马铃薯晚疫病菌对氟吡菌胺抗性突变体的获得及其生物学性状

doi:10.3864/j.issn.0578-1752.2016.19.006

Abstract

Abstract: 【Objective】The objectives of this study are to research the cross resistance between fluopicolide and the other fungicides and assess the resistance risk of Phytophthora infestans to fluopicolide. 【Method】The fluopicolide-resistant mutants were acquired by UV irradiating sporangia, UV irradiating mycelia and fungicide taming. The mutation frequency was calculated, the resistance levels were measured, whether the resistance could be inherited stably under the condition of no fungicide after 10 generations was studied, the fitness (including mycelial growth rates, sporulation capacity, and comprehensive fitness index) on RSA medium and detached leaves were measured and the competitiveness of those resistant mutants and their parental isolates were studied. Cross resistance between fluopicolide and other fungicides were analyzed. The resistance risk of P. infestans to fluopicolide was assessed by the resistance risk quantitative evaluation criteria which was created by the authors’ lab. 【Result】Twenty-one fluopicolide-resistant mutants were acquired in this study. The resistance levels of those resistant mutants ranged from 61 to 3 157 and the mutation frequency of UV irradiating sporangia was 2.78×10^-7. The fitness of most of the tested resistant mutants showed no significant difference with their respective parental isolates. In the test of competitiveness, among the 18 resistance frequency measurements of 1st, 3rd and 7th generations of two fluopicolide-resistant mutants, 3 were significantly lower than their parental isolates, 4 were significantly higher than their parental isolates, while the other 11 showed no difference. The resistance of all those 21 fluopicolide-resistance mutants could be inherited stably. The resistance of all those 21 fluopicolide-resistance mutants could be inherited stably. The correlation coefficient (r) between the lgEC₅₀ of the fluopicolide-resistant mutants and the fluopicolide-sensitive isolates to fluopicolide and the lgEC₅₀ of those isolates to azoxystrobin, pyraclostrobin, cymoxanil, dimethomorph, mandipropamid, metalaxyl and fluoride ether bacteria amide were 0.104 (p =0.654), 0.311 (p =0.170), 0.228 (p =0.081), 0.376 (p =0.093), 0.214 (p =0.351), 0.122 (p =0.599), 0.963 (p =0.000). The inherent resistance risk value of P. infestans to fluopicolide was 15.【Conclusion】The cross-resistance relationship was found between fluopicolide and fluoride ether bacteria amide. No cross-resistance relationship was found between fluopicolide and azoxystrobin, pyraclostrobin, cymoxanil, dimethomorph, mandipropamid and metalaxyl. There could be high inherent risk of P. infestans developing resistance to fluopicolide. The risk management of P. infestans to fuopicolide should be enhanced. It is suggested that the fluopicolide should be used alternately or in mixture with other not cross-resistant fungicides.

Key words: Phytophthora infestans, fluopicolide, resistant mutants, biological characteristics, cross resistance, inherent risk

LUO Yan-tao, MENG Run-jie, ZHAO Jian-jiang, HAN Xiu-ying, MA Zhi-qiang, WANG Wen-qiao, ZHANG Xiao-feng. Acquisition and Biological Characteristics of Fluopicolide-Resistant Isolates in Phytophthora infestans[J].Scientia Agricultura Sinica, 2016, 49(19): 3733-3745.

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Acquisition and Biological Characteristics of Fluopicolide-Resistant Isolates in Phytophthora infestans

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