll content, Fv/Fm, proline content increased and MDA content decreased. 【Conclusion】ZmSCL7, a transcription factor, was induced by a variety of stress and could increase salt tolerance by overexpression in tobacco.

Key words: Zea mays , SCARECROW-LIKE genes4（SCL7） , northern blot , western blot , functional study

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