Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 2076-2084.doi: 10.3864/j.issn.0578-1752.2013.10.013

• HORTICULTURE • Previous Articles     Next Articles

Effects of Exogenous Nitric Oxide and Abscisic Acid on Photosynthesis Characteristics in Pepper Seedlings Under Autotoxicity Stress

 ZHANG  Guo-Bin, YU  Ji-Hua, FENG  Zhi, MA  Yan-Xia, 吕Jian   

  1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070
  • Received:2012-11-20 Online:2013-05-15 Published:2013-03-29

PDF

734

Cited

6

Abstract: 【Objective】 The aim of the experiment was to explore the effects of exogenous NO donor (SNP) and abscisic acid on pepper resistance ability of autotoxicity. 【Method】 Pepper cultivar ‘Longjiao 2’ was used as materials in this experiment to investigate the effects of SNP (150 μmol•L-1) and ABA(100 μmol•L-1) on gas exchange parameters, cholorophyll fluorescence parameters and chlorophyll content in pepper seedlings under autotoxicity stress. The seedlings of pepper were treated with soil and substrates extracts of 3 years continuous cropping pepper. 【Result】 It was found that all of the soil and substrates extracts reduced Pn, Gs, Tr, Fv/Fm, ΦPSⅡ, qP, chlorophyll content and ratio of chlorophyll a/b obviously, but increased Ci and NPQ significantly. Under autotoxicity stress, exogenous NO treatments significantly increased Pn, Gs, Fv/Fm, ΦPSⅡ, qP and chlorophyll content, but reduced Ci and NPQ significantly, and had no obvious influence on Tr. Exogenous ABA treatments significantly increased Pn, Fv/Fm, ΦPSⅡ, qP and chlorophyll content, but reduced Ci and NPQ significantly, and had no obvious influence on Gs and Tr under autotoxicity stress.【Conclusion】The reduction in net photosynthesis rate caused by autotoxicity stress was considered to be a result of non-stomatal restriction mainly, and autotoxicity on pepper treated with soil extracts was serious greatly than substrates extracts. Under autotoxicity stress, exogenous NO and ABA application increased chlorophyll content, maintained PSII center activities and photosynthetic capacity, and enhanced autoxicity tolerance in pepper.

Key words: pepper , nitric oxide , abscisic acid , autotoxicity , gas exchange parameters , cholorophyll fluorescence parameters

[1]雷娟利, 周艳虹, 丁桔, 王礼, 喻景权. 不同蔬菜连作对土壤细菌 DNA分子水平多态性影响的研究. 中国农业科学,  2005, 38(10): 2076-2083.

Lei J L, Zhou Y H, Ding J, Wang L, Yu J Q. Effect of continuous cropping of different vegetables on DNA polymorphorism of soil bacterial. Scientia Agricultura Sinica，2005, 38(10): 2076-2083. (in Chinese)

[2]李刚, 文景芝, 吴凤芝, 张齐凤, 叶楠. 连作条件下设施黄瓜根际微生物种群结构及数量消长. 东北农业大学学报, 2006, 37(4): 444-448.

Li G, Wen J Zi, Wu F Z, Zhang Q F, Ye N. Microbial population structures and variation of microbial communities in rhizosphere of cucumber potted in the soil obtained from continuous cropping greenhouse. Journal of Northeast Agricultural University, 2006, 37(4): 444-448. (in Chinese)

[3]杨美森, 王雅芳, 干秀霞, 罗宏海, 张亚黎, 张旺锋. 外源一氧化氮对冷害胁迫下棉花幼苗生长、抗氧化系统和光合特性的影响. 中国农业科学, 2012, 45(15): 3058-3067.

Yang M S, Wang Y F, Gan X X, Luo H H, Zhang Y L, Zhang W F. Effects of exogenous nitric oxide on growth, antioxidant system and photosynthetic characteristics in seedling of cotton cultivar under chilling injury stress. Scientia Agricultura Sinica, 2012, 45(15): 3058-3067. (in Chinese)

[4]任旭琴, 刘美琴, 陈葛亮, 王蓉. 外源ABA对辣椒抗冷性生理指标的影响. 长江蔬菜, 2009(8): 30-32.

