Scientia Agricultura Sinica ›› 2020, Vol. 53 ›› Issue (18): 3846-3855.doi: 10.3864/j.issn.0578-1752.2020.18.018

• RESEARCH NOTES • Previous Articles    

Development of New Varieties of ZHONGJIAO Pepper Resistant to Pepper Mild Mottle Virus Using L 3Gene

ZHANG BaoXi(),WANG LiHao(),ZHANG ZhengHai,CAO YaCong,YU Hailong   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2020-02-14 Accepted:2020-04-15 Online:2020-09-16 Published:2020-09-25
  • Contact: LiHao WANG E-mail:zhangbaoxi@caas.cn;wanglihao@caas.cn

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Abstract:

Hot (sweet) pepper is the largest area vegetable crop in China, with an annual planting area of 2.2×106 hm 2, including 5×105 hm2 of sweet pepper. Beside traditional diseases, in recent years, pepper milt mottle virus (PMMoV) as a new popular disease broke out, which seriously restricted the production of sweet pepper. At the same time, consumers put forward higher requirements for the quality and diversity of varieties. Our research group has introduced more than 1 400 accessions of hot (sweet) pepper germplasm resources from all over the world. Through identification and evaluation, more than 120 accessions with good characteristics such as antiviral disease, powdery mildew, large fruit, thin skin, good glossiness and high quality have been screened out. The molecular markers closely linked with TMV (L3, L4), TSWV (Tsw) and other important characters were constructed. The accuracy of assisted breeding was over 90%. Through the combination of conventional breeding techniques and molecular markers, the core backbone parental sweet pepper inbred line ‘0516’ was created with good combining ability, resistant to TMV, ToMV, PMMoV (P0,1,2), CMV and Phytophthora capsici, large fruit, uniform and good glossiness, which contained L3 gene resistant to PMMoV (P0,1,2). Four new varieties Zhongjiao 105, 106, 107 and 108 with high quality, multi resistance and adaptability to different ecological areas were developed. These new varieties contained L3 gene, which was resistant to TMV, PMMoV (P0,1,2), CMV and disease; the shape, size, color, uniformity and other commodity quality, vitamin C and other nutritional quality of fruits were significantly improved. It was important to improve the disease resistance and quality of sweet pepper.

Key words: sweet pepper, capsicum, pepper milt mottle virus (PMMoV), breeding of new varieties

Table 1

Statistical table of germplasm resources of introduced sweet pepper and pepper in different years from 2001 to 2015"

引进地区
Introduction of the region		 年份 Year 合计
Total
2001—2005 2006—2010 2010—2015
中国各省、市、区Chinese provinces, cities and districts 230 322 336 888
欧美 Europe and America 101 156 181 438
日本Japan 12 2 1 15
南亚South Asia 1 10 8 19
其他Other 22 31 7 60
合计Total 367 521 533 1420

Table 2

Excellent breeding original materials were identified and evaluated from 2001 to 2015"

材料特性
Material property		 来源
Source		 份数
Number		 代表种质
Typical germplasm
抗TMV TMV-resistant 中国、法国、日本、美国等 China, France, Japan, America, etc 21 Alliance;斯玛特;考曼其
Alliance;Smart; Kaomanchi
抗CMV CMV-resistant 泰国、法国等 Thailand, France, etc 9 OrientE3 (MaxzxMax3) 51-39; OrientE3 (MaxzxMax3) 51-39b; OrientE3(Max1xNana)
抗疫病 Phytophthora-resistant 泰国、法国等 Thailand, France, etc 10 RP30; RP38; RP40; RP4
抗炭疽 Anthracnose-resistant 亚蔬中心 Asian Vegetable Research and Development Center 5 PBC932; PBC81
抗疮痂 Scab-resistant 中国、泰国、法国等 China, Thailand, France, etc 7 PS09979325 XCR10;PBC446
抗PVY PVY-resistant 中国、法国、美国等 China, France, America, etc 21 Alliance;科曼;斯特朗
Alliance;Keman; Sitelang
抗青枯 Bacterial wilt-resistant 中国等 China, etc 13 马来西亚椒;肉厚牛角椒;PBC204
Malaysian; Thick horn pepper; PBC204
抗CVMV CVMV-resistant 中国等 China, etc 12 GTCV1; GTCV2; GTCV3
抗蓟马 Thrip-resistant 荷兰等 Holland, etc 10 Bisbas; Ac 1979
抗线虫 Nematode-resistant 法国等 France, etc 5 DH330; DH149; CM334
耐低温、弱光
Tolerance to low temperature and weak light		 荷兰等 Holland, etc 7 37-74; 37-82等
耐寒 cold-resistant 美国 America 3 Florist type; Poinsettia
其他 Other 中国、俄罗斯、日本等 China, Russia, Japan, etc 9

Table 3

Molecular marker assisted breeding system for disease resistance of pepper"

抗病种类
Disease-resistant species		 基因名称
Gene name		 显/隐性
Dominant/
Recessive		 位座
Chromosomal location		 连锁标记
Linkage marker		 参考文献
Reference
烟草花叶病毒Tobacco mosaic virus L3,4 显性Dominant P11 SCAR [27-28]
番茄斑点萎蔫病毒Tomato spot wilt virus Tsw 显性Dominant P10 CAPS [29-30]
黄瓜花叶病毒Cucumber mosaic virus QTL (2) 共显性Codominant P12 KASP-UN54228 [31]
马铃薯Y病毒Potato virus Y Pvr4 显性Dominant P10 CAPS [32-33]
疮痂病
Bacterial spot		 Bs2 显性Dominant - SCAR-A2 [27,34]
Bs3 显性Dominant P2 SCAR [27,35]
疫病
Phytophthora capsici		 QTL (18) 共显性Codominant P5 KASP-5-160 专利技术,未发表
Patented technology and unpublished
炭疽病Anthracnose QTL (P5.1) 共显性Codominant P5 KASPUN27353 [18]
白粉病Powdery mildew QTL (5) 共显性Codominant P6 SSRH6-96 [36]
根结线虫
Root-knot nematode		 N 显性Dominant P9 SCAR [25]
Me1 显性Dominant P9 16830CAPS [37]

Fig. 1

The creation process of the sweet pepper inbred line 0516"

Fig. 2

Identification of molecular markers of L3 gene in the middle generation M: 100 bp marker; 1-24: 0516 intergenerational strain F3 different individual plants"

Table 5

The average equivalent yield per mu of incomplete double-row hybrid combinations ( IVF farm in 2010)"

组合
Combination		 亩产
Acre yield
(kg/667 m2)		 组合
Combination		 亩产
Acre yield
(kg/667 m2)
A×B 4257.68 B×F 4118.73
A×C 3741.87 C×D 3774.11
A×D 3551.78 C×E 4404.98
A×E 4518.93 C×F 4035.35
A×F 4353.29 D×E 4669.00
B×C 4302.15 D×F 4452.23
B×D 4624.53 E×F 3251.63
B×E 4741.81

Table 6

General combining ability performance of 6 materials yields"

材料 Material GCA
AF5 -101.828
0516 222.442
1421 -134.846
RQN 27.792
KLS 130.732
0601M -144.292

Fig. 3

Zhongjiao brand series sweet (chili) pepper varieties"

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