Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (9): 1835-1845.doi: 10.3864/j.issn.0578-1752.2021.09.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Fusarium verticillioides Resistant Maize Inbred Line Development Using Host-Induced Gene Silencing Technology

HE KeWei1(),CHEN JiaFa2,ZHOU ZiJian2(),WU JianYu1,2()   

  1. 1College of Agronomy, Henan Agricultural University, Zhengzhou 450002
    2College of Life Sciences of Henan Agricultural University, Zhengzhou 450002
  • Received:2020-03-16 Accepted:2020-05-21 Online:2021-05-01 Published:2021-05-10
  • Contact: ZiJian ZHOU,JianYu WU E-mail:544220111@qq.com;zhouzijian19900601@163.com;wujianyu40@126.com

RichHTML

35

PDF

226

Abstract:

【Objective】Fusarium verticillioides (F. verticillioides) is a common pathogen, which can cause ear rot, stalk rot, seedling blight, and seed rot in maize. These diseases caused by Fusarium verticillioides not only affected the yield and quality of maize, but also seriously threatened to the safety of human and livestock by a variety of fungal toxins such as fumonisin which produced during the metabolic process of the pathogen. So far, there is no report about the major resistance gene cloned and utilized for Fusarium verticillioides in maize. Using host-induced gene silencing technology provides a new strategy for resistance breeding in maize. 【Method】Key genes associated with the Fusarium verticillioides development were cloned using homologous gene sequence method, and the dsRNA were produced by in-vitro transcription (IVT) assay. The dsRNA for different genes was premixed with suspension spores of Fusarium verticillioides used for RNA silencing experiment in vitro. For investigate the degree of disease, the seeds of the susceptible inbred line Xi502 were sterilized and inoculated, and then were cultured in a petri dish at 28℃ in the dark for 48 h. For investigate the incidence of the seeds after inoculation, glucose was added to the spore suspension mixed with dsRNA, then spore germination and mycelia growth were observed under the microscope after 25℃ culture for 24 h. The Xi502 seedlings of the trifoliate stage were transferred to the spore suspension with premixed dsRNA for culture, and the incidence of seedlings blight was observed after 7 days. In order to select the target gene for HIGS, combine the seed morphological observation result after inoculation and seedling inoculation result. Then, the silent vector about these key target genes were constructed and transferred into the susceptible inbred line Xi502. The transgenic seeds were evaluated by artificial inoculation. The total RNA of the transgenic seeds after inoculation was extracted, and the relative expression of target genes in F. verticillioide was analyzed by qRT-PCR to determine the silencing effect of HIGS line. 【Result】Eighteen candidate genes related to growth were cloned by homologous cloning method in Fusarium verticillioides. It was found that the disease level of seeds was significantly reduced after 11 candidate genes silencing by seed inoculation experiments. Furthermore, six of the 11 candidate target genes, deo, Ras2, Dpdc, Hsp90, Frp1, and Atg15, were found that response to the spore germination and mycelium growth after gene silencing. Finally, based on the results of seedling inoculation, 3 silencing target genes deo, Atg15 and Frp1 with significant inhibitory effect in vitro were selected. Then the silencing vector was constructed by combine three specific segments from the three target genes, transgenic plants were obtained. It was found that the resistance level was highly increased in T2-generation seeds compared to the none-transgenic plants. As well as the expression levels of all the three target genes were significantly decreased in Fusarium verticillioides. 【Conclusion】Three genes, deo, Atg15 and Frp1, are important for development of Fusarium verticillioides. By constructing transgenic HIGS plants for target gene deo, Atg15 and Frp1, the increase the resistance to Fusarium verticillioides in maize.

Key words: maize (Zea mays L.), Fusarium verticillioides, host-induced gene silencing, transgenic, ear rot

Table 1

List of candidate target genes"

基因 Gene 编号 ID 注释 Description 参考文献 Reference
rasG FVEG_09577 Ras GTP酶Ras GTPase putative expressed [30]
vea FVEG_09521 Velvet蛋白Velvet protein putative expressed [31]
mapk FVEG_05063 促分裂原活化蛋白Mitogen-activated protein kinase [32]
pka1 FVEG_05331 cAMP依赖蛋白激酶2 cAMP-dependent protein kinase type 2 [33]
CPP1 FVEG_09543 丝苏氨酸蛋白磷酸酶PP1-1 Serinethreonine-protein phosphatase PP1-1 [34]
gna2 FVEG_02792 鸟嘌呤核苷酸结合蛋白alpha-2亚基Guanine nucleotide-binding protein alpha-2 subunit [35]
aga1 FVEG_11573 丙氨酸乙醛酸盐氨基转移酶1 Alanine-glyoxylate aminotransferase 1 [36]
Atg15 FVEG_09194 自噬相关脂肪酶Autophagy-like lipase putative expressed [37]
cnb1 FVEG_07853 磷酸酶B亚基Calcineurin subunit B [38]
Frp1 FVEG_01458 F-box蛋白F-box protein putative expressed [39]
adeny FVEG_01363 腺苷酸环化酶Adenylate cyclase [33]
hsp90 FVEG_07470 热激蛋白90 Heat shock protein 90 [40]
Dpdc FVEG_07987 DNA聚合酶delta催化亚基DNA polymerase delta catalytic subunit [41]
gna3 FVEG_04170 鸟嘌呤核苷酸结合蛋白alpha-3亚基Guanine nucleotide-binding protein alpha-3 subunit [35]
cna1 FVEG_04738 丝苏氨酸蛋白磷酸酶2B催化亚基Serinethreonine-protein phosphatase 2B catalytic subunit [38]
ras2 FVEG_02837 Ras GTP酶Ras GTPase putative expressed [30]
deo FVEG_00771 脱氧辅蛋白合成酶Deoxyhypusine synthase [42]
gna1 FVEG_06962 鸟嘌呤核苷酸结合蛋白Guanine nucleotide-binding protein putative expressed [35]

Fig. 1

Preparation of candidate target gene silencing fragment dsRNA"

Fig. 2

The inhibition of seed rot after candidate target genes silencing in vitro *：P<0.05,**：P<0.01。The same as below"

Fig. 3

Effects of candidate target genes silencing on spore germination and mycelia growth of F. verticitlioides in vitro"

Fig. 4

The inhibition of seedling blight after candidate target genes silencing in vitro A: Appearance of maize seedlings at 7 days post inoculation. B: Statistics of the disease grade of seedling blight at 7 days post inoculation"

Fig. 5

Synthetic target sequence"

Fig. 6

F. verticitlioides resistance of HIGS transgenic maize A: Seeds appearance of WT and HIGS transgenic lines at 7 days post inoculation. B: Statistics of the disease grade of seed rot at 7 days post inoculation in WT and HIGS transgenic lines. C: The relative expression of 3 target genes at 3 days post inoculation in WT and HIGS transgenic lines"