Ren X Q, Liu M Q, Chen G L, Wang R. Effect of exogenous ABA on chilling resistance in pepper. Journal of Changjiang Vegetables, 2009(8): 30-32. (in Chinese)

[5]曹慧, 王孝威, 邹岩梅, 束怀瑞. 外源NO对水分胁迫下平邑甜茶幼苗中几种氮代谢酶的影响. 园艺学报, 2009, 36 (6): 781-786.

Cao H, Wang X W, Zou Y M, Shu H R. Effects of exogenous nitric oxide on the several enzymes of nitrogen metabolism in Malus hupehensis(Pamp.) Rehd. seedlings under water stress. Acta Horticulturae Sinica, 2009, 36 (6): 781-786. (in Chinese)

[6]樊怀福, 郭世荣, 焦彦生, 张润花, 李娟. 外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响. 生态学报, 2007, 27 (2): 546-553.

Fan H F, Guo S R, JiaoY S, Zhang R H, Li J. The effects of exogenous nitric oxide on growth, active oxygenmetabolism and photosynthetic characteristics in cucumber seedlings under NaCl stress. Acta Ecologica Sinica, 2007, 27 (2): 546 -553. ( in Chinese)

[7]毛桂莲, 许兴, 谢亚军. 外源ABA对NaCl胁迫下枸杞幼苗生长和叶绿素荧光特性的影响.江苏农业科学, 2005(6): 111-114.

Mao G L, Xu X, Xie Y J. Effects of exogenous ABA on growth and chlorophyll fluorescence characteristics in wolfberry seedlings under NaCl stress. Jiangsu Agricultural Science, 2005(6): 111-114. ( in Chinese)

[8]耿广东, 张素勤, 程智慧. 辣椒根系分泌物的化感作用及其化感物质分析. 园艺学报, 2009, 36 (6): 873-878.

Geng G D, Zhang S Q, Cheng Z H. Allelopathy and allelochemicals of root exudates in hot pepper. Acta Horticulturae Sinica, 2009, 36 (6): 873-878.(in Chinese)

[9]李春龙, 贺阳冬, 陈华, 史伟, 练华山．辣椒连作障碍机制初探及其下茬作物的初选．安徽农业科学, 2007, 35 (26) : 8187-8188.

Li C L, He Y D, Chen H, Shi W, Lian H S. Elementary study on continuous cropping obstacle mechanism of red pepper and preliminary selection for its next- stubble crops. Journal of Anhui Agricultural Sciences, 2007, 35(26): 8187-8188. (in Chinese)

[10]侯永侠, 周宝利, 吴晓玲. 不同连作土壤对辣椒生长发育的影响研究. 北方园艺, 2009(8): 9-11.

Hou Y X, Zhou B L, Wu X L. The effect of the different soil of continuous crops time to pepper growth. Journal of Northern Horticulture, 2009(8): 9-11. (in Chinese)

[11]杨广君, 赵尊练, 巩振辉, 赵海燕, 梁丽鹏. 线辣椒根系分泌物对辣椒等受体作物的化感效应. 西北农林科技大学学报: 自然科学版, 2008, 36(10):146-157.

Yang G J, Zhao Z L, Gong Z H, Zhao H Y, Liang L P. Allelopathy functions of the root exudates ofline pepper to pepper and other crops. Journal of Northwest A&F University: National Science Edition, 2008, 36(10): 146-157. ( in Chinese)

[12]Qiao W H, Fan L M. Nitric oxide signaling in plant responses to abiotic stresses. Journal of Integrative Plant Biology, 2008, 50(10): 1238-1246.

