四川玉米小斑病菌交配型组成与育性分析

doi:10.3864/j.issn.0578-1752.2021.12.006

Abstract

Abstract:

【Objective】The objective of this study is to clarify the distribution of mating types and fertility of Cochliobolus heterostrophus (anamorph: Bipolaris maydis), and to elucidate the possibility of the sexual reproduction of C. heterostrophus isolates in natural fields.【Method】A total of 544 C. heterostrophus isolates were collected from different geographical regions in Sichuan and Yunnan provinces during 2013-2018. The mating type of these isolates was detected by a multiple PCR method, and two standard tested isolates with high combining ability were screened by cross of MAT1-1 and MAT1-2 isolates. Then the 544 C. heterostrophus isolates were tested for their mating type and fertility with two standard test isolates. The fertility was determined by the development of pseudothecia and asci.【Result】Among the 544 isolates, 286 ones were identified as MAT1-1 and 258 as MAT1-2, accounting for 52.57% and 47.43%, respectively. Chi-square test gave a good fit of 1﹕1 ratio (χ²=1.441, P=0.230), indicating that these two mating types distributed in balance in natural fields. In addition, the investigation also showed that two mating types were present in all sampling locations and they had a basically same population structure in different years. After crossing tested isolates with standard isolates MSRS-2-3 (MAT1-1) and DY-12-1-2 (MAT1-2), the percentage of fertile and sterile isolates was 88.79% and 11.21%, respectively, and an obvious fertility differentiation was observed, which were characterized by 12.32% of high fertile, 27.39% of medium fertile, and 49.08% of low fertile isolates, respectively. Fertility structures of isolates varied in different geographical locations and in different collection years. The frequencies of fertile isolates ranged from 77.88% to 94.87% in 2013-2018. The percentage of fertile isolates in Southern Yunnan was highest up to 100%, followed by those in Northern Sichuan, Central Sichuan, Southern Sichuan and Eastern Sichuan regions, accounting for 93.25%, 89.87%, 83.33% and 79.31%, respectively. The percentage of fertile isolates in Western Sichuan region was lowest (69.23%).【Conclusion】MAT1-1 and MAT1-2 achieved an equilibrium in quantity among C. heterostrophus field populations, and the cross combinations between MAT1-1 and MAT1-2 isolates were generally fertile. Although sexual generation was rarely reported in C. heterostrophus population in natural condition, the current results indicated that cryptic sexual cycle might contribute to the genetic diversity of C. heterostrophus.

Key words: southern corn leaf blight, Cochliobolus heterostrophus, sexual reproduction, mating type, fertility, multiple PCR

SUN XiaoFang,LIU Min,PAN TingMin,GONG GuoShu. Mating Type and Fertility of Cochliobolus heterostrophus Causing Southern Corn Leaf Blight in Sichuan Province[J].Scientia Agricultura Sinica, 2021, 54(12): 2547-2558.

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Figures/Tables 9

Table 1

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Table 4

Fertility of C. heterostrophus isolates collected from 17 cities (prefectures) of Sichuan and Xishuangbanna of Yunnan provinces"

采样区 Sampling region		测试菌株数 Number of tested isolates	不同假囊壳形成能力的菌株数（出现频率） Number of isolates with different pseudothecium-forming levels (Frequency, %)				可育菌株数（出现频率）Number of fertile isolates (Frequency, %)	不同育性水平的菌株数（出现频率） Number of isolates with different fertility levels (Frequency, %)
采样区 Sampling region		测试菌株数 Number of tested isolates	高 High	中 Medium	低 Low	无 Absent	可育菌株数（出现频率）Number of fertile isolates (Frequency, %)	高 High	中 Medium	低 Low	不育 Sterile
四川中部 Central Sichuan	成都Chengdu	161	33 (20.50)	59 (36.65)	65 (40.37)	4 (2.48)	145 (90.06)	21 (13.04)	56 (34.78)	68 (42.24)	16 (9.94)
四川中部 Central Sichuan	眉山Meishan	66	11 (16.67)	20 (30.30)	29 (43.94)	6 (9.09)	59 (89.39)	6 (9.09)	19 (28.79)	34 (51.52)	7 (10.61)
总计Total		227	44 (19.38)	79 (34.80)	94 (41.41)	10 (4.41)	204 (89.87)	27 (11.89)	75 (33.04)	102 (44.93)	23 (10.13)
四川东部 Eastern Sichuan	内江Neijiang	11	0 (0)	4 (36.36)	7 (63.63)	0 (0)	11 (100.00)	0 (0)	4 (36.36)	7 (63.63)	0 (0)
	遂宁Suining	11	0 (0)	3 (27.27)	8 (72.73)	0 (0)	8 (72.73)	0 (0)	3 (27.27)	5 (45.45)	3 (27.27)
	南充Nanchong	16	4 (25.00)	3 (18.75)	8 (50.00)	1 (6.25)	13 (81.25)	3 (18.75)	3 (18.75)	7 (43.75)	3 (18.75)
	广安Guang’an	12	2 (16.67)	2 (16.67)	6 (50.00)	2 (16.67)	9 (75.00)	1 (8.33)	3 (25.00)	5 (41.67)	3 (25.00)
	巴中Bazhong	3	0 (0)	1 (33.30)	1 (33.30)	1 (33.30)	2 (66.67)	0 (0)	1 (33.33)	1 (33.33)	1 (33.33)
	达州Dazhou	5	0 (0)	1 (20.00)	2 (40.00)	2 (40.00)	3 (60.00)	0 (0)	1 (20.00)	2 (40.00)	2 (40.00)
总计Total		58	6 (10.34)	14 (24.14)	32 (55.17)	6 (10.34)	46 (79.31)	4 (6.90)	15 (25.86)	27 (46.55)	12 (20.69)
四川西部Western Sichuan	雅安Yaan	11	4 (36.36)	4 (36.36)	2 (18.18)	1 (9.09)	9 (81.82)	2 (18.18)	2 (18.18)	5 (45.45)	2 (18.18)
四川西部Western Sichuan	甘孜州Garze	2	0 (0)	0 (0)	0 (0)	2 (100.00)	0 (0)	0 (0)	0 (0)	0 (0)	2 (100.00)
总计Total		13	4 (30.77)	4 (30.77)	2 (15.38)	3 (23.08)	9 (69.23)	2 (15.38)	2 (15.38)	5 (38.46)	4 (30.77)
四川南部Southern Sichuan	自贡Zigong	5	0 (0)	5 (100.00)	0 (0)	0 (0)	5 (100.00)	0 (0)	0 (0)	5 (100.00)	0 (0)
	乐山Leshan	40	8 (20.00)	9 (22.5)	23 (57.50)	0 (0.00)	35 (87.50)	4 (10.00)	5 (12.50)	26 (65.00)	5 (12.50)
	宜宾Yibin	7	2 (28.57)	1 (14.29)	2 (28.57)	2 (28.57)	5 (71.43)	0 (0)	0 (0)	5 (71.43)	2 (28.57)
	泸州Luzhou	14	0 (0)	4 (28.57)	7 (50.00)	3 (21.43)	10 (71.43)	0 (0)	4 (28.57)	6 (42.86)	4 (28.57)
总计Total		66	10 (15.15)	19 (28.79)	32 (48.48)	5 (7.58)	55 (83.33)	4 (6.06)	9 (13.64)	42 (63.64)	11 (16.67)
四川北部 Northern Sichuan	德阳Deyang	84	15 (17.86)	35 (41.67)	32 (38.10)	2 (2.38)	80 (95.24)	9 (10.71)	26 (30.95)	45 (53.57)	4 (4.76)
	绵阳Mianyang	56	11 (19.64)	17 (30.36)	23 (41.07)	5 (8.93)	51 (91.07)	9 (16.07)	12 (21.43)	30 (53.57)	5 (8.93)
	广元Guangyuan	23	5 (21.74)	5 (21.74)	11 (47.83)	2 (8.70)	21 (91.30)	2 (8.70)	5 (21.74)	14 (60.87)	2 (8.70)
总计Total		163	31 (19.02)	57 (34.97)	66 (40.49)	9 (5.52)	152 (93.25)	20 (12.27)	43 (26.38)	89 (54.60)	11 (6.75)
云南南部 Southern Yunnan	西双版纳州Xishuangbanna	17	10 (58.82)	5 (29.41)	2 (11.76)	0 (0)	17 (100.00)	10 (58.82)	5 (29.41)	2 (11.76)	0 (0)

Table 4

Table 5

Table 6

Table 7

References 44

[1]	ULLSTRUP A J. The impacts of the southern corn leaf blight epidemics of 1970-1971. Annual Review of Phytopathology, 1972,10:37-50. doi: 10.1146/annurev.py.10.090172.000345
[2]	MANAMGODA D S, CAI L, BAHKALI A H, CHUKEATIROTE E, HYDE K D. Cochliobolus: An overview and current status of species. Fungal Diversity, 2011,51(1):3-42. doi: 10.1007/s13225-011-0139-4
[3]	BENGYELLA L, YEKWA E L, NAWAZ K, IFTIKHAR S, TAMBO E, ALISOLTANI A, FETO N A, ROY P. Global invasive Cochliobolus species: Cohort of destroyers with implications in food losses and insecurity in the twenty-first century. Archives of Microbiology, 2018,200(1):119-135. doi: 10.1007/s00203-017-1426-6
[4]	王利智, 康志钰, 吴毅歆, 周惠萍, 毛自朝, 何月秋. 云南省玉米小斑病菌生理小种的初步鉴定. 云南大学学报(自然科学版), 2010,32(3):352-357.
	WANG L Z, KANG Z Y, WU Y X, ZHOU H P, MAO Z C, HE Y Q. Preliminary identification of physiological races of Bipolaris maydis in Yunnan. Journal of Yunnan University (Natural Science Edition), 2010,32(3):352-357. (in Chinese)
[5]	赵聚莹, 蒋晓丽, 贾海民, 李术臣, 石洁, 张海剑. 黄淮海地区玉米小斑病菌生理小种鉴定与评价. 河北农业科学, 2012,16(9):47-49.
	ZHAO J Y, JIANG X L, JIA H M, LI S C, SHI J, ZHANG H J. Identification and evaluation of physiological races of Bipolaris maydis in Huanghuihai region. Journal of Hebei Agricultural Sciences, 2012,16(9):47-49. (in Chinese)
[6]	陆宁海, 吴利民, 郎剑锋, 霍云凤, 石明旺. 河南省玉米小斑病菌生理小种鉴定及致病力分化. 湖北农业科学, 2015,54(7):1603-1606.
	LU N H, WU L M, LANG J F, HUO Y F, SHI M W. Identification of physiological races and pathogenicity differentiation of Bipolaris maydis in Henan Province. Hubei Agricultural Sciences, 2015,54(7):1603-1606. (in Chinese)
[7]	孔令晓, 赵聚莹, 栗秋生, 王连生, 罗畔池. 河北省玉米小斑病菌生理小种鉴定及群体动态变化. 华北农学报, 2005,20(3):90-93.
	KONG L X, ZHAO J Y, LI Q S, WANG L S, LUO P C. Identification and population dynamics of physiological races of Bipolaris maydis in Hebei. Acta Agriculturae Boreali-Sinica, 2005,20(3):90-93. (in Chinese)
[8]	WANG M, WANG S Q, MA J, YU C J, GAO J X, CHEN J. Detection of Cochliobolus heterostrophus races in South China. Journal of Phytopathology, 2017,165(10):681-691. doi: 10.1111/jph.2017.165.issue-10
[9]	甘林, 代玉立, 阮宏椿, 石妞妞, 杜宜新, 陈福如, 杨秀娟. 福建省玉米小斑病菌的致病型及其群体结构分析. 中国农学通报, 2018,34(7):147-151.
	GAN L, DAI Y L, RUAN H C, SHI N N, DU Y X, CHEN F R, YANG X J. Pathotype and its population structure of Cochliobolus heterostrophus in Fujian Province. Chinese Agricultural Science Bulletin, 2018,34(7):147-151. (in Chinese)
[10]	DAI Y L, GAN L, RUAN H C, SHI N N, DU Y X, CHEN F R, YANG X J. Characterization of natural isolates of Bipolaris maydis associated with mating types, genetic diversity, and pathogenicity in Fujian Province, China. Plant Disease, 2020,104(2):323-329. doi: 10.1094/PDIS-03-19-0650-RE
[11]	NI M, FERETZAKI M, SUN S, WANG X Y, HEITMAN J. Sex in fungi. Annual Review of Genetics, 2011,45:405-430. doi: 10.1146/annurev-genet-110410-132536
[12]	WHITTLE C A, NYGREN K, JOHANNESSON H. Consequences of reproductive mode on genome evolution in fungi. Fungal Genetics and Biology, 2011,48(7):661-667. doi: 10.1016/j.fgb.2011.02.005
[13]	WILKEN P M, STEENKAMP E T, WINGFIELD M J, DE BEER Z W, WINGFIELD B D. Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered. Fungal Biology Reviews, 2017,31(4):199-211. doi: 10.1016/j.fbr.2017.05.003
[14]	NELSON R R. A major gene locus for compatibility in Cochliobolus heterostrophus. Phytopathology, 1957,47:742-743.
[15]	TURGEON B G, BOHLMANN H, CIUFFETTI L M, CHRISTIANSEN S K, YANG G, SCHAFER W, YODER O C. Cloning and analysis of the mating type genes from Cochliobolus heterostrophus. Molecular and General Genetics, 1993,238:270-284.
[16]	MILLER J S, JOHNSON D A, HAMM P B. Aggressiveness of isolates of Phytophthora infestans from the Columbia Basin of Washington and Oregon. Phytopathology, 1998,88(3):190-197. doi: 10.1094/PHYTO.1998.88.3.190
[17]	VARGAS A M, OCAMPO L M, CÉSPEDES M C, CARREÑO N, GONZÁLEZ A, ROJAS A, ZULUAGA A P, MYERS K, FRY W E, JIMÉNEZ P, BERNAL A J, RESTREPO S. Characterization of Phytophthora infestans populations in Colombia: First report of the A2 mating type. Phytopathology, 2009,99(1):82-88. doi: 10.1094/PHYTO-99-1-0082
[18]	张晓玉, 张亚玲, 靳学慧, 周弋力, 孟峰, 武菁菁. 黑龙江省稻瘟病菌育性及其交配型分析. 植物保护学报, 2020,47(1):93-100.
	ZHANG X Y, ZHANG Y L, JIN X H, ZHOU Y L, MENG F, WU J J. Analysis of the fertility and mating type of rice blast fungus Magnaporthe oryzae in Heilongjiang Province. Journal of Plant Protection, 2020,47(1):93-100. (in Chinese)
[19]	SALEH D, XU P, SHEN Y, LI C Y, ADREIT H, MILAZZO J, RAVIGNÉ V, BAZIN E, NOTTÉGHEM J, FOURNIER E, THARREAU D. Sex at the origin: An Asian population of the rice blast fungus Magnaporthe oryzae reproduces sexually. Molecular Ecology, 2012,21(6):1330-1344. doi: 10.1111/j.1365-294X.2012.05469.x
[20]	SALEH D, MILAZZO J, ADREIT H, FOURNIER E, THARREAU D. South-East Asia is the center of origin, diversity and dispersion of the rice blast fungus, Magnaporthe oryzae. New Phytologist, 2014,201(4):1440-1456. doi: 10.1111/nph.2014.201.issue-4
[21]	ZHONG Z H, CHEN M L, LIN L Y, HAN Y J, BAO J D, TANG W, LIN L L, LIN Y H, SOMAI R, LU L, et al. Population genomic analysis of the rice blast fungus reveals specific events associated with expansion of three main clades. The ISME Journal, 2018,12(8):1867-1878. doi: 10.1038/s41396-018-0100-6
[22]	戴冬青, 王绍新, 刘宁, 贾慧, 曹志艳, 董金皋. 玉米大斑病菌交配型组成鉴定及其有性生殖条件优化. 植物保护学报, 2019,46(3):634-641.
	DAI D Q, WANG S X, LIU N, JIA H, CAO Z Y, DONG J G. Identification of mating type and optimization of sexual reproduction conditions of northern leaf blight fungus Setosphaeria turcica. Journal of Plant Protection, 2019,46(3):634-641. (in Chinese)
[23]	代玉立, 甘林, 滕振勇, 杨静民, 祁月月, 石妞妞, 陈福如, 杨秀娟. 玉米大斑病菌和小斑病菌交配型多重PCR检测方法的建立与应用. 中国农业科学, 2020,53(3):527-538.
	DAI Y L, GAN L, TENG Z Y, YANG J M, QI Y Y, SHI N N, CHEN F R, YANG X J. Establishment and application of a multiple PCR method to detect mating types of Exserohilum turcicum and Bipolaris maydis. Scientia Agricultura Sinica, 2020,53(3):527-538. (in Chinese)
[24]	LU Y Y, LIU K X, LI G F, YAN L B, XIAO S Q, XUE C S. Identification mating-type locus structure and distribution of Cochliobolus lunatus in China. European Journal of Plant Pathology, 2018,151(2):487-500. doi: 10.1007/s10658-017-1393-4
[25]	乔广行, 李兴红, 黄金宝, 林秀敏, 周莹. 灰葡萄孢交配型基因的分析与检测. 菌物学报, 2015,34(1):108-116.
	QIAO G Y, LI X H, HUANG J B, LIN X M, ZHOU Y. Analysis and molecular detection of Botrytis cinerea mating type genes. Mycosystema, 2015,34(1):108-116. (in Chinese)
[26]	PEI Y G, TAO Q J, ZHENG X J, LI Y, SUN X F, LI Z F, QI X B, XU J, ZHANG M, CHEN H B, CHANG X L, TANG H M, SUI L Y, GONG G S. Phenotypic and genetic characterization of Botrytis cinerea population from kiwifruit in Sichuan Province, China. Plant Disease, 2019,103(4):748-758. doi: 10.1094/PDIS-04-18-0707-RE
[27]	史文琦, 龚双军, 曾凡松, 薛敏峰, 杨立军, 喻大昭. 小麦白粉病菌有性生殖与自然群体交配型检测. 植物病理学报, 2016,46(5):645-652.
	SHI W Q, GONG S J, ZENG F S, XUE M F, YANG L J, YU D Z. Sexual reproduction and detection of mating-type of Blumeria graminis f. sp. tritici populations. Acta Phytopathologica Sinica, 2016,46(5):645-652. (in Chinese)
[28]	SUN X F, QI X B, WANG W, LIU X, ZHAO H N, WU C P, CHANG X L, ZHANG M, CHEN H B, GONG G S. Etiology and symptom of corn leaf spot caused by Bipolaris spp. in Sichuan Province. Pathogens, 2020,9(3):229. doi: 10.3390/pathogens9030229
[29]	李文强, 王源超, 郑小波. 宁夏稻瘟病菌的交配型与育性. 中国水稻科学, 2007,21(6):650-656.
	LI W Q, WANG Y C, ZHENG X B. Mating type and fertility of Magnaporthe grisea population from rice in Ningxia Hui Autonomous Region, China. Chinese Journal of Rice Science, 2007,21(6):650-656. (in Chinese)
[30]	HUMAN M P, BARNES I, CRAVEN M, CRAMPTON B G. Lack of population structure and mixed reproduction modes in Exserohilum turcicum from South Africa. Phytopathology, 2016,106(11):1386-1392. doi: 10.1094/PHYTO-12-15-0311-R
[31]	杨阳, 马双新, 贾慧, 刘微微, 曹志艳, 董金皋. 玉米大斑病菌两性交配型菌株的出现频率及其育性分析. 植物保护学报, 2015,42(6):921-926.
	YANG Y, MA S X, JIA H, LIU W W, CAO Z Y, DONG J G. The occurrence and fertility analysis of bisexual strains of Setosphaeria turcica. Journal of Plant Protection, 2015,42(6):921-926. (in Chinese)
[32]	MEYLING N V, LUBECK M, BUCKLEY E P, EILENBERG J, REHNER S A. Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Molecular Ecology, 2009,18(6):1282-1293. doi: 10.1111/mec.2009.18.issue-6
[33]	GUO L Y, KO W H. Continuing variation in successive asexual generations of Pythium splendens following sexual reproduction. Mycological Research, 1995,99(11):1339-1344. doi: 10.1016/S0953-7562(09)81218-5
[34]	胡旭章, 黄梧芳. 玉米小斑病菌(Drechslera maydis)与玉米圆斑病菌(Drechslera carbonum)种间杂交的初步研究. 河北农业大学学报, 1992,15(2):8-12.
	HU X Z, HUANG W F. Preliminary study on interspecific crossing of Drechslera (=Helminthosoporium) maydis and Drechslera (=Helminthosoporium) carbonum. Journal of Hubei Agricultural University, 1992,15(2):8-12. (in Chinese)
[35]	王宝华, 李海明, 鲁国东, 王宗华. 稻瘟病菌群体育性分析的主要影响因子研究. 植物病理学报, 2007,37(2):144-150.
	WANG B H, LI H M, LU G D, WANG Z H. Factors affecting population fertility analysis of Magnaporthe grises. Acta Phytopathologica Sinica, 2007,37(2):144-150. (in Chinese)
[36]	NELSON R R. Genetics of Cochliobolus heterostrophus. II. Genetic factors inhibiting ascospore formation. Mycologia, 1959,51(1):24-30. doi: 10.1080/00275514.1959.12024792
[37]	BRONSON C R. Ascospore abortion in crosses of Cochliobolus heterostrophus heterozygous for the virulence locus Tox1. Genome, 1988,30(1):12-18. doi: 10.1139/g88-003
[38]	RAJU N B. Meiosis and ascospore development in Cochliobolus heterostrophus. Fungal Genetics and Biology, 2008,45(4):554-564. doi: 10.1016/j.fgb.2007.08.007
[39]	WIRSEL S, HORWITZ B, YAMAGUCHI K, YODER O C, TURGEON B G. Single mating type-specific genes and their 3′ UTRs control mating and fertility in Cochliobolus heterostrophus. Molecular and General Genetics, 1998,259(3):272-281. doi: 10.1007/s004380050813
[40]	HORWITZ B A, SHARON A, LU S W, RITTER V, SANDROCK T M, YODER O C, TURGEON B G. A G protein alpha subunit from Cochliobolus heterostrophus involved in mating and appressorium formation. Fungal Genetics and Biology, 1999,26(1):19-32. doi: 10.1006/fgbi.1998.1094
[41]	GANEM S, LU S W, LEE B N, CHOU D Y T, HADAR R, TURGEON B G, HORWITZ B A. G-protein β subunit of Cochliobolus heterostrophus involved in virulence, asexual and sexual reproductive ability, and morphogenesis. Eukaryotic Cell, 2004,3(6):1653-1663. doi: 10.1128/EC.3.6.1653-1663.2004
[42]	WU D, OIDE S, ZHANG N, CHOI M Y, TURGEON B G. ChLae1 and ChVel1 regulate T-toxin production, virulence, oxidative stress response, and development of the maize pathogen Cochliobolus heterostrophus. PLoS Pathogens, 2012,8(2):e1002542. doi: 10.1371/journal.ppat.1002542
[43]	WANG W W, WU D L, PAN H Y, TURGEON B G. Vel2 and Vos1 hold essential roles in ascospore and asexual spore development of the heterothallic maize pathogen Cochliobolus heterostrophus. Fungal Genetics and Biology, 2014,70:113-124. doi: 10.1016/j.fgb.2014.07.010
[44]	SUMITA T, IZUMITSU K, TANAKA C. Characterization of the autophagy-related gene BmATG8 in Bipolaris maydis. Fungal Biology, 2017,121(9):785-797. doi: 10.1016/j.funbio.2017.05.008

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假囊壳数量 Number of pseudothecia (dish)	假囊壳的形成能力等级 Pseudothecium-forming level	每个假囊壳中子囊的数量 Number of asci per pseudothecium	育性等级 Fertility level
＞200	高High	0	不育Sterile
		1-100	低Low
		101-200	中等Medium
		＞200	高High
100-200	中等 Medium	0	不育Sterile
		1-200	低Low
		＞200	中等Medium
1-100	低Low	0	不育Sterile
1-100	低Low	≥1	低Low
0	不育Sterile	—	不育Sterile

测试菌株 Tested isolate		MAT1-2
测试菌株 Tested isolate		9-10-7-2	A10	B73-2-2	C33	DY-12-1-2	CZ-1-2	S33-1-1	YN-1-1	YN-3-3	ZJ-10-2
MAT1-1	9-5-1	++	+		+	++			++	+	++
	C24	+	++^※	++	+^#		+	+	+	+	++
	MSRS-2-3	++	++		++
	CZ-10-2	++	++	++	+				++	++	++
	D4-4-1	++	++	++	+		++	++			++
	M17-1-2	++	++	++	++		++				++
	T91-1-2	++	++	++	++		++			++	++
	YN-3-2	++	++	++	++		++	++	++	++
	YN-6-1	++	++	++	++		++	++	++	++	++
	ZJ-7-1	++	++	++	++			++	++	++	++

采样年份 Sampling year	采样点数 Number of collection sites	测试菌株数 Number of tested isolates	MAT1-1菌株数Number of isolates of MAT1-1	MAT1-2菌株数Number of isolates of MAT1-2	MAT1-1﹕MAT1-2	可育菌株数Number of fertile isolates	可育菌株率 Percentage of fertile isolates (%)	不同育性水平的菌株数（出现频率） Number of isolates with different fertility levels (Frequency, %)
采样年份 Sampling year	采样点数 Number of collection sites	测试菌株数 Number of tested isolates	MAT1-1菌株数Number of isolates of MAT1-1	MAT1-2菌株数Number of isolates of MAT1-2	MAT1-1﹕MAT1-2	可育菌株数Number of fertile isolates	可育菌株率 Percentage of fertile isolates (%)	高 High	中 Medium	低 Low	不育 Sterile
2013	29	113	63	50	1.13﹕1	88	77.88	8 (7.08)	23 (20.35)	57 (50.44)	25 (22.12)
2014	9	28	14	14	1﹕1	22	78.57	4 (14.29)	8 (28.57)	10 (35.71)	6 (21.43)
2015	16	60	28	32	1﹕1.14	56	93.33	4 (6.67)	26 (43.33)	26 (43.33)	4 (6.67)
2016	18	39	17	22	1﹕1.29	37	94.87	4 (10.26)	11 (41.03)	22 (43.59)	2 (5.13)
2017	23	215	123	92	1.34﹕1	201	93.49	26 (12.09)	59 (27.44)	116 (53.95)	14 (6.51)
2018	8	89	41	48	1﹕1.17:	79	88.76	21 (23.60)	22 (24.72)	36 (40.45)	10 (11.24)
总计Total	103	544	286	258	1.11﹕1	483	88.79	67 (12.32)	149 (27.39)	267 (49.08)	61 (11.21)

测试菌株 Tested isolate		MAT1-1
测试菌株 Tested isolate		CZ-4-1	CZ-1-1	18LS-3-3	D4-1-3	18CZ-3-2	JY-2-1-1	YN-3-2	N1-1	18LS-3-10	GY-4-2-1
MAT1-2	B73-2-2		^※
	J-A	++	++	+^※	+	++	+			++	+
	YN-5-3
	DJY-1-2A	++	++	++	+		++				++
	MY-13-2-2			++	++
	18CZ-2-2
	ZJ-10-2	+		++	+	+	++	++	++		+
	MY-11-2-3				++
	CZ-5-2				++	++					++
	RS-1A				++		++		++

测试菌株 Tested isolate		MAT1-1
测试菌株 Tested isolate		XD-1-2-1	J1-3	WJ-1-3	GY-6-1-2	GY-1-1-1	D4-3-1	X3-1-1	MS-6-1-1	CZ-7-1-1	QL-1-2A	MS-7-1-1
MAT1-2	RS-2-2	+	+	++	+^#	+	+^#	++	+^#	++^#	+	++
	18LS-2-5	++	++	++	+^#	++	–	++	+		++	++
	YLS-3-3	+	+	++	+^#	+	–	+^#	+^#	+	+
	18LS-2-3	++	++	+	+^#	++^#	+^#	+	+^#	++^#	++^#	++^#
	GH-5-1-1	++^※	+	+	+^#	+^#	–	+^#	+^#	++^#	+	+
	P4-1-1	+	+	+	+^#	+^#	–	–	+^#	+^#	++^#	++
	ZJ-8-1	++	++	++	+^#	++	++^#	++	++^#	++		++
	JY-1-2-1	+	++	++	+^#	++	–	+	+^#	++	++	++

Mating Type and Fertility of Cochliobolus heterostrophus Causing Southern Corn Leaf Blight in Sichuan Province

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采样区 Sampling region		采样点数 Number of collection sites	测试菌株数Number of tested isolates	MAT1-1		MAT1-2		MAT1-1﹕MAT1-2
采样区 Sampling region		采样点数 Number of collection sites	测试菌株数Number of tested isolates	测试菌株数 Number of tested isolates	比例 Percentage (%)	测试菌株数Number of tested isolates	比例 Percentage (%)	MAT1-1﹕MAT1-2
四川中部 Central Sichuan	成都Chengdu	15	161	85	52.80	76	47.20	1.12﹕1
四川中部 Central Sichuan	眉山Meishan	11	66	39	59.09	27	40.91	1.44﹕1
总计Total		26	227	124	54.63	103	45.37	1.2﹕1
四川东部 Eastern Sichuan	内江Neijiang	4	11	7	63.64	4	36.36	1.75﹕1
	遂宁Suining	4	11	3	27.27	8	72.73	1﹕2.67
	南充Nanchong	6	16	11	68.75	5	31.25	2.2﹕1
	广安Guang’an	5	12	5	41.67	7	58.33	1﹕1.4
	巴中Bazhong	2	3	2	66.70	1	33.30	2﹕1
	达州Dazhou	3	5	2	40.00	3	60.00	1﹕1.5
总计Total		24	58	30	51.72	28	48.28	1.07﹕1
四川西部 Western Sichuan	雅安Yaan	9	11	6	54.55	5	45.45	1.2﹕1
四川西部 Western Sichuan	甘孜州Garze	1	2	2	100.00	0	0	/
总计Total		10	13	8	61.54	5	38.46	1.6﹕1
四川南部 Southern Sichuan	自贡Zigong	2	5	3	60.00	2	40.00	1.5﹕1
	乐山Leshan	8	40	23	57.50	17	42.50	1.35﹕1
	宜宾Yibin	3	7	5	71.43	2	28.57	2.5﹕1
	泸州Luzhou	3	14	6	42.86	8	57.14	1﹕1.33
总计Total		16	66	37	56.06	29	43.94	1.28﹕1
四川北部 Northern Sichuan	德阳Deyang	8	84	36	42.86	48	57.14	1﹕1.33
	绵阳Mianyang	11	56	29	51.79	27	48.21	1.07﹕1
	广元Guangyuan	7	23	15	65.22	8	34.78	1.88﹕1
总计Total		26	163	80	49.08	83	50.92	1﹕1.04
云南南部 Southern Yunnan	西双版纳州Xishuangbanna	1	17	7	41.18	10	58.82	1﹕1.43

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