[1] 王晓鸣, 晋齐鸣, 石洁, 王作英, 李晓. 玉米病害发生现状与推广品种抗性对未来病害发展的影响. 植物病理学报, 2006,36(1):1-11.
WANG X M, JIN Q M, SHI J, WANG Z Y, LI X. The status of maize diseases and the possible effect of variety resistance on disease occurrence in the future. Acta Phytopathologica Sinica, 2006,36(1):1-11. (in Chinese)
[2] KAMLE M, MAHATO D K, DEVI S, LEE K E, KANG S G, KUMAR P. Fumonisins: Impact on agriculture, food, and human health and their management strategies. Toxins, 2019,11(6):328.
[3] KNUTSEN H K, ALEXANDER J, BARREGÅRD L, BIGNAMI M, BRÜSCHWEILER B, CECCATELLI S, COTTRILL B, DINOVI M, EDLER L, GRASL-KRAUPP B, HOGSTRAND C, HOOGENBOOM L, NEBBIA C S, PETERSEN A, ROSE M, ROUDOT A C, SCHWERDTLE T, VLEMINCKX C, VOLLMER G, WALLACE C, DALL'ASTA G S, TARANU I, ALTIERI A, ROLDÁN- TORRES R, OSWALD I P. Risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. EFSA Journal, 2018,16(5):5242.
[4] ROSS P F, RICE L G, PLATTNER R D, OSWEILER G D, WILSON T M, OWENS D L, NELSON H A, RICHARD J L. Concentrations of fumonisin B1 in feeds associated with animal health problems. Mycopathologia, 1991,114(3):129-135.
[5] MARASAS W F O. Fumonisins: Their implications for human and animal health. Natural Toxins, 1995,3(4):193-198.
[6] GELDERBLOM W C A, JASKIEWICZ K, MARASAS W F O, THIEL P G, HORAK R M, VLEGGAAR R, KRIEK N P. Fumonisins: Novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Applied & Environmental Microbiology, 1988,54(7):1806-1811.
[7] YOSHIZAWA T, YAMASHITA A, LUO Y. Fumonisin occurrence in corn form high-risk and low-risk areas for human esophageal cancer in China. Applied and Environmental Microbiology, 1994,60(5):1626-1629.
[8] UENO Y, IIJIMA K, WANG S D, SUGIURA Y, SEKIJIMA M, TANAKA T, CHEN C, YU S Z. Fumonisins as a possible contributory risk factor for primary liver cancer: A 3-year study of corn harvested in Haimen, China, by HPLC and ELISA. Food and Chemical Toxicology, 1997,35(12):1143-1150.
[9] ZUO W, CHAO Q, ZHANG N, YE J, TAN G, LI B, XING Y, ZHANG B, LIU H, FENGLER K A, ZHAO J, ZHAO X, CHEN Y, LAI J, YAN J, XU M. A maize wall-associated kinase confers quantitative resistance to head smut. Nature Genetics, 2015,47(2):151-157.
[10] WANG C, YANG Q, WANG W, LI Y, GUO Y, ZHANG D, MA X, SONG W, ZHAO J, XU M. A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maize. The New Phytologist, 2017,215(4):1503-1515.
[11] LUNSFORD J N, FUTRELL M C, SCOTT G E. Maternal influence on response of corn to Fusarium moniliforme. Phytopathology, 1974,65:223-225.
[12] PÉREZ-BRITO S, JEFFERS D, GONZÀLEZ-DE-LEÓN D, KHAIRALLAH M, CORTÉS-CRUZ M, VELÀZQUEZ-CARDELAS G, AZPIROZ-RIVERO S, SRINIVASAN G. QTL mapping of Fusarium moniliforme ear rot resistance in highland maize, México. Agrociencia, 2001,35:181-196.
[13] ROBERTSON-HOYT L A, JINES M P, BALINT-KURTI P J, KLEINSCHMIDT C E, WHITE D G, PAYNE G A, MARAGOS C M, MOLNÁR T L, HOLLAND J B. QTL mapping for Fusarium ear rot and fumonisin contamination resistance in two maize populations. Crop Science, 2006,46(4):1734-1745.
[14] MASCHIETTO V, COLOMBI C, PIRONA R, PEA G, STROZZI F, MAROCCO A, ROSSINI L, LANUBILE A. QTL mapping and candidate genes for resistance to Fusarium ear rot and fumonisin contamination in maize. BMC Plant Biology, 2017,17(1):20.
[15] SEPTIANI P, LANUBILE A, STAGNATI L, BUSCONI M, NELISSEN H, MARIO ENRICO P, DELL’ACQUA M, MAROCCO A. Unravelling the genetic basis of Fusarium seedling rot resistance in the MAGIC maize population: Novel targets for breeding. Scientific Reports, 2019,9(1):5665.
[16] 张帆, 万雪琴, 潘光堂. 玉米抗穗粒腐病QTL定位. 作物学报, 2007,33(3):491-496.
ZHANG F, WAN X Q, PAN G T. Molecular mapping of QTL for resistance to maize ear rot caused by Fusarium moniliforme. Acta Agronomica Sinica, 2007,33(3):491-496. (in Chinese)
[17] DING J Q, WANG X M, CHANDER S, YAN J B, LI J S. QTL mapping of resistance to Fusarium ear rot using a RIL population in maize. Molecular Breeding, 2008,22(3):395-403.
[18] STAGNATI L, LANUBILE A, SAMAYOA L F, BRAGALANTI M, GIORNI P, BUSCONI M, HOLLAND J B, MAROCCO A. A genome wide association study reveals markers and genes associated with resistance to Fusarium verticillioides infection of seedlings in a maize diversity panel. Genes, Genomes, Genetics, 2019,9(2):571-579.
[19] ZILA C T, OGUT F, ROMAY M C, GARDNER C A, BUCKLER E S, HOLLAND J B. Genome-wide association study of Fusarium ear rot disease in the U.S.A. maize inbred line collection. BMC Plant Biology, 2014,14:372.
[20] LANUBILE A, FERRARINI A, MASCHIETTO V, DELLEDONNE M, MAROCCO A, BELLIN D. Functional genomic analysis of constitutive and inducible defense responses to Fusarium verticillioides infection in maize genotypes with contrasting ear rot resistance. BMC Genomics, 2014,15(1):710.
[21] YAO L, LI Y, MA C, TONG L, DU F, XU M. Combined genome-wide association study and transcriptome analysis reveal candidate genes for resistance to Fusarium ear rot in maize. Journal of Integrative Plant Biology, 2020,62(10):1535-1551.
[22] SUDARSHANA M R, ROY G, FALK B W. Methods for engineering resistance to plant viruses. Methods in Molecular Biology, 2007,354:183-195.
[23] WATERHOUSE P M, FUSARO A F. Viruses face a double defense by plant small RNAs. Science, 2006,313(5783):54-55.
[24] BAUM J A, BOGAERT T, CLINTON W, HECK G R, FELDMANN P, ILAGAN O, JOHNSON S, PLAETINCK G, MUNYIKWA T, PLEAU M, VAUGHN T, ROBERTS J. Control of coleopteran insect pests through RNA interference. Nature Biotechnology, 2007,25(11):1322-1326.
[25] KHATRI M, RAJAM M V. Targeting polyamines of Aspergillus nidulansby siRNA specific to fungal ornithine decarboxylase gene. Medical Mycology, 2007,45(3):211-220.
[26] TINOCO M, BÁRBARA D, DALL'ASTTA R, JOÃO P, ARAGÃO F. In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA. BMC Biology, 2010,8:27.
[27] NOWARA D, GAY A, LACOMME C, SHAW J, RIDOUT C, DOUCHKOV D, HENSEL G, KUMLEHN J, SCHWEIZER P. HIGS: Host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis. The Plant Cell, 2010,22(9):3130-3141.
[28] KOCH A, KUMAR N, WEBER L, KELLER H, IMANI J, KOGEL K H. Host-induced gene silencing of cytochrome P450 lanosterol C14 -demethylase-encoding genes confers strong resistance to Fusarium species. Proceedings of the National Academy of Sciences of the USA, 2013,110(48):19324-19329.
[29] THAKARE D, ZHANG J, WING R A, COTTY P J, SCHMIDT M A. Aflatoxin-free transgenic maize using host-induced gene silencing. Science Advances, 2017,3(3):e1602382.
[30] BLUHM B H, ZHAO X, FLAHERTY J E, XU J R, DUNKLE L D. RAS2 regulates growth and pathogenesis in Fusarium graminearum. Molecular Plant-Microbe Interactions, 2007,20(6):627-636.
[31] JIANG J, LIU X, YIN Y, MA Z. Involvement of a velvet protein FgVea in the regulation of asexual development, lipid and secondary metabolisms and virulence in Fusarium graminearum. PLoS ONE, 2011,6(11):e28291.
[32] ADÁM A L, KOHUT G, HORNOK L. Fphog1, a hog-type map kinase gene, is involved in multistress response in Fusarium proliferatum. Journal of Basic Microbiology, 2010,48(3):151-159.