[13]刘建新, 胡浩斌, 王鑫. 外源一氧化氮供体对镉胁迫下黑麦草幼苗活性氧代谢、光合作用和叶黄素循环的影响. 环境科学学报, 2009, 29(3): 626-633.

Liu J X, Hu H B, Wang X. Effects of an exogenous nitric oxide donor on active oxygenmetabolism, photosynthesis and the xanthophyll cycle in Ryegrass (Lolium perenne L.) seedlings under cadmium stress. Acta Scientiae Circumstantiae, 2009, 29(3): 626-633. (in Chinese)

[14]江力, 王东胜, 王能如, 张荣铣. 6-苄基腺嘌呤和脱落酸对不同发育阶段烟草叶片光合功能及过氧化氢水平的调控. 中国烟草学报, 2006,12(5): 38-42.

Jiang L, Wang D S, Wang N R, Zhang R X. Effects of 6-benzyladenine and abscisic acid on the photosynthetic functionand hydrogen peroxide content in tobacco leaves of different development phase. Acta Tabacaria Sincia, 2006,12(5):38-42. (in Chinese)

[15]Mata C G, Lamattina L. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiology, 2001, 126: 1196-1204.

[16]Uchida A, Jagendorf A T, Hibino T, Takabe T. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Science, 2002, 163: 515-523.

[17]Lu S Y, Su W, Li H H. Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2 and NO induced antioxidant enzyme activities. Plant Physiology and Biochemistry, 2009, (47): 132-138.

[18]高安妮, 田长平, 胡艳丽, 陈强, 毛志泉. 外源NO对连作条件下平邑甜茶幼苗生理特性的影响.中国农业科学 2011, 44(10): 2184-2192.

Gao A N, Tian C P, Hu Y L, Chen Q, Mao Z Q. Effects of exogenous nitric oxide on physiological characteristics of seedlings of Malus hupehensis Rehd. under continuous cropping. Scientia Agricultura Sinica, 2011, 44(10): 2184-2192. (in Chinese)

[19]张志刚, 尚庆茂. 低温、弱光及盐胁迫下辣椒叶片的光合特性. 中国农业科学, 2010, 43(1): 123-131.

Zhang Z G, Shang Q M. Photosynthetic characteristics of pepper leaves under low temperature, weak light and salt stress. Scientia Agricultura Sinica, 2010, 43(1): 123-131. (in Chinese)

[20]Yu J Q, Matsui Y. Effect of root exudates of cucumber and allelopathicals on the ion uptake by cucumber seedling. Journal of Chemical Ecology, 1996, 6: 817-834.

[21]Oleszek W, Jurzysta M. The allelopathic of alfalfa root medicagenic acid glycosides and their fate in soil environment.Ｐlant Soil, 1987, 98: 67-80.

[22]Fischer N H, Williamson G B, Weidenhamer J D, Richardson D R. In search of allelopathy in the Florida scrub: The role of terpenoids. Journal of Chemical Ecology, 1994, 20: 1355-1380.

[23]Li X G, Meng Q W, Jiang G Q, Zou Q. The susceptibility of cucumber and sweet pepper to chilling under low irradiance is related to energy dissipation and water-water cycle. Photosynthetica, 2003, 41(2): 259-265.

[24]许楠, 孙广玉. 低温锻炼后桑树幼苗光合作用和抗氧化酶对冷胁迫的响应. 应用生态学报, 2009, 20(4): 761-766.

Xu N, Sun G Y. Responses of mulberry seedling photosynthesis and antioxidant enzymes to chilling stress after low-temperature acclimation. Chinese Journal of Applied Ecology, 2009, 20(4): 761-766. (in Chinese)

[25]马向丽, 魏小红, 龙瑞军, 崔文娟, 万引琳. 外源一氧化氮提高一年生黑麦草抗冷性机制. 生态学报, 2005, 25(6): 1269-1274.

Ma X L, Wei X H, Long R J, Cui W J, Wan Y L. Studies on mechanism of enhancing the chilling resistance of annual ryegrass by exogenous nitric oxide. Acta Ecologica Sinica, 2005, 25(6): 1269-1274. (in Chinese)

[26]敬岩, 孙宝腾, 符建荣. 一氧化氮改善铁胁迫玉米光合组织结构及其活性. 植物营养与肥料学报, 2007, 13(5): 809-815.

Jing Y, Sun B T, Fu J R. Nitric oxide improves photosynthetic structure and activity in iron-deficient maize. Plant Nutrition and Fertilizer Science, 2007, 13(5): 809-815. (in Chinese)

[27]Farquhar G D, Sharky T D. Stomatal conductance and photosynthesis. Annual Review of Plant Physiology, 1982, 33: 317-345.

[28]马彦霞, 郁继华, 张国斌, 李雯琳, 曹刚. 谷胱甘肽对自毒作用下辣椒叶片光合特性的影响. 核农学报, 2012，26(2) : 396-402.

Ma Y X, Yu J H, Zhang G B, Li W L, Cao G. Effects of glutathione on photosynthetic characteristics of pepper leaves under autotoxicity. Journal of Nuclear Agricultural Sciences, 2012, 26(2): 396-402. (in Chinese)

[29]李亮亮, 李天来, 张恩平, 张文博, 衣宁宁. 自毒物质对番茄幼苗光合作用及保护酶活性的影响及碳化玉米芯的缓解作用. 华北农学报, 2010, 25 (1): 141-146.

Li L L, Li T L, Zhang E P, Zhang W B, Yi N N. Effect of on photosynthesis and activity of antioxidant enzyme of the tomato seeding under autotoxicity stress and alleviation by carbonized maize cob application. Acta Agriculturea Boreali Sinica, 2010, 25(1): 141-146. (in Chinese)

[30]周凯, 郭维明, 王智芳, 郝峰鸽. 菊花不同部位及根际土壤水浸液处理对光合作用的自毒作用研究. 中国生态农业学报, 2009, 17(2): 318-322.

Zhou K, Guo W M, Wang Z F, Hao F G. Autotoxicity of aquatic extracts from chrysanthemum and rhizosphere soil on photosynthesis in the same plant species. Chinese Journal of Eco-Agriculture, 2009, 17(2): 318-322. (in Chinese)

[31]Allen D J, Ort D R. Impact of chilling temperature on photosynthesis in warm climate plants. Trends Plant Science, 2001, 6: 36-42.

[32]Foyer C H, Noctor G. Oxygen processing in photosynthesis: a molecular approach. New Phytologist, 2000, 146: 359-388.

[33]Van K O, Snel J F H. The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynthesis Research, 1990, 25: 147-150.

[34]徐凯, 郭延平, 张上隆. 草莓叶片光合作用对强光的响应及其机理研究. 应用生态学报, 2005, 16(1): 73-78.

Xu K, Guo Y P, Zhang S L. Response of strawberry leaves photosynthesis to strong light and its mechanism. Chinese Journal of Applied Ecology, 2005, 16(1): 73-78. (in Chinese)

[35]Harbinson J, Genty B, Baker N R. The relationship between CO2 Assimilation and electron transport in leaves. Photosynthesis Research, 1990, 25: 213-224.

[36]汤日圣, 黄益洪, 唐现洪. 微生物源脱落酸 （ABA）对辣椒苗耐冷性的影响. 江苏农业学报，2008, 24(4): 467-470.

Tang R S, Huang Y H, Tang X H. Effect of microorganism-sourced ABA on chilling resistance of pepper seedling. Jiangsu Journal of Agricultural Sciences, 2008, 24(4): 467-470. (in Chinese)

[37]罗立津, 徐福乐, 翁华钦, 洪淑珠, 段留生, 李召虎. 脱落酸对甜椒幼苗抗寒性的诱导效应及其机理研究. 西北植物学报, 2011, 31(1): 94-100.

Luo L J, Xu F L, Weng H Q, Hong S Z, Duan L S, Li Z H. Inducing effects and its biological mechanisms of ABA on the chilling resistance of sweet pepper seedlings. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(1): 94-100. (in Chinese)

[38]李雪梅, 张利红, 何兴元, 王兰兰, 陈强. 脱落酸对UV-C胁迫下小麦幼苗光合特性及抗氧化酶活性的影响. 应用生态学报, 2006, 17(5): 822-826.

Li X M, Zhang L H, He X Y, Wang L L, Chen Q. Effects of abscisic acid on photosynthetic characteristics and antioxidant enzyme activities of wheat seedlings exposed to UV-C. Chinese Journal of Applied Ecology, 2006, 17(5): 822-826. (in Chinese)

[39]李雪梅, 陈强, 王兰兰, 郝林. 脱落酸对小麦幼苗光合及抗氧化酶的影响. 沈阳师范大学学报: 自然科学版, 2006, 24(2): 221-223.

Li X M, Chen Q, Wang L L, Hao L. Effects of abscisic acid on photosynthesis and antioxidant enzymes in wheat seedling. Journal of Shenyang Normal University: Natural Science, 2006, 24(2): 221-223. (in Chinese)

[40]赵可夫, 范海, Harris P J C. 盐胁迫下外源ABA对玉米幼苗耐盐性的影响. 植物学报, 1995, 37(4): 295-300.

Zhao K F, Fan H, Harris P J C. Effect of exogenous ABA on the salt tolerance of corn seedlings under salt stress. Acta Botanica Sinica, 1995, 37(4): 295-300. (in Chinese)

[41]Huang J Z, Chen Z Y. Effects of ABA，IAA and CaM on plasma membrane H+-ATPase activity of developing bean cotyledons. Acta Phytophysiologica Sinica, 1996, 22(4): 337-343.

[42]张韵, 郁继华, 钟新榕, 朱虹. 外源ABA和GA3对NaCl胁迫下黄瓜种子萌发特性的影响. 甘肃农业大学学报, 2006, 41(2): 27-30.

Zhang Y, Yu J H, Zhong X R, Zhu H. Effects of exogenous ABA and GA3 on germination characteristics of cucumber seeds under NaCl stress. Journal of Gansu Agricultural University, 2006, 41(2): 27-30. (in Chinese)

[43]吴锡冬, 李子芳, 张乃华, 李鹏民, 高辉远. 外源脱落酸对盐胁迫玉米激发能分配和渗透调节的影响. 农业环境科学学报, 2006, 25(2): 312-316.

Wu X D, Li Z F, Zhang N H, Li P M, Gao H Y. Effects of exogenous ABA on excitation energy distribution and osmotic adjustment of maize seedlings under salt stress. Journal of Agro-Environment Science, 2006, 25(2): 312-316. (in Chinese)

[44]王萍. 碳酸钠胁迫下羊草幼苗的生理效应及外源脱落酸的缓解效应. 草业学报, 1998,7(1): 24-28.

Wang P. Response of Leymus chinensis seedlings to sodium carbonate stress and mitigatory effect of external ABA. Acta Prataculturae Sinica, 1998, 7(1): 24-28. (in Chinese)
[1] DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.
[2] YuXia WEN,Jian ZHANG,Qin WANG,Jing WANG,YueHong PEI,ShaoRui TIAN,GuangJin FAN,XiaoZhou MA,XianChao SUN. Cloning, Expression and Anti-TMV Function Analysis of Nicotiana benthamiana NbMBF1c [J]. Scientia Agricultura Sinica, 2022, 55(18): 3543-3555.
[3] LIU RuiYao,HUANG GuoHong,LI HaiYan,LIANG MinMin,LU MingHui. Screening and Functional Analysis in Heat-Tolerance of the Upstream Transcription Factors of Pepper CaHsfA2 [J]. Scientia Agricultura Sinica, 2022, 55(16): 3200-3209.
[4] GONG XiaoYa,SHI JiBo,FANG Ling,FANG YaPeng,WU FengZhi. Effects of Flooding on Soil Chemical Properties and Microbial Community Composition on Farmland of Continuous Cropped Pepper [J]. Scientia Agricultura Sinica, 2022, 55(12): 2472-2484.
[5] ZHENG Wei,SHI Zheng,LONG Mei,LIAO YunCheng. Photosynthetic and Physiological Characteristics Analysis of Yellow- Green Leaf Mutant in Wheat of Jimai5265yg [J]. Scientia Agricultura Sinica, 2021, 54(21): 4539-4551.
[6] LI XueRu,SHI XiXiong,WANG JianZhong,ZHANG PanGao,TIAN Zhu,HAN Ling. Effect of Nitric Oxide Synthetase Inhibitor on Yak Meat Quality During Post-Mortem Aging [J]. Scientia Agricultura Sinica, 2020, 53(8): 1617-1626.
[7] SONG SongQuan,LIU Jun,XU HengHeng,LIU Xu,HUANG Hui. ABA Metabolism and Signaling and Their Molecular Mechanism Regulating Seed Dormancy and Germination [J]. Scientia Agricultura Sinica, 2020, 53(5): 857-873.
[8] ZHANG Xue,YANG HongKun,ZHENG Ting,XIAO Yun,MO Piao,FAN GaoQiong. Effects of Exogenous ABA on Pre-Harvest Sprouting Resistance and Quality of White and Red Wheat Cultivars [J]. Scientia Agricultura Sinica, 2020, 53(23): 4750-4763.
[9] LI Ying,ZHAO JiHao,LI JinRong,QIAN BiChang,LIU ZhaoXin,GAO Fang,YANG DongQing,LI XiangDong. Effects of Exogenous 6-BA on Root Growth and Pod Yield of Flooded Peanut at Different Growth Stages [J]. Scientia Agricultura Sinica, 2020, 53(18): 3665-3678.
[10] ZHANG BaoXi,WANG LiHao,ZHANG ZhengHai,CAO YaCong,YU Hailong. Development of New Varieties of ZHONGJIAO Pepper Resistant to Pepper Mild Mottle Virus Using L 3Gene [J]. Scientia Agricultura Sinica, 2020, 53(18): 3846-3855.
[11] CHANG GuoRong,LI RenJian,ZHANG Qi,ZHANG YuMing,HAN YuanHuai,ZHANG BaoJun. Identification of Co-Expression Genes Related to Endogenous Abscisic Acid in Response to the Stress of Sclerospora graminicola by WGCNA in Foxtail Millet [J]. Scientia Agricultura Sinica, 2020, 53(16): 3280-3293.
[12] YIN MeiQiang,WANG Dong,WANG JinRong,LAN Min,ZHAO Juan,DONG ShuQi,SONG Xi’E,ALAM Sher,YUAN XiangYang,WANG YuGuo,WEN YinYuan. Effects of Exogenous Nitric Oxide on Seed Germination and Starch Transformation of Sorghum Seeds Under Salt Stress [J]. Scientia Agricultura Sinica, 2019, 52(22): 4119-4128.
[13] TANG YaFei,PEI Fan,LI ZhengGang,SHE XiaoMan,YU Lin,LAN GuoBing,DENG MingGuang,HE ZiFu. Identification of Viruses Infecting Peppers in Guangdong by Small RNA Deep Sequencing [J]. Scientia Agricultura Sinica, 2019, 52(13): 2256-2267.
[14] XU GuangChun, GU ZhongYan, XU DeJin, XU XiaoLong, XU Lu. Calculation Methods for the Surface Free Energy of Pepper Leaf Surface [J]. Scientia Agricultura Sinica, 2018, 51(16): 3084-3094.
[15] YANG LiuYan, CHEN JingJing, CHEN NianLai. Responses of Leaf Assimilate Export To Lowlight Stress in Melon [J]. Scientia Agricultura Sinica, 2018, 51(13): 2561-2569.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd