利用WGCNA鉴定谷子内源脱落酸响应禾生指梗霉胁迫的共表达基因

doi:10.3864/j.issn.0578-1752.2020.16.007

Abstract

Abstract:

【Objective】Abscisic acid (ABA), as a stress hormone, plays an important role in plant growth and development, biological and abiotic stresses. The ABA receptor protein PYR/PYL/PCAR and SNF1-related protein kinase (SnRK2) are important regulatory factors that mediate ABA signaling. The objective of this study is to predict the regulatory roles of ABA and the key genes in its signaling pathway in foxtail millet (Setaria italica) downy mildew caused by Sclerospora graminicola, and to provide a reference for the research of endogenous ABA in S. italica in response to the infection of S. graminicola.【Method】S. italica variety Jingu 21 infected by S. graminicola was used for transcriptome sequencing and ABA content measurement, and the PYL and SnRK2 family genes in the ABA signaling pathway were identified and analyzed based on the whole genome sequence of S. italica. A weighted gene co-expression network analysis (WGCNA) was constructed using the transcriptome, and it was associated with the content of host endogenous ABA in the context of S. graminicola infection to predict the key genes in the interaction between S. italica and S. graminicola regulated by ABA and the PYL and SnRK2 genes in its downstream signaling. The candidate genes were validated by qRT-PCR.【Result】There were 11 PYL and 11 SnRK2 family genes in S. italica, which were relatively conservative in the Gramineae, and ABA responsive elements were predicted in the promoters of PYL and SnRK2 family genes. The endogenous ABA in the host accumulated at the first and second stages after S. graminicola infection, and its content (22.50 and 18.08 ng·mL^-1, respectively) was significantly higher than that of the control group. However, the content of ABA decreased at the third, fourth, and fifth stages, which was lower than that of the control group. For the WGCNA, a total of 34 gene co-expression modules were constructed by using 18 535 genes. The MEpaleturquoise and MEbrown modules were predicted as the core candidate modules through the association analysis of ABA content and PYL, SnRK2 family genes. GO function enrichment and module key gene mining revealed that one PYL family gene (Seita.1G030500), two SnRK2 family genes (Seita.2G394500 and Seita.3G03200), and three core genes (Seita.4G105600, Seita.6G218100, and Seita.9G138400) might be involved in the interaction between S. italica and S. graminicola during the regulation of ABA and its signal transduction. After comparing three predicted core genes with the reference genes in Oryza sativa and Arabidopsis thaliana databases, Seita.4G105600 was identified as the transducin/WD40 repeat-like superfamily protein, Seita.6G218100 as the WRKY57 transcription factor, and Seita.9G138400 as the TIFY transcription factor. qRT-PCR analysis showed that Seita.2G394500, Seita.4G105600, and Seita.6G218100 genes were up-regulated at the early stage of S. graminicola infection.【Conclusion】The ABA accumulates in S. italica infected by S. graminicola. One PYL family gene, two SnRK2 family genes, two transcription factor genes, and one WD40 family protein gene were predicted as the key genes related to the response to S. graminicola infection. qRT-PCR results showed that one SnRK2 gene, one WD40 family protein gene, and one WRKY57 transcription factor gene may play an important role in the response of ABA in S. italica to the infection of S. graminicola.

Key words: foxtail millet (Setaria italica), abscisic acid, Sclerospora graminicola, PYL, SnRK2, weighted gene co-expression network analysis (WGCNA)

CHANG GuoRong,LI RenJian,ZHANG Qi,ZHANG YuMing,HAN YuanHuai,ZHANG BaoJun. Identification of Co-Expression Genes Related to Endogenous Abscisic Acid in Response to the Stress of Sclerospora graminicola by WGCNA in Foxtail Millet[J].Scientia Agricultura Sinica, 2020, 53(16): 3280-3293.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2020.16.007

https://www.chinaagrisci.com/EN/Y2020/V53/I16/3280

Figures/Tables 11

Table 1

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Table 2

GO enrichments of network modules"

类型 Type	基因编号 Gene ID	GO富集 GO term	P-value
MEpaleturquoise	GO:0000775	染色体、着丝粒区域Chromosome, centromeric region	0.02291
	GO:0008135	翻译因子活性、核酸结合位点Translation factor activity, nucleic acid binding	0.03575
	GO:0006075	1-3-beta-D-葡聚糖生物合成过程 1-3-beta-D-glucan biosynthetic process	0.0002
	GO:2000112	调控细胞大分子生物合成过程Regulation of cellular macromolecule biosynthetic process	0.0015
	GO:0051552	类黄酮代谢过程Flavone metabolic process	0.0249
	GO:0051553	类黄酮生物合成过程Flavone biosynthetic process	0.0249
	GO:0055074	钙离子平衡Calcium ion homeostasis	0.0249
	GO:0016780	磷酸转移酶活性,用于其他取代磷酸基Phosphotransferase activity, for other substituted phosphate groups	0.02825
MEbrown	GO:0003995	脂酰CoA脱氢酶活性Acyl-CoA dehydrogenase activity	0.001849608
	GO:0051171	调节氮化合物代谢过程Regulation of nitrogen compound metabolic process	1.95E-05
	GO:0009755	激素介导的信号通路Hormone-mediated signaling pathway	7.67E-05
	GO:0071383	细胞对类固醇激素刺激的反应Cellular response to steroid hormone stimulus	8.79E-04
	GO:0009725	激素响应Response to hormone	0.002160987
	GO:0009742	油菜素类内酯介导的信号通路Brassinosteroid mediated signaling pathway	0.002538882
	GO:0002376	免疫系统的过程Immune system process	0.003433683
	GO:0043207	对外界生物刺激的反应Response to external biotic stimulus	0.018615083
	GO:0009873	乙烯激活的信号通路Ethylene-activated signaling pathway	0.018737534
	GO:0009607	生物刺激反应Response to biotic stimulus	0.019137736
	GO:0080134	应激反应的调节Regulation of response to stress	0.019394085
	GO:0006631	脂肪酸代谢过程Fatty acid metabolic process	0.022668677
	GO:0009744	蔗糖响应Response to sucrose	0.023618658
	GO:0044038	细胞壁大分子生物合成过程Cell wall macromolecule biosynthetic process	0.033696381
	GO:0009620	响应真菌Response to fungus	0.043398934

Table 2

Fig. 6

Table 3

Fig. 7

Fig. 8

References 40

[1]	CUTLER S R, RODRIGUEZ P L, FINKELSTEIN R R, ABRAMS S R. Abscisic acid: Emergence of a core signaling network. Annual Review of Plant Biology, 2010,61:651-679. DOI: 10.1146/annurev- arplant-042809-112122. pmid: 20192755
[2]	丁冰杰, 孔祥强, 董合忠. 脱落酸受体PYLs的结构与功能研究进展. 分子植物育种, http://kns.cnki.net/kcms/detail/46.1068.s. 20191031.1538.004.html.. pmid: 31598026
	DING B J, KONG X Q, DONG H Z. Research progress on the structure and function of abscisic acid receptor PYLs. Molecular Plant Breeding, http://kns.cnki.net/kcms/detail/46.1068.s.20191031.1538. 004.html..(in Chinese) pmid: 31598026
[3]	NEMHAUSER J L, HONG F X, CHORY J. Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 2006,126:467-475. DOI: 10.1016/j.cell.2006.05.050. pmid: 16901781
[4]	张雪峰. 中国谷子产业发展问题研究[D]. 哈尔滨: 东北农业大学, 2013.
	ZHANG X F. Studies on the issues of millet industry development in China. Harbin: Northeast Agricultural University, 2013. (in Chinese)
[5]	JIANG C J, SHIMONO M, SUGANO S, KOJIMA M, YAZAWA K, YOSHIDA R, INOUE H, HAYASHI N, SAKAKIBARA H, TAKATSUJI H. Abscisic acid interacts antagonistically with salicylic acid signaling pathway in rice-Magnaporthe grisea interaction. Molecular Plant-Microbe Interactions, 2010,23(6):791-798. DOI: 10.1094/MPMI-23-6-0791. doi: 10.1094/MPMI-23-6-0791 pmid: 20459318
[6]	CAO J D, YANG C, LI L J, JIANG L, WU Y, WU C W, BU Q Y, XIA G X, LIU X Y, LUO Y M, LIU J. Rice plasma membrane proteomics reveals Magnaporthe oryzae promotes susceptibility by sequential activation of host hormone signaling pathways. Molecular Plant- Microbe Interactions, 2016,29(11):902-913. DOI: 10.1094/MPMI- 08-16-0165-R.
[7]	SONG W W, MA X R, TAN H, ZHOU J Y. Abscisic acid enhances resistance to Alternaria solani in tomato seedlings. Plant Physiology and Biochemistry, 2011,49:693-700. DOI: 10.1016/j.plaphy.2011. 03.018. doi: 10.1016/j.plaphy.2011.03.018 pmid: 21530290
[8]	MUKHERJEE A, MAZUMDER M, JANA J, SRIVASTAVA A K, MONDAL B, DE A, GHOSH S, SAHA U, BOSE R, CHATTERJEE S, DEY N, BASU D. Enhancement of ABA sensitivity through conditional expression of the ARF10 gene in Brassica juncea reveals fertile plants with tolerance against Alternaria brassicicola. Molecular Plant-Microbe Interactions, 2019,32(10):1429-1447. DOI: 10.1094/ MPMI-05-19-0132-R. doi: 10.1094/MPMI-05-19-0132-R pmid: 31184524
[9]	黎家, 李传友. 新中国成立70年来植物激素研究进展. 中国科学: 生命科学, 2019,49(10):1227-1281.
	LI J, LI C Y. Seventy-year major research progress in plant hormones by Chinese scholars. Scientia Sinica Vitae, 2019,49(10):1227-1281. (in Chinese)
[10]	FUJITA Y, NAKASHIMA K, YOSHIDA T, KATAGIRI T, KIDOKORO S, KANAMORI N, UMEZAWA T, FUJITA M, MARUYAMA K, ISHIYAMA K, et al. Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant and Cell Physiology, 2009,50(12):2123-2132. DOI: 10.1093/pcp/pcp147. pmid: 19880399
[11]	GEIDER D, SCHERZER S, MUMM P, STANGE A, MARTEN I, BAUER H, ACHE P, MATSCHI S, LIEAE A, AL-RASHEID K A S, ROMEIS T, HEDRICH R. Activity of guard cell anion channel SLAC1 is controlled by drought-stress signaling kinase-phosphatase pair. Proceedings of the National Academy of Sciences of the United State of America, 2009,106(50):21425-21430. DOI:10.1073/pnas. 0912021106.
[12]	YIN P, FAN H, HAO Q, YUAN X Q, WU D, PANG Y X, YAN C Y, LI W Q, WANG J W, YAN N. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nature Structural and Molecular Biology, 2009,16(12):1230-1236. DOI: 10.1038/nsmb. 1730. pmid: 19893533
[13]	HAO Q, YIN P, LI W Q, WANG L, YAN C Y, LIN Z H, WU J Z, WANG J W, YAN S F, YAN N. The molecular basis of ABA-independent inhibition of PP2Cs by a subclass of PYL proteins. Molecular Cell, 2011,42:662-672. DOI: 10.1016/j.molcel.2011.05. 011. pmid: 21658606
[14]	CAI Z Y, LIU J J, WANG H J, YANG C J, CHEN Y X, LI Y C, PAN S J, DONG R, TANG G L, BARAJAS-LOPEZ J, FUJII H, WANG X L. GSK3-like kinases positively modulate abscisic acid signaling through phosphorylating subgroup III SnRK2s in Arabidopsis. Proceedings of the National Academy of Sciences of the United State of America, 2014,111(26):9651-9656. DOI: 10.1073/pnas.1316717111.
[15]	易文凯, 王佳, 杨辉, 田云, 卢向阳. 植物ABA受体及其介导的信号转导通路. 植物学报, 2012,47(5):515-524.
	YI W K, WANG J, YANG H, TIAN Y, LU X Y. Abscisic acid receptors: Abscisic acid signaling transduction pathways in plants. Chinese Bulletin of Botany, 2012,47(5):515-524. (in Chinese)
[16]	ZHANG F, ZENG D, HUANG L Y, SHI Y Y, CHEN T J, ZHANG F, ZHOU Y L. Stress-activated protein kinase OsSAPK9 regulates tolerance to salt stress and resistance to bacterial blight in rice. Rice, 2019,12:80. DOI: 10.1186/s12284-019-0338-2. pmid: 31712918
[17]	YAO L S, LI Y M, MA C Y, TONG L X, DU F L, XU M L. Combined genome-wide association study and transcriptome analysis reveal candidate genes for resistance to Fusarium ear rot in maize. Journal of Integrative Plant Biology, 2020. DOI: 10.1111/jipb.12911. pmid: 32542992
[18]	FINN R D, CLEMENTS J, EDDY S R. HMMER web server: Interactive sequence similarity searching. Nucleic Acids Research, 2011,39:W29-W37. DOI: 10.1093/nar/gkr367. pmid: 21593126
[19]	CHEN C J, XIA R, CHEN H, HE Y H. TBtools, a toolkit for biologists integrating various HTS-data handling tools with a user-friendly interface. bioRxiv, 2018. DOI: https://doi.org/10.1101/ 289660. pmid: 32817944
[20]	艾嘉, 刘坤, 张蕾, 吴明旭, 陈硕, 朱德辉, 韩毅强, 高亚梅. 细菌漆酶的生物信息学分析. 基因组学与应用生物学, 2019,28(3):1070-1078.
	AI J, LIU K, ZHANG L, WU M X, CHEN S, ZHU D H, HAN Y Q, GAO Y M. Bioinformatics analysis of bacteria laccase. Genomics and Applied Biology, 2019,28(3):1070-1078. (in Chinese)
[21]	KUMAR S, STECHER G, TAMURA K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 2016,33(7):1870-1874. DOI: 10.1093/molbev/msw054. pmid: 27004904
[22]	张春晓, 王文棋, 蒋湘宁, 陈雪梅. 植物基因启动子研究进展. 遗传学报, 2004,31(12):1455-1464.
	ZHANG C X, WANG W Q, JIANG X N, CHEN X M. Review on plant gene promoters. Journal of Genetics and Genomics, 2004,31(12):1455-1464. (in Chinese)
[23]	李宗霆, 周燮. 植物激素及其免疫检测技术. 南京: 江苏科学技术出版社, 1996.
	LI Z T, ZHOU X. Plant Hormones and Their Immunological Detection Techniques. Nanjing: Jiangsu Science and Technology Press, 1996. (in Chinese)
[24]	REEN D J. Enzyme-linked immunosorbent assay (ELISA)//WALKER J M. Methods in Molecular Biology, 1994,32:461-466. doi: 10.1385/0-89603-268-X:461 pmid: 7951745
[25]	LANGFELDER P, HORVATH S. WGCNA: An R package for weighted correlation network analysis. BMC Bioinformatics, 2008,9:559. DOI: 10.1186/1471-2105-9-559. pmid: 19114008
[26]	SU G, MORRIS J H, DENCHAK B, BADER G D. Biological network exploration with Cytoscape 3. Current Protocols in Bioinformatics, 2014,47: 8.13.1-8.13.24. DOI: 10.1002/0471250953. bi0813s47.
[27]	DE VLEESSCHAUWER D, YANG Y N, CRUZ C V, HOFTE M. Abscisic acid-induced resistance against the brown spot pathogen Cochliobolus miyabeanus in rice involves MAP kinase-mediated repression of ethylene signaling. Plant Physiology, 2010,152:2036-2052. DOI: 10.1104/pp.109.152702. pmid: 20130100
[28]	ULFERTS S, DELVENTHAL R, SPLIVALLO R, KARLOVSKY P, SCHAFFRATH U. Abscisic acid negatively interferes with basal defence of barley againstMagnaporthe oryzae. BMC Plant Biology, 2015,15:e7. DOI: 10.1186/s12870-014-0409-x.
[29]	KOBAYASHI Y, YAMAMOTO S, MINAMI H, KAGAYA Y, HATTORI T. Differential activation of the rice sucrose nonfermenting 1-related protein kinase2 family by hyperosmotic stress and abscisic acid. The Plant Cell, 2004,16(5):1163-1177. DOI:10.1105/tpc. 019943. doi: 10.1105/tpc.019943 pmid: 15084714
[30]	BOUDSOCQ M, BARBIER-BRYGOO H, LAURIERE C. Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. Journal of Biological Chemistry, 2004,279:41758-41766. DOI: 10.1074/jbc.M405259200. pmid: 15292193
[31]	HUAI J, WANG M, HE J, ZHENG J, DONG Z, LV H, ZHAO J, WANG G. Cloning and characterization of the SnRK2 gene family from Zea mays. Plant Cell Reports, 2008,27:1861-1868. DOI:10.1007/s00299-008-0608-8. pmid: 18797872
[32]	LI L B, ZHANG Y R, LIU K C, NI Z F, FANG Z J, SUN Q X, GAO J W. Identification and bioinformatics analysis of SnRK2 and CIPK family genes in soghum. Agricultural Sciences in China, 2010,9(1):19-30. DOI: 10.1016/S1671-2927(09)60063-8.
[33]	田晓杰. 水稻ABA受体OsPYLs基因家族的鉴定和功能研究[D]. 北京: 中国科学院大学, 2017.
	TIAN X J. Characterization and functional analysis of pyrabactin resistance-like abscisic acid receptor family in rice. Beijing: University of Chinese Academy of Sciences, 2017. (in Chinese)
[34]	贾振华. 植物黄酮类化合物槲皮素与转录因子AtMYB44诱导和调控植物防卫反应的研究[D]. 南京: 南京农业大学, 2010.
	JIA Z H. Studies on roles of flavonoid quercetin and transcription factor AtMYB44 in induction and regulation of defense responses in Arabidopsis thaliana. Nanjing: Nanjing Agricultural University, 2010. (in Chinese)
[35]	CHANDA B, VENUGOPAL S C, KULSHRESTHA S, NAVARRE D A, DOWNIE B, VAILLANCOURT L, KACHROO A, KACHROO P. Glycerol-3-phosphate levels are associated with basal resistance to the hemibiotrophic fungus Colletotrichum higginsianum in Arabidopsis. Plant Physiology, 2008,147:2017-2029. DOI: 10.1104/pp.108. 121335. pmid: 18567828
[36]	CHASSOT C, NAWRATH C, METRAUX J P. Cuticular defects lead to full immunity to a major plant pathogen. The Plant Journal, 2007,49(6):972-980. DOI: 10.1111/j.1365-313X.2006.03017.x. pmid: 17257167
[37]	NAKASHITA H, YASUDA M, NITTA T, ASAMI T, FUJIOKA S, ARAI Y, SEKIMATA K, TAKATSUTO S, YAMAGUCHI I, YOSHIDA S. Brassinosteroid functions in a broad range of disease resistance in tobacco and rice. The Plant Journal, 2003,33(5):887-898. DOI: 10.1046/j.1365-313X.2003.01675.x. pmid: 12609030
[38]	李宝燕. 烟草WD40蛋白TTG2对生长发育和抗病性的调控作用[D]. 南京: 南京农业大学, 2012.
	LI B Y. Regulatory roles of WD40-domain protein TTG2 in growth, development and pathogen defense of tobacco[D]. Nanjing: Nanjing Agricultural University, 2012. (in Chinese)
[39]	JIANG Y J, YU D Q. The WRKY57 transcription factor affects the expression of jasmonate ZIM-domain genes transcriptionally to compromise Botrytis cinerea resistance. Plant Physiology, 2016,171(4):2771-2782. DOI: 10.1104/pp.16.00747. doi: 10.1104/pp.16.00747 pmid: 27268959
[40]	YU Y, WAN Y T, JIAO Z L, BIAN L, YU K K, ZHANG G H, GUO D L. Functional characterization of resistance to powdery mildew of VvTIFY9 from Vitis vinifera. International Journal of Molecular Sciences, 2019,20(17):4286. DOI: 10.3390/ijms20174286.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

类型 Type	基因编号 Gene ID	染色体 Chromosome	基因组位置 Genomic location	氨基酸长度 Amino acid (aa)	等电点 pI	分子量 Molecular weight (kD)	总平均疏水指数 Average of hydropathicity
PYL	Seita.1G013900	1	1185261-1188981	211	5.92	23.67	-0.393
	Seita.1G030500	1	2860241-2862675	201	5.97	21.72	-0.338
	Seita.3G072600	3	4600680-4602575	193	4.39	20.38	-0.011
	Seita.3G076200	3	4851939-4854302	205	5.91	22.16	-0.233
	Seita.3G207900	3	16201030-16202684	204	8.88	21.58	-0.132
	Seita.4G239500	4	36338582-36339202	206	6.71	22.14	-0.220
	Seita.5G140800	5	12377439-12378609	206	5.25	21.84	-0.101
	Seita.5G302400	5	35644268-35645327	175	4.93	18.81	-0.210
	Seita.5G369100	5	40592575-40593198	207	6.75	22.70	-0.252
	Seita.9G311900	9	36003871-36005478	207	5.24	22.13	-0.195
	Seita.9G437300	9	48993016-48994206	220	6.58	22.92	0.036
SnRK2	Seita.1G190000	1	27251818-27256180	454	8.49	51.77	-0.605
	Seita.2G394500	2	45956776-45961725	339	5.30	38.47	-0.177
	Seita.3G003200	3	157270-159276	380	5.99	43.11	-0.547
	Seita.3G230400	3	19092668-19097929	360	5.68	41.77	-0.546
	Seita.3G369900	3	47387035-47389674	374	4.94	41.46	-0.224
	Seita.5G395400	5	42440116-42444731	362	6.06	42.33	-0.619
	Seita.7G100500	7	20312278-20317276	358	6.00	40.98	-0.558
	Seita.9G318200	9	36623246-36626468	333	5.48	37.93	-0.467
	Seita.9G079800	9	4716246-4721220	366	4.81	41.48	-0.308
	Seita.9G169200	9	11473484-11476494	362	4.73	40.73	-0.283
	Seita.9G379000	9	43841287-43845704	344	5.10	39.13	-0.238

候选基因 Candidate gene	水稻同源基因 Homologous genes in O. sativa	基因功能 Gene function	拟南芥同源基因 Homologous genes in A. thaliana	基因功能 Gene function
Seita.4G105600	LOC_Os06g33480	包含蛋白质的WD结构域、G-beta重复结构域 WD domain, G-beta repeat domain containing protein	AT1G24130	转导蛋白/WD40重复超家族蛋白 Transduction/WD40 repeat-like superfamily protein
Seita.6G218100	LOC_Os12g01180	WRKY57转录因子 WRKY57 transcription factors	AT5G46780	包含VQ基序的蛋白 VQ motif-containing protein
Seita.9G138400	LOC_Os06g36670	TIFY 转录因子 TIYF transcription factors	AT1G51600	TIFY2A转录因子 TIYF2A transcription factors

Identification of Co-Expression Genes Related to Endogenous Abscisic Acid in Response to the Stress of Sclerospora graminicola by WGCNA in Foxtail Millet

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 11

References 40

Related Articles 8

Metrics

Comments

Recommended 0

[1]	YuXia WEN,Jian ZHANG,Qin WANG,Jing WANG,YueHong PEI,ShaoRui TIAN,GuangJin FAN,XiaoZhou MA,XianChao SUN. Cloning, Expression and Anti-TMV Function Analysis of Nicotiana benthamiana NbMBF1c [J]. Scientia Agricultura Sinica, 2022, 55(18): 3543-3555.
[2]	SONG SongQuan,LIU Jun,XU HengHeng,LIU Xu,HUANG Hui. ABA Metabolism and Signaling and Their Molecular Mechanism Regulating Seed Dormancy and Germination [J]. Scientia Agricultura Sinica, 2020, 53(5): 857-873.
[3]	ZHANG Xue,YANG HongKun,ZHENG Ting,XIAO Yun,MO Piao,FAN GaoQiong. Effects of Exogenous ABA on Pre-Harvest Sprouting Resistance and Quality of White and Red Wheat Cultivars [J]. Scientia Agricultura Sinica, 2020, 53(23): 4750-4763.
[4]	LI Ying,ZHAO JiHao,LI JinRong,QIAN BiChang,LIU ZhaoXin,GAO Fang,YANG DongQing,LI XiangDong. Effects of Exogenous 6-BA on Root Growth and Pod Yield of Flooded Peanut at Different Growth Stages [J]. Scientia Agricultura Sinica, 2020, 53(18): 3665-3678.
[5]	LI Xue, WU JueTian, WANG Yi, JIANG Hong, BI Yang, SI Min, ZHANG JingRong, XU Jie. Postharvest ABA Treatment Promote Wound Healing of Potato ‘Longshu No.3’ Tubers [J]. Scientia Agricultura Sinica, 2017, 50(20): 4003-4011.
[6]	ZHANG Wei-1, GAO Wei-2, CAO Zhen-1, HE Li-Shan-1, TAN Gui-Yu-1, WANG Bao-Min-1. Immunolocalization and Quantitation of ABA and IAA in the Organs of Wheat (Triticumaestivum L.) Under Drought Stress [J]. Scientia Agricultura Sinica, 2014, 47(15): 2940-2948.
[7]	ZHANG Guo-Bin, YU Ji-Hua, FENG Zhi, MA Yan-Xia, 吕Jian . Effects of Exogenous Nitric Oxide and Abscisic Acid on Photosynthesis Characteristics in Pepper Seedlings Under Autotoxicity Stress [J]. Scientia Agricultura Sinica, 2013, 46(10): 2076-2084.
[8]	PAN Li-ping,LI Yan,TANG Li-song. Growth and Allocation of Photosynthetic Produces in Cotton Under Alternative Partial Root-Zone Irrigation [J]. Scientia Agricultura Sinica, 2009, 42(8): 2982-2986 .