[33] COLABARDINI A C, BROWN N A, SAVOLDI M, GOLDMAN M H S, GOLDMAN G H. Functional characterization of Aspergillus nidulans ypka, a homologue of the mammalian kinase SGK. PLoS ONE, 2013,8(3):e57630.
[34] CHOI Y E, SHIM W B. Functional characterization of Fusarium verticillioides CPP1, a gene encoding a putative protein phosphatase 2A catalytic subunit. Microbiology, 2008,154(1):326-336.
[35] EATON C J, CABRERA I E, SERVIN J A, WRIGHT S J, COX M P, BORKOVICH K A. The guanine nucleotide exchange factor RIC8 regulates conidial germination through Gα proteins in Neurospora crassa. PLoS ONE, 2012,7(10):e48026.
[36] VIJAI B, SABINE B, ALBERT V, GOPALAN S, WEI Y D. Alanine: glyoxylate aminotransferase 1 is required for mobilization and utilization of triglycerides during infection process of the rice blast pathogen, Magnaporthe oryzae. Plant Signaling & Behavior, 2012,7(9):1206-1208.
[37] NGUYEN L N, JÖRG B, LE G T, STÄRKEL C, SCHÄFER W. Autophagy-related lipase FgATG15 of Fusarium graminearum is important for lipid turnover and plant infection. Fungal Genetics and Biology, 2011,48(3):217-224.
[38] ZHANG H, GUO J, VOEGELE R T, ZHANG J, DUAN Y, LUO H, KANG Z. Functional characterization of calcineurin homologs pscna1/pscnb1 in Puccinia striiformis f sp tritici using a host-induced RNAi system. PLoS ONE, 2012,7(11):e49262.
[39] DUYVESTEIJN R G E, WIJK R V, BOER Y, REP M, HARING M A. Frp1 is a Fusarium oxysporum f-box protein required for pathogenicity on tomato. Molecular Microbiology, 2005,57(4):1051-1063.
[40] LAMOTH F, JUVVADI P R, FORTWENDEL J R, STEINBACH W J. Heat shock protein 90 is required for conidiation and cell wall integrity in Aspergillus fumigatus. Eukaryotic Cell, 2012,11(11):1324-1332.
[41] ZHAO P B, REN A Z, XU H J, LI D C. The gene fpk1, encoding a camp-dependent protein kinase catalytic subunit homolog, is required for hyphal growth, spore germination, and plant infection in Fusarium verticillioides. Journal of Microbiology & Biotechnology, 2010,20(1):208.
[42] WORIEDH M, HAUBER I, MARTINEZ-ROCHA A L, VOIGT C, MAIER F J, SCHRÖDER M, MEIER C, HAUBER J, SCHÄFER W. Preventing fusarium head blight of wheat and cob rot of maize by inhibition of fungal deoxyhypusine synthase. Molecular Plant-Microbe Interactions, 2011,24(5):619-627.
[43] CHEN J, DING J, LI H, LI Z, SUN X, LI J, WANG R, DAI X, DONG H, SONG W, CHEN W, XIA Z, WU J. Detection and verification of quantitative trait loci for resistance to Fusarium ear rot in maize. Molecular Breeding, 2012,30(4):1649-1656.
[44] MU C, GAO J, ZHOU Z, WANG Z, SUN X, ZHANG X, DONG H, HAN Y, LI X, WU Y, SONG Y, MA P, DONG C, CHEN J, WU J. Genetic analysis of cob resistance to F. verticillioides: Another step towards the protection of maize from ear rot. Theoretical and Applied Genetics, 2019,132(4):1049-1059.
[45] DOLORES B R, FERNANDO C G, CARLOS L G A, VICTOR H A R, JOSE L C S. Responses of maize landrace seedlings to inoculations of Fusarium spp. Open Access Library Journal, 2017,4(6):1-14.
[46] JU M, ZHOU Z, MU C, ZHANG X, GAO J, LIANG Y, CHEN J, WU Y, LI X, WANG S, WEN J, YANG L, WU J. Dissecting the genetic architecture of Fusarium verticillioides seed rot resistance in maize by combining QTL mapping and genome-wide association analysis. Scientific Reports, 2017,7:46446.
[1] CHAI HaiYan,JIA Jiao,BAI Xue,MENG LingMin,ZHANG Wei,JIN Rong,WU HongBin,SU QianFu. Identification of Pathogenic Fusarium spp. Causing Maize Ear Rot and Susceptibility of Some Strains to Fungicides in Jilin Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 64-78.
[2] MA XueMeng,YU ChengMin,SAI XiaoLing,LIU Zhen,SANG HaiYang,CUI BaiMing. PSORA: A Strategy Based on High-Throughput Sequence for Analysis of T-DNA Insertion Sites [J]. Scientia Agricultura Sinica, 2022, 55(15): 2875-2882.
[3] XIAO Fang,LI Jun,WANG HaoQian,ZHAI ShanShan,CHEN ZiYan,GAO HongFei,LI YunJing,WU Gang,ZHANG XiuJie,WU YuHua. Establishment and Application of A Duplex ddPCR Method to Quantify the NK603/zSSIIb Copy Number Ratio in Transgenic Maize NK603 [J]. Scientia Agricultura Sinica, 2021, 54(22): 4728-4739.
[4] JIN Rong,LIU Ming,ZHAO Peng,ZHANG QiangQiang,ZHANG AiJun,TANG ZhongHou. IbMKP6, A Mitogen-Activated Protein Kinase, Confers Low Temperature Tolerance in Sweetpotato [J]. Scientia Agricultura Sinica, 2021, 54(20): 4265-4273.
[5] SONG ShaoZheng,YU KangYing,ZHANG Ting,LU Rui,PAN ShengQiang,CHENG Yong,ZHOU MingMing. Preparation and Expression of rhPA/GH Double Transgenic Rabbits [J]. Scientia Agricultura Sinica, 2021, 54(2): 412-421.
[6] LI QinCheng,SHI Jie,HE KangLai,WANG ZhenYing. Effects of Chemical Control of Ear Borers on Reducing Fusarium verticillioides Ear Rot and Fumonisin Level [J]. Scientia Agricultura Sinica, 2021, 54(17): 3702-3711.
[7] ZHANG XiaoXue,SUN TianGe,ZHANG YingChun,CHEN LiHua,ZHANG XinYu,LI YanJun,SUN Jie. Identification of Xylosidase Genes from Verticillium dahliae and Functional Analysis Based on HIGS Technology [J]. Scientia Agricultura Sinica, 2021, 54(15): 3219-3231.
[8] ZHAO ZiQi,ZHAO YaQi,LIN ChangPeng,ZHAO YongZe,YU YuXiao,MENG QingLi,ZENG GuangYing,XUE JiQuan,YANG Qin. Precise Evaluation of 48 Maize Inbred Lines to Major Diseases [J]. Scientia Agricultura Sinica, 2021, 54(12): 2510-2522.
[9] HuiLin YU,Fang JIA,ZongHua QUAN,HaiLan CUI,XiangJu LI. Effects of Glyphosate on Weed Control, Soybean Safety and Weed Occurrence in Transgenic Herbicide-Resistant Soybean [J]. Scientia Agricultura Sinica, 2020, 53(6): 1166-1177.
[10] YaRu CHAI,YiJuan DING,SiYu ZHOU,WenJing YANG,BaoQin YAN,JunHu YUAN,Wei QIAN. Identification of the Resistance to Sclerotinia Stem Rot in HIGS-SsCCS Transgenic Arabidopsis thaliana [J]. Scientia Agricultura Sinica, 2020, 53(4): 761-770.
[11] WANG BaoBao,GUO Cheng,SUN SuLi,XIA YuSheng,ZHU ZhenDong,DUAN CanXing. The Genetic Diversity, Pathogenicity, and Toxigenic Chemotypes of Fusarium graminearum Species Complex Causing Maize Ear Rot [J]. Scientia Agricultura Sinica, 2020, 53(23): 4777-4790.
[12] LI YongXiang,LI ChunHui,YANG JunPin,YANG Hua,CHENG WeiDong,WANG LiMing,LI FengYan,LI HuiYong,WANG YanBo,LI ShuHua,HU GuangHui,LIU Cheng,LI Yu,WANG TianYu. Genetic Dissection of Heterosis for Huangzaosi, a Foundation Parental Inbred Line of Maize in China [J]. Scientia Agricultura Sinica, 2020, 53(20): 4113-4126.
[13] WEN Jing,GUO Yong,QIU LiJuan. Establishment and Application of Multiple PCR Detection System for Glyphosate-Tolerant Gene EPSPS/GAT in Soybean [J]. Scientia Agricultura Sinica, 2020, 53(20): 4127-4136.
[14] GONG Qiang,WANG Ke,YE XingGuo,DU LiPu,XU YanHao. Generation of Marker-Free Transgenic Barley Plants by Agrobacterium-Mediated Transformation [J]. Scientia Agricultura Sinica, 2020, 53(18): 3638-3649.
[15] LIU AiLi,WEI MengYuan,LI DongHua,ZHOU Rong,ZHANG XiuRong,YOU Jun. Cloning and Function Analysis of Sesame Galactinol Synthase Gene SiGolS6 in Arabidopsis [J]. Scientia Agricultura Sinica, 2020, 53(17): 3432-3442.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd