Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 4058-4065.doi: 10.3864/j.issn.0578-1752.2013.19.011

• PLANT PROTECTION • Previous Articles     Next Articles

Analysis on Mating Type and Parasitic Fitness Diversity in Sexual Hybridization Offsprings of Setosphaeria turcica

 GUO  Li-Yuan-1, JIA  Hui-1, CAO  Zhi-Yan-1, GU  Shou-Qin-1, SUN  Shu-Qin-2, DONG  Jin-Gao-1   

  1. 1.Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, Hebei
    2.Tianjin Institute of Plant Protection, Tianjin 300381
  • Received:2013-03-28 Online:2013-10-01 Published:2013-05-02

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Abstract: 【Objective】The objective of this study is to analyze the diversity of mating type and pathogenicity in sexual hybridization offsprings of Setosphaeria turcica and investigate the relationship between sexual hybridization and variation of S. turcica.【Method】Using isolates 01-12 and 01-15 of S. turcica as parents, the sexual hybridization F1 generations were obtained by artificial induction, and F2 generation isolates were got by the same method when isolates 40 and 42 coming from F1 generation were used as parents. The mating type and parasitic fitness of sexual hybridization offsprings were tested with Huangzaosi. The toxin of different pathogenic isolates was analyzed by capillary electrophoresis. 【Result】Two sexual generations were induced continuously. Seventy-nine sexual hybridization F1 and thirty-two F2 isolates were obtained in this experiment. The mating types were A, a, Aa and neutral isolates, which appeared obvious diversity, but the ratio of A and a in F1 generations was far away from 1:1. The result of parasitic fitness showed that F1 and F2 isolates generated diversity. The percentages of strong parasitic fitness and feeble parasitic fitness in F1 generations were 30.00% and 50.00%, respectively, and those in F2 generations were 21.87% and 31.25%, respectively. The results of capillary electrophoresis suggested that the toxin content of stronger pathogenic isolates were higher than that of lower pathogenic isolates. 【Conclusion】 The sexual hybridization is one of the chief elements resulting in mat type and pathogenic diversity. The toxin content of an isolate is positively correlated with its pathogenicity.

Key words: Setosphaeria turcica , sexual hybridization , mating type , parasitic fitness

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