Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2514-2525.doi: 10.3864/j.issn.0578-1752.2013.12.012

• HORTICULTURE • Previous Articles     Next Articles

Expression and Genotype Analysis of VvmybA1 in Chinese Wild Grapes and Interspecific Hybrids

 JIAO  Jian, LIU  Chong-Huai, FAN  Xiu-Cai, ZHANG  Ying, SUN  Hai-Sheng, JIANG  Jian-Fu, LI  Min   

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009
  • Received:2012-12-11 Online:2013-06-15 Published:2013-02-19

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Abstract: 【Objective】The aim of this study is to detect the genotypes of Chinese wild grapes and the hybrids of V. amurensis and V. vinifera and compare the VvmybA1 gene sequences in different wild grapes, reveal the molecular mechanism of no anthocyanin collected in white-fruited Xiongyuebaiputao by analysising the expression and genotype of VvmybA1 in the interspecific hybrids (V. amurensis ×V. vinifera).【Method】The specific primers were designed to detect the genotype of different wild grapes by the means of molecular cloning and absolute quantitative real-time PCR. Using Real-time PCR methods, analysis the expression of VvmybA1 and UFGT in the the hybrids of V. amurensis and V. vinifera during veraison. 【Result】①There was no VvmybA1a allele detected in Chinese wild grapes. ②Comparing sequences of VvmybA1 gene in the different species of Chinese wild grapes with thoses of V. vinifera, extensive base deletion, insertion and substitution exist in promotor region, intron region and the third coding exon, and exhibits rich genetic diversity between species of Chinese wild grapes. Furthermore，there are several unique bases or mutations in the VvmybA1 gene of wild grapes which could be selected to be the molecule marker of distinguishing different species and cultivars. ③VvmybA1a allele has been discovered in Suxiaohong and the hybrids of V. amurensis × V. vinifera, including Gongniang 1, Beihong and Xiongyuebaiputao. In addition, Xiongyuebaiputao is the homozygous genotype of VvmybA1a. ④ RT-PCR analysis showed high expression of VvmybA1 and UFGT in Gongniang 1 during veraison, but they did not express in Xiongyuebaiputao during the entire mature period.【Conclusion】The result proved that VvmybA1a allele didn’t exist in Chinese wild grapes but existed in V. vinifera and its interspecific hybrids uniquely. High consanguinity was speculated between Suxiaohong and V. vinifera. Different VvmybA1 gene sequences of species in Vitis were obtained and compared with each other. The reason why Xiongyuebaiputao has no anthocyanin collected is that the homozygous genotype of VvmybA1a lead to the no expression of VvmybA1.

Key words: Chinese wild grapes, VvmybA1, anthocyanins, genotyping, skin color